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Tools for Decoding Ubiquitin Signaling in DNA Repair

The maintenance of genome stability requires dedicated DNA repair processes and pathways that are essential for the faithful duplication and propagation of chromosomes. These DNA repair mechanisms counteract the potentially deleterious impact of the frequent genotoxic challenges faced by cells from...

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Detalles Bibliográficos
Autores principales: Foster, Benjamin, Attwood, Martin, Gibbs-Seymour, Ian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8690248/
https://www.ncbi.nlm.nih.gov/pubmed/34950659
http://dx.doi.org/10.3389/fcell.2021.760226
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author Foster, Benjamin
Attwood, Martin
Gibbs-Seymour, Ian
author_facet Foster, Benjamin
Attwood, Martin
Gibbs-Seymour, Ian
author_sort Foster, Benjamin
collection PubMed
description The maintenance of genome stability requires dedicated DNA repair processes and pathways that are essential for the faithful duplication and propagation of chromosomes. These DNA repair mechanisms counteract the potentially deleterious impact of the frequent genotoxic challenges faced by cells from both exogenous and endogenous agents. Intrinsic to these mechanisms, cells have an arsenal of protein factors that can be utilised to promote repair processes in response to DNA lesions. Orchestration of the protein factors within the various cellular DNA repair pathways is performed, in part, by post-translational modifications, such as phosphorylation, ubiquitin, SUMO and other ubiquitin-like modifiers (UBLs). In this review, we firstly explore recent advances in the tools for identifying factors involved in both DNA repair and ubiquitin signaling pathways. We then expand on this by evaluating the growing repertoire of proteomic, biochemical and structural techniques available to further understand the mechanistic basis by which these complex modifications regulate DNA repair. Together, we provide a snapshot of the range of methods now available to investigate and decode how ubiquitin signaling can promote DNA repair and maintain genome stability in mammalian cells.
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spelling pubmed-86902482021-12-22 Tools for Decoding Ubiquitin Signaling in DNA Repair Foster, Benjamin Attwood, Martin Gibbs-Seymour, Ian Front Cell Dev Biol Cell and Developmental Biology The maintenance of genome stability requires dedicated DNA repair processes and pathways that are essential for the faithful duplication and propagation of chromosomes. These DNA repair mechanisms counteract the potentially deleterious impact of the frequent genotoxic challenges faced by cells from both exogenous and endogenous agents. Intrinsic to these mechanisms, cells have an arsenal of protein factors that can be utilised to promote repair processes in response to DNA lesions. Orchestration of the protein factors within the various cellular DNA repair pathways is performed, in part, by post-translational modifications, such as phosphorylation, ubiquitin, SUMO and other ubiquitin-like modifiers (UBLs). In this review, we firstly explore recent advances in the tools for identifying factors involved in both DNA repair and ubiquitin signaling pathways. We then expand on this by evaluating the growing repertoire of proteomic, biochemical and structural techniques available to further understand the mechanistic basis by which these complex modifications regulate DNA repair. Together, we provide a snapshot of the range of methods now available to investigate and decode how ubiquitin signaling can promote DNA repair and maintain genome stability in mammalian cells. Frontiers Media S.A. 2021-12-07 /pmc/articles/PMC8690248/ /pubmed/34950659 http://dx.doi.org/10.3389/fcell.2021.760226 Text en Copyright © 2021 Foster, Attwood and Gibbs-Seymour. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Foster, Benjamin
Attwood, Martin
Gibbs-Seymour, Ian
Tools for Decoding Ubiquitin Signaling in DNA Repair
title Tools for Decoding Ubiquitin Signaling in DNA Repair
title_full Tools for Decoding Ubiquitin Signaling in DNA Repair
title_fullStr Tools for Decoding Ubiquitin Signaling in DNA Repair
title_full_unstemmed Tools for Decoding Ubiquitin Signaling in DNA Repair
title_short Tools for Decoding Ubiquitin Signaling in DNA Repair
title_sort tools for decoding ubiquitin signaling in dna repair
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8690248/
https://www.ncbi.nlm.nih.gov/pubmed/34950659
http://dx.doi.org/10.3389/fcell.2021.760226
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