Time-lapse imaging of Drosophila testis for monitoring actin dynamics and sperm release

Here we describe a simple step-by-step protocol for collecting high-resolution, time-lapse images of intact Drosophila testis ex vivo for a limited period using a confocal microscope, with minimum photo-toxic damage, to monitor spermatid individualization, coiling, and release. The F-actin dynamics...

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Detalles Bibliográficos
Autores principales: Kapoor, Tushna, Dubey, Pankaj, Ray, Krishanu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8689351/
https://www.ncbi.nlm.nih.gov/pubmed/34977674
http://dx.doi.org/10.1016/j.xpro.2021.101020
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author Kapoor, Tushna
Dubey, Pankaj
Ray, Krishanu
author_facet Kapoor, Tushna
Dubey, Pankaj
Ray, Krishanu
author_sort Kapoor, Tushna
collection PubMed
description Here we describe a simple step-by-step protocol for collecting high-resolution, time-lapse images of intact Drosophila testis ex vivo for a limited period using a confocal microscope, with minimum photo-toxic damage, to monitor spermatid individualization, coiling, and release. The F-actin dynamics during spermatid morphogenesis can be further investigated through laser ablations, Fluorescence-Recovery-After-Photobleaching, and drug treatments, using this protocol. For complete details on the use and execution of this protocol, please refer to Dubey et al. (2016), Dubey et al. (2019), and Kapoor et al. (2021).
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spelling pubmed-86893512021-12-30 Time-lapse imaging of Drosophila testis for monitoring actin dynamics and sperm release Kapoor, Tushna Dubey, Pankaj Ray, Krishanu STAR Protoc Protocol Here we describe a simple step-by-step protocol for collecting high-resolution, time-lapse images of intact Drosophila testis ex vivo for a limited period using a confocal microscope, with minimum photo-toxic damage, to monitor spermatid individualization, coiling, and release. The F-actin dynamics during spermatid morphogenesis can be further investigated through laser ablations, Fluorescence-Recovery-After-Photobleaching, and drug treatments, using this protocol. For complete details on the use and execution of this protocol, please refer to Dubey et al. (2016), Dubey et al. (2019), and Kapoor et al. (2021). Elsevier 2021-12-15 /pmc/articles/PMC8689351/ /pubmed/34977674 http://dx.doi.org/10.1016/j.xpro.2021.101020 Text en © 2021 Tata Institute of Fundamental Research, Mumbai, India https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Kapoor, Tushna
Dubey, Pankaj
Ray, Krishanu
Time-lapse imaging of Drosophila testis for monitoring actin dynamics and sperm release
title Time-lapse imaging of Drosophila testis for monitoring actin dynamics and sperm release
title_full Time-lapse imaging of Drosophila testis for monitoring actin dynamics and sperm release
title_fullStr Time-lapse imaging of Drosophila testis for monitoring actin dynamics and sperm release
title_full_unstemmed Time-lapse imaging of Drosophila testis for monitoring actin dynamics and sperm release
title_short Time-lapse imaging of Drosophila testis for monitoring actin dynamics and sperm release
title_sort time-lapse imaging of drosophila testis for monitoring actin dynamics and sperm release
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8689351/
https://www.ncbi.nlm.nih.gov/pubmed/34977674
http://dx.doi.org/10.1016/j.xpro.2021.101020
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AT dubeypankaj timelapseimagingofdrosophilatestisformonitoringactindynamicsandspermrelease
AT raykrishanu timelapseimagingofdrosophilatestisformonitoringactindynamicsandspermrelease

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