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Lipidomics investigations into the tissue phospholipidomic landscape of invasive ductal carcinoma of the breast

The need of identifying alternative therapeutic targets for invasive ductal carcinoma (IDC) of the breast with high specificity and sensitivity for effective therapeutic intervention is crucial for lowering the risk of fatality. Lipidomics has emerged as a key area for the discovery of potential can...

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Autores principales: Taware, Ravindra, More, Tushar H., Bagadi, Muralidhararao, Taunk, Khushman, Mane, Anupama, Rapole, Srikanth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8690848/
https://www.ncbi.nlm.nih.gov/pubmed/35423059
http://dx.doi.org/10.1039/d0ra07368g
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author Taware, Ravindra
More, Tushar H.
Bagadi, Muralidhararao
Taunk, Khushman
Mane, Anupama
Rapole, Srikanth
author_facet Taware, Ravindra
More, Tushar H.
Bagadi, Muralidhararao
Taunk, Khushman
Mane, Anupama
Rapole, Srikanth
author_sort Taware, Ravindra
collection PubMed
description The need of identifying alternative therapeutic targets for invasive ductal carcinoma (IDC) of the breast with high specificity and sensitivity for effective therapeutic intervention is crucial for lowering the risk of fatality. Lipidomics has emerged as a key area for the discovery of potential candidates owing to its several shared pathways between cancer cell proliferation and survival. In the current study, we performed comparative phospholipidomic analysis of IDC, benign and control tissue samples of the breast to identify the significant lipid alterations associated with malignant transformation. A total of 33 each age-matched tissue samples from malignant, benign and control were analyzed to identify the altered phospholipids by using liquid chromatography-multiple reaction monitoring mass spectrometry (LC-MRM/MS). A combination of univariate and multivariate statistical approaches was used to select the phospholipid species with the highest contribution in group segregation. Furthermore, these altered phospholipids were structurally confirmed by tandem mass spectrometry. A total of 244 phospholipids were detected consistently at quantifiable levels, out of which 32 were significantly altered in IDC of the breast. Moreover, in pairwise comparison of IDC against benign and control samples, 11 phospholipids were found to be significantly differentially expressed. Particularly, LPI 20:3, PE (22:1/22:2), LPE 20:0 and PC (20:4/22:4) were observed to be most significantly associated with IDC tissue samples. Apart from that, we also identified that long-chain unsaturated fatty acids were enriched in the IDC tissue samples as compared to benign and control samples, indicating its possible association with the invasive phenotype.
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spelling pubmed-86908482022-04-13 Lipidomics investigations into the tissue phospholipidomic landscape of invasive ductal carcinoma of the breast Taware, Ravindra More, Tushar H. Bagadi, Muralidhararao Taunk, Khushman Mane, Anupama Rapole, Srikanth RSC Adv Chemistry The need of identifying alternative therapeutic targets for invasive ductal carcinoma (IDC) of the breast with high specificity and sensitivity for effective therapeutic intervention is crucial for lowering the risk of fatality. Lipidomics has emerged as a key area for the discovery of potential candidates owing to its several shared pathways between cancer cell proliferation and survival. In the current study, we performed comparative phospholipidomic analysis of IDC, benign and control tissue samples of the breast to identify the significant lipid alterations associated with malignant transformation. A total of 33 each age-matched tissue samples from malignant, benign and control were analyzed to identify the altered phospholipids by using liquid chromatography-multiple reaction monitoring mass spectrometry (LC-MRM/MS). A combination of univariate and multivariate statistical approaches was used to select the phospholipid species with the highest contribution in group segregation. Furthermore, these altered phospholipids were structurally confirmed by tandem mass spectrometry. A total of 244 phospholipids were detected consistently at quantifiable levels, out of which 32 were significantly altered in IDC of the breast. Moreover, in pairwise comparison of IDC against benign and control samples, 11 phospholipids were found to be significantly differentially expressed. Particularly, LPI 20:3, PE (22:1/22:2), LPE 20:0 and PC (20:4/22:4) were observed to be most significantly associated with IDC tissue samples. Apart from that, we also identified that long-chain unsaturated fatty acids were enriched in the IDC tissue samples as compared to benign and control samples, indicating its possible association with the invasive phenotype. The Royal Society of Chemistry 2020-12-22 /pmc/articles/PMC8690848/ /pubmed/35423059 http://dx.doi.org/10.1039/d0ra07368g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Taware, Ravindra
More, Tushar H.
Bagadi, Muralidhararao
Taunk, Khushman
Mane, Anupama
Rapole, Srikanth
Lipidomics investigations into the tissue phospholipidomic landscape of invasive ductal carcinoma of the breast
title Lipidomics investigations into the tissue phospholipidomic landscape of invasive ductal carcinoma of the breast
title_full Lipidomics investigations into the tissue phospholipidomic landscape of invasive ductal carcinoma of the breast
title_fullStr Lipidomics investigations into the tissue phospholipidomic landscape of invasive ductal carcinoma of the breast
title_full_unstemmed Lipidomics investigations into the tissue phospholipidomic landscape of invasive ductal carcinoma of the breast
title_short Lipidomics investigations into the tissue phospholipidomic landscape of invasive ductal carcinoma of the breast
title_sort lipidomics investigations into the tissue phospholipidomic landscape of invasive ductal carcinoma of the breast
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8690848/
https://www.ncbi.nlm.nih.gov/pubmed/35423059
http://dx.doi.org/10.1039/d0ra07368g
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