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Time-lapse imaging of Drosophila testis for monitoring actin dynamics and sperm release
Here we describe a simple step-by-step protocol for collecting high-resolution, time-lapse images of intact Drosophila testis ex vivo for a limited period using a confocal microscope, with minimum photo-toxic damage, to monitor spermatid individualization, coiling, and release. The F-actin dynamics...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8689351/ https://www.ncbi.nlm.nih.gov/pubmed/34977674 http://dx.doi.org/10.1016/j.xpro.2021.101020 |