MATISSE: An analysis protocol for combining imaging mass cytometry with fluorescence microscopy to generate single-cell data

Exploring tissue heterogeneity on a single-cell level by imaging mass cytometry (IMC) remains challenging because of its limiting resolution. We previously demonstrated that combining higher resolution fluorescence with IMC data in the analysis pipeline resulted in high-quality single-cell segmentat...

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Autores principales: Krijgsman, Daniëlle, Sinha, Neeraj, Baars, Matthijs J.D., van Dam, Stephanie, Amini, Mojtaba, Vercoulen, Yvonne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8689354/
https://www.ncbi.nlm.nih.gov/pubmed/34977680
http://dx.doi.org/10.1016/j.xpro.2021.101034
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author Krijgsman, Daniëlle
Sinha, Neeraj
Baars, Matthijs J.D.
van Dam, Stephanie
Amini, Mojtaba
Vercoulen, Yvonne
author_facet Krijgsman, Daniëlle
Sinha, Neeraj
Baars, Matthijs J.D.
van Dam, Stephanie
Amini, Mojtaba
Vercoulen, Yvonne
author_sort Krijgsman, Daniëlle
collection PubMed
description Exploring tissue heterogeneity on a single-cell level by imaging mass cytometry (IMC) remains challenging because of its limiting resolution. We previously demonstrated that combining higher resolution fluorescence with IMC data in the analysis pipeline resulted in high-quality single-cell segmentation. Here, we provide a step-by-step workflow of this MATISSE pipeline, including instructions regarding the staining procedure, and the analysis route to generate single-cell data. For complete details on the use and execution of this protocol, please refer to Baars et al., 2021.
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spelling pubmed-86893542021-12-30 MATISSE: An analysis protocol for combining imaging mass cytometry with fluorescence microscopy to generate single-cell data Krijgsman, Daniëlle Sinha, Neeraj Baars, Matthijs J.D. van Dam, Stephanie Amini, Mojtaba Vercoulen, Yvonne STAR Protoc Protocol Exploring tissue heterogeneity on a single-cell level by imaging mass cytometry (IMC) remains challenging because of its limiting resolution. We previously demonstrated that combining higher resolution fluorescence with IMC data in the analysis pipeline resulted in high-quality single-cell segmentation. Here, we provide a step-by-step workflow of this MATISSE pipeline, including instructions regarding the staining procedure, and the analysis route to generate single-cell data. For complete details on the use and execution of this protocol, please refer to Baars et al., 2021. Elsevier 2021-12-15 /pmc/articles/PMC8689354/ /pubmed/34977680 http://dx.doi.org/10.1016/j.xpro.2021.101034 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Krijgsman, Daniëlle
Sinha, Neeraj
Baars, Matthijs J.D.
van Dam, Stephanie
Amini, Mojtaba
Vercoulen, Yvonne
MATISSE: An analysis protocol for combining imaging mass cytometry with fluorescence microscopy to generate single-cell data
title MATISSE: An analysis protocol for combining imaging mass cytometry with fluorescence microscopy to generate single-cell data
title_full MATISSE: An analysis protocol for combining imaging mass cytometry with fluorescence microscopy to generate single-cell data
title_fullStr MATISSE: An analysis protocol for combining imaging mass cytometry with fluorescence microscopy to generate single-cell data
title_full_unstemmed MATISSE: An analysis protocol for combining imaging mass cytometry with fluorescence microscopy to generate single-cell data
title_short MATISSE: An analysis protocol for combining imaging mass cytometry with fluorescence microscopy to generate single-cell data
title_sort matisse: an analysis protocol for combining imaging mass cytometry with fluorescence microscopy to generate single-cell data
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8689354/
https://www.ncbi.nlm.nih.gov/pubmed/34977680
http://dx.doi.org/10.1016/j.xpro.2021.101034
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