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Single-Cell Time-Lapse Observation Reveals Cell Shrinkage upon Cell Death in Batch Culture of Saccharomyces cerevisiae
Saccharomyces cerevisiae is a model organism for aging and longevity studies. In a clonal population of S. cerevisiae, the timing of cell death in the stationary phase is not synchronized, indicating that heterogeneity exists in survival at a single-cell level. Heterogeneity also exists in the cell...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8689514/ https://www.ncbi.nlm.nih.gov/pubmed/34933452 http://dx.doi.org/10.1128/mBio.03094-21 |
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author | Kato, Setsu Suzuki, Kenta Kenjo, Taiki Kato, Junya Aoi, Yoshiteru Nakashimada, Yutaka |
author_facet | Kato, Setsu Suzuki, Kenta Kenjo, Taiki Kato, Junya Aoi, Yoshiteru Nakashimada, Yutaka |
author_sort | Kato, Setsu |
collection | PubMed |
description | Saccharomyces cerevisiae is a model organism for aging and longevity studies. In a clonal population of S. cerevisiae, the timing of cell death in the stationary phase is not synchronized, indicating that heterogeneity exists in survival at a single-cell level. Heterogeneity also exists in the cell size, and its correlation with the death rate has been discussed in past studies. However, the direct cause of the heterogeneity in survival remains unknown. In this report, we revisited this question and asked whether the death rate has any correlation with cell size. Past studies did not exclude a possibility that cells change their size upon or after death. If such a change exists, the size dependence of cell death could be misinterpreted. Therefore, we analyzed the correlation between the death rate and cell size before death by time-lapse imaging. It turned out that the size dependence of the death rate varied from one strain to another, suggesting that general principles between cell size and death do not exist. Instead, cells shrink upon cell death, resulting in the accumulation of small dead cells. The degree of cell shrinkage was proportional to the cell size, and the ratio was constant in two strains, which is between 25 and 28%, suggesting the presence of general principles and mechanisms behind the shrinkage event upon cell death. Further investigation of the cause and mechanism of the shrinkage will help us to understand the process of cell death and the origin of the heterogeneity in survival. |
format | Online Article Text |
id | pubmed-8689514 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-86895142021-12-27 Single-Cell Time-Lapse Observation Reveals Cell Shrinkage upon Cell Death in Batch Culture of Saccharomyces cerevisiae Kato, Setsu Suzuki, Kenta Kenjo, Taiki Kato, Junya Aoi, Yoshiteru Nakashimada, Yutaka mBio Observation Saccharomyces cerevisiae is a model organism for aging and longevity studies. In a clonal population of S. cerevisiae, the timing of cell death in the stationary phase is not synchronized, indicating that heterogeneity exists in survival at a single-cell level. Heterogeneity also exists in the cell size, and its correlation with the death rate has been discussed in past studies. However, the direct cause of the heterogeneity in survival remains unknown. In this report, we revisited this question and asked whether the death rate has any correlation with cell size. Past studies did not exclude a possibility that cells change their size upon or after death. If such a change exists, the size dependence of cell death could be misinterpreted. Therefore, we analyzed the correlation between the death rate and cell size before death by time-lapse imaging. It turned out that the size dependence of the death rate varied from one strain to another, suggesting that general principles between cell size and death do not exist. Instead, cells shrink upon cell death, resulting in the accumulation of small dead cells. The degree of cell shrinkage was proportional to the cell size, and the ratio was constant in two strains, which is between 25 and 28%, suggesting the presence of general principles and mechanisms behind the shrinkage event upon cell death. Further investigation of the cause and mechanism of the shrinkage will help us to understand the process of cell death and the origin of the heterogeneity in survival. American Society for Microbiology 2021-12-21 /pmc/articles/PMC8689514/ /pubmed/34933452 http://dx.doi.org/10.1128/mBio.03094-21 Text en Copyright © 2021 Kato et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Observation Kato, Setsu Suzuki, Kenta Kenjo, Taiki Kato, Junya Aoi, Yoshiteru Nakashimada, Yutaka Single-Cell Time-Lapse Observation Reveals Cell Shrinkage upon Cell Death in Batch Culture of Saccharomyces cerevisiae |
title | Single-Cell Time-Lapse Observation Reveals Cell Shrinkage upon Cell Death in Batch Culture of Saccharomyces cerevisiae |
title_full | Single-Cell Time-Lapse Observation Reveals Cell Shrinkage upon Cell Death in Batch Culture of Saccharomyces cerevisiae |
title_fullStr | Single-Cell Time-Lapse Observation Reveals Cell Shrinkage upon Cell Death in Batch Culture of Saccharomyces cerevisiae |
title_full_unstemmed | Single-Cell Time-Lapse Observation Reveals Cell Shrinkage upon Cell Death in Batch Culture of Saccharomyces cerevisiae |
title_short | Single-Cell Time-Lapse Observation Reveals Cell Shrinkage upon Cell Death in Batch Culture of Saccharomyces cerevisiae |
title_sort | single-cell time-lapse observation reveals cell shrinkage upon cell death in batch culture of saccharomyces cerevisiae |
topic | Observation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8689514/ https://www.ncbi.nlm.nih.gov/pubmed/34933452 http://dx.doi.org/10.1128/mBio.03094-21 |
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