Purification and preparation of Rhodobacter sphaeroides reaction centers for photocurrent measurements and atomic force microscopy characterization

The formation of defined surfaces consisting of photosynthetic reaction centers (RCs) in biohybrid solar cells is challenging. Here, we start with the production of engineered RCs for oriented binding. RCs are deposited onto gold electrodes, and 6-mercapto-1-hexanol (MCH) is used to displace multila...

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Autores principales: Jun, Daniel, Zhang, Sylvester, Grzędowski, Adrian Jan, Mahey, Amita, Beatty, J. Thomas, Bizzotto, Dan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8689352/
https://www.ncbi.nlm.nih.gov/pubmed/34977685
http://dx.doi.org/10.1016/j.xpro.2021.101044
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author Jun, Daniel
Zhang, Sylvester
Grzędowski, Adrian Jan
Mahey, Amita
Beatty, J. Thomas
Bizzotto, Dan
author_facet Jun, Daniel
Zhang, Sylvester
Grzędowski, Adrian Jan
Mahey, Amita
Beatty, J. Thomas
Bizzotto, Dan
author_sort Jun, Daniel
collection PubMed
description The formation of defined surfaces consisting of photosynthetic reaction centers (RCs) in biohybrid solar cells is challenging. Here, we start with the production of engineered RCs for oriented binding. RCs are deposited onto gold electrodes, and 6-mercapto-1-hexanol (MCH) is used to displace multilayers and non-specifically adsorbed RCs. The resulting electrode surfaces are analyzed for photocurrent generation using an intensity-modulated light and lock-in amplifier. Atomic force microscopy (AFM) is used to characterize the surface and the formation of RC structural assemblies. For complete details on the use and execution of this profile, please refer to Jun et al. (2021).
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spelling pubmed-86893522021-12-30 Purification and preparation of Rhodobacter sphaeroides reaction centers for photocurrent measurements and atomic force microscopy characterization Jun, Daniel Zhang, Sylvester Grzędowski, Adrian Jan Mahey, Amita Beatty, J. Thomas Bizzotto, Dan STAR Protoc Protocol The formation of defined surfaces consisting of photosynthetic reaction centers (RCs) in biohybrid solar cells is challenging. Here, we start with the production of engineered RCs for oriented binding. RCs are deposited onto gold electrodes, and 6-mercapto-1-hexanol (MCH) is used to displace multilayers and non-specifically adsorbed RCs. The resulting electrode surfaces are analyzed for photocurrent generation using an intensity-modulated light and lock-in amplifier. Atomic force microscopy (AFM) is used to characterize the surface and the formation of RC structural assemblies. For complete details on the use and execution of this profile, please refer to Jun et al. (2021). Elsevier 2021-12-16 /pmc/articles/PMC8689352/ /pubmed/34977685 http://dx.doi.org/10.1016/j.xpro.2021.101044 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Jun, Daniel
Zhang, Sylvester
Grzędowski, Adrian Jan
Mahey, Amita
Beatty, J. Thomas
Bizzotto, Dan
Purification and preparation of Rhodobacter sphaeroides reaction centers for photocurrent measurements and atomic force microscopy characterization
title Purification and preparation of Rhodobacter sphaeroides reaction centers for photocurrent measurements and atomic force microscopy characterization
title_full Purification and preparation of Rhodobacter sphaeroides reaction centers for photocurrent measurements and atomic force microscopy characterization
title_fullStr Purification and preparation of Rhodobacter sphaeroides reaction centers for photocurrent measurements and atomic force microscopy characterization
title_full_unstemmed Purification and preparation of Rhodobacter sphaeroides reaction centers for photocurrent measurements and atomic force microscopy characterization
title_short Purification and preparation of Rhodobacter sphaeroides reaction centers for photocurrent measurements and atomic force microscopy characterization
title_sort purification and preparation of rhodobacter sphaeroides reaction centers for photocurrent measurements and atomic force microscopy characterization
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8689352/
https://www.ncbi.nlm.nih.gov/pubmed/34977685
http://dx.doi.org/10.1016/j.xpro.2021.101044
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