Flow cytometry analysis of the subpopulations of mouse keratinocytes and skin immune cells

Skin is our body’s outermost physical barrier and an immunological interface enriched with various immune and non-immune cells. However, efficient generation of single-cell suspensions for flow cytometry analysis can be challenging. Here, we provide protocols to obtain epidermal and whole skin cell...

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Autores principales: Sakamoto, Keiko, Goel, Shubham, Funakoshi, Atsuko, Honda, Tetsuya, Nagao, Keisuke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8689364/
https://www.ncbi.nlm.nih.gov/pubmed/34977690
http://dx.doi.org/10.1016/j.xpro.2021.101052
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author Sakamoto, Keiko
Goel, Shubham
Funakoshi, Atsuko
Honda, Tetsuya
Nagao, Keisuke
author_facet Sakamoto, Keiko
Goel, Shubham
Funakoshi, Atsuko
Honda, Tetsuya
Nagao, Keisuke
author_sort Sakamoto, Keiko
collection PubMed
description Skin is our body’s outermost physical barrier and an immunological interface enriched with various immune and non-immune cells. However, efficient generation of single-cell suspensions for flow cytometry analysis can be challenging. Here, we provide protocols to obtain epidermal and whole skin cell suspensions as well as gating strategies to identify mouse keratinocytes and skin immune cell subsets via flow cytometry. For complete details on the use and execution of this protocol, please refer to Sakamoto et al. (2021).
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spelling pubmed-86893642021-12-30 Flow cytometry analysis of the subpopulations of mouse keratinocytes and skin immune cells Sakamoto, Keiko Goel, Shubham Funakoshi, Atsuko Honda, Tetsuya Nagao, Keisuke STAR Protoc Protocol Skin is our body’s outermost physical barrier and an immunological interface enriched with various immune and non-immune cells. However, efficient generation of single-cell suspensions for flow cytometry analysis can be challenging. Here, we provide protocols to obtain epidermal and whole skin cell suspensions as well as gating strategies to identify mouse keratinocytes and skin immune cell subsets via flow cytometry. For complete details on the use and execution of this protocol, please refer to Sakamoto et al. (2021). Elsevier 2021-12-16 /pmc/articles/PMC8689364/ /pubmed/34977690 http://dx.doi.org/10.1016/j.xpro.2021.101052 Text en © 2021. https://creativecommons.org/licenses/by/3.0/igo/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/igo/).
spellingShingle Protocol
Sakamoto, Keiko
Goel, Shubham
Funakoshi, Atsuko
Honda, Tetsuya
Nagao, Keisuke
Flow cytometry analysis of the subpopulations of mouse keratinocytes and skin immune cells
title Flow cytometry analysis of the subpopulations of mouse keratinocytes and skin immune cells
title_full Flow cytometry analysis of the subpopulations of mouse keratinocytes and skin immune cells
title_fullStr Flow cytometry analysis of the subpopulations of mouse keratinocytes and skin immune cells
title_full_unstemmed Flow cytometry analysis of the subpopulations of mouse keratinocytes and skin immune cells
title_short Flow cytometry analysis of the subpopulations of mouse keratinocytes and skin immune cells
title_sort flow cytometry analysis of the subpopulations of mouse keratinocytes and skin immune cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8689364/
https://www.ncbi.nlm.nih.gov/pubmed/34977690
http://dx.doi.org/10.1016/j.xpro.2021.101052
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