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Single-Cell Time-Lapse Observation Reveals Cell Shrinkage upon Cell Death in Batch Culture of Saccharomyces cerevisiae

Saccharomyces cerevisiae is a model organism for aging and longevity studies. In a clonal population of S. cerevisiae, the timing of cell death in the stationary phase is not synchronized, indicating that heterogeneity exists in survival at a single-cell level. Heterogeneity also exists in the cell...

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Autores principales: Kato, Setsu, Suzuki, Kenta, Kenjo, Taiki, Kato, Junya, Aoi, Yoshiteru, Nakashimada, Yutaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8689514/
https://www.ncbi.nlm.nih.gov/pubmed/34933452
http://dx.doi.org/10.1128/mBio.03094-21
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author Kato, Setsu
Suzuki, Kenta
Kenjo, Taiki
Kato, Junya
Aoi, Yoshiteru
Nakashimada, Yutaka
author_facet Kato, Setsu
Suzuki, Kenta
Kenjo, Taiki
Kato, Junya
Aoi, Yoshiteru
Nakashimada, Yutaka
author_sort Kato, Setsu
collection PubMed
description Saccharomyces cerevisiae is a model organism for aging and longevity studies. In a clonal population of S. cerevisiae, the timing of cell death in the stationary phase is not synchronized, indicating that heterogeneity exists in survival at a single-cell level. Heterogeneity also exists in the cell size, and its correlation with the death rate has been discussed in past studies. However, the direct cause of the heterogeneity in survival remains unknown. In this report, we revisited this question and asked whether the death rate has any correlation with cell size. Past studies did not exclude a possibility that cells change their size upon or after death. If such a change exists, the size dependence of cell death could be misinterpreted. Therefore, we analyzed the correlation between the death rate and cell size before death by time-lapse imaging. It turned out that the size dependence of the death rate varied from one strain to another, suggesting that general principles between cell size and death do not exist. Instead, cells shrink upon cell death, resulting in the accumulation of small dead cells. The degree of cell shrinkage was proportional to the cell size, and the ratio was constant in two strains, which is between 25 and 28%, suggesting the presence of general principles and mechanisms behind the shrinkage event upon cell death. Further investigation of the cause and mechanism of the shrinkage will help us to understand the process of cell death and the origin of the heterogeneity in survival.
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spelling pubmed-86895142021-12-27 Single-Cell Time-Lapse Observation Reveals Cell Shrinkage upon Cell Death in Batch Culture of Saccharomyces cerevisiae Kato, Setsu Suzuki, Kenta Kenjo, Taiki Kato, Junya Aoi, Yoshiteru Nakashimada, Yutaka mBio Observation Saccharomyces cerevisiae is a model organism for aging and longevity studies. In a clonal population of S. cerevisiae, the timing of cell death in the stationary phase is not synchronized, indicating that heterogeneity exists in survival at a single-cell level. Heterogeneity also exists in the cell size, and its correlation with the death rate has been discussed in past studies. However, the direct cause of the heterogeneity in survival remains unknown. In this report, we revisited this question and asked whether the death rate has any correlation with cell size. Past studies did not exclude a possibility that cells change their size upon or after death. If such a change exists, the size dependence of cell death could be misinterpreted. Therefore, we analyzed the correlation between the death rate and cell size before death by time-lapse imaging. It turned out that the size dependence of the death rate varied from one strain to another, suggesting that general principles between cell size and death do not exist. Instead, cells shrink upon cell death, resulting in the accumulation of small dead cells. The degree of cell shrinkage was proportional to the cell size, and the ratio was constant in two strains, which is between 25 and 28%, suggesting the presence of general principles and mechanisms behind the shrinkage event upon cell death. Further investigation of the cause and mechanism of the shrinkage will help us to understand the process of cell death and the origin of the heterogeneity in survival. American Society for Microbiology 2021-12-21 /pmc/articles/PMC8689514/ /pubmed/34933452 http://dx.doi.org/10.1128/mBio.03094-21 Text en Copyright © 2021 Kato et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Observation
Kato, Setsu
Suzuki, Kenta
Kenjo, Taiki
Kato, Junya
Aoi, Yoshiteru
Nakashimada, Yutaka
Single-Cell Time-Lapse Observation Reveals Cell Shrinkage upon Cell Death in Batch Culture of Saccharomyces cerevisiae
title Single-Cell Time-Lapse Observation Reveals Cell Shrinkage upon Cell Death in Batch Culture of Saccharomyces cerevisiae
title_full Single-Cell Time-Lapse Observation Reveals Cell Shrinkage upon Cell Death in Batch Culture of Saccharomyces cerevisiae
title_fullStr Single-Cell Time-Lapse Observation Reveals Cell Shrinkage upon Cell Death in Batch Culture of Saccharomyces cerevisiae
title_full_unstemmed Single-Cell Time-Lapse Observation Reveals Cell Shrinkage upon Cell Death in Batch Culture of Saccharomyces cerevisiae
title_short Single-Cell Time-Lapse Observation Reveals Cell Shrinkage upon Cell Death in Batch Culture of Saccharomyces cerevisiae
title_sort single-cell time-lapse observation reveals cell shrinkage upon cell death in batch culture of saccharomyces cerevisiae
topic Observation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8689514/
https://www.ncbi.nlm.nih.gov/pubmed/34933452
http://dx.doi.org/10.1128/mBio.03094-21
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