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Intestinal organoid co-culture protocol to study cell competition in vitro

Intestinal organoid cultures are a powerful tool to study epithelial cells in vitro, as they are able to proliferate and differentiate into all cell lineages observed in vivo. Co-culturing organoids with distinct genetic backgrounds provides an excellent approach to study contact dependent and indep...

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Autores principales: van Neerven, Sanne M., Ramadan, Rana, van Driel, Milou S., Huels, David J., Vermeulen, Louis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8689363/
https://www.ncbi.nlm.nih.gov/pubmed/34977689
http://dx.doi.org/10.1016/j.xpro.2021.101050
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author van Neerven, Sanne M.
Ramadan, Rana
van Driel, Milou S.
Huels, David J.
Vermeulen, Louis
author_facet van Neerven, Sanne M.
Ramadan, Rana
van Driel, Milou S.
Huels, David J.
Vermeulen, Louis
author_sort van Neerven, Sanne M.
collection PubMed
description Intestinal organoid cultures are a powerful tool to study epithelial cells in vitro, as they are able to proliferate and differentiate into all cell lineages observed in vivo. Co-culturing organoids with distinct genetic backgrounds provides an excellent approach to study contact dependent and independent interactions between healthy and mutant epithelial intestinal cells. Here, we provide 2D and 3D approaches to mouse organoid co-cultures using fluorescently labeled organoids and demonstrate the analysis of these co-cultures using flow cytometry and microscopy-based approaches. For complete details on the use and execution of this profile, please refer to van Neerven et al., 2021.
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spelling pubmed-86893632021-12-30 Intestinal organoid co-culture protocol to study cell competition in vitro van Neerven, Sanne M. Ramadan, Rana van Driel, Milou S. Huels, David J. Vermeulen, Louis STAR Protoc Protocol Intestinal organoid cultures are a powerful tool to study epithelial cells in vitro, as they are able to proliferate and differentiate into all cell lineages observed in vivo. Co-culturing organoids with distinct genetic backgrounds provides an excellent approach to study contact dependent and independent interactions between healthy and mutant epithelial intestinal cells. Here, we provide 2D and 3D approaches to mouse organoid co-cultures using fluorescently labeled organoids and demonstrate the analysis of these co-cultures using flow cytometry and microscopy-based approaches. For complete details on the use and execution of this profile, please refer to van Neerven et al., 2021. Elsevier 2021-12-16 /pmc/articles/PMC8689363/ /pubmed/34977689 http://dx.doi.org/10.1016/j.xpro.2021.101050 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
van Neerven, Sanne M.
Ramadan, Rana
van Driel, Milou S.
Huels, David J.
Vermeulen, Louis
Intestinal organoid co-culture protocol to study cell competition in vitro
title Intestinal organoid co-culture protocol to study cell competition in vitro
title_full Intestinal organoid co-culture protocol to study cell competition in vitro
title_fullStr Intestinal organoid co-culture protocol to study cell competition in vitro
title_full_unstemmed Intestinal organoid co-culture protocol to study cell competition in vitro
title_short Intestinal organoid co-culture protocol to study cell competition in vitro
title_sort intestinal organoid co-culture protocol to study cell competition in vitro
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8689363/
https://www.ncbi.nlm.nih.gov/pubmed/34977689
http://dx.doi.org/10.1016/j.xpro.2021.101050
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