Flow cytometry analysis of the subpopulations of mouse keratinocytes and skin immune cells

Skin is our body’s outermost physical barrier and an immunological interface enriched with various immune and non-immune cells. However, efficient generation of single-cell suspensions for flow cytometry analysis can be challenging. Here, we provide protocols to obtain epidermal and whole skin cell...

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Detalles Bibliográficos
Autores principales: Sakamoto, Keiko, Goel, Shubham, Funakoshi, Atsuko, Honda, Tetsuya, Nagao, Keisuke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8689364/
https://www.ncbi.nlm.nih.gov/pubmed/34977690
http://dx.doi.org/10.1016/j.xpro.2021.101052
Descripción
Sumario:Skin is our body’s outermost physical barrier and an immunological interface enriched with various immune and non-immune cells. However, efficient generation of single-cell suspensions for flow cytometry analysis can be challenging. Here, we provide protocols to obtain epidermal and whole skin cell suspensions as well as gating strategies to identify mouse keratinocytes and skin immune cell subsets via flow cytometry. For complete details on the use and execution of this protocol, please refer to Sakamoto et al. (2021).

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