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Reference ranges of T lymphocyte subsets by single-platform among healthy population in southwest China

BACKGROUND: Appropriate reference ranges of T lymphocyte subsets are essential for immune status evaluation of patients with immunological diseases. We aim to establish the age- and sex-related reference intervals of T lymphocyte subsets by single-platform for the southwest China population using th...

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Autores principales: Wei, Bin, Guo, Ying, Zhang, Liangjun, Zhong, Huixiu, Miao, Qiang, Yan, Lin, Bai, Yangjuan, Feng, Weihua, Liu, Weiping, Niu, Qian, Li, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8690880/
https://www.ncbi.nlm.nih.gov/pubmed/34930155
http://dx.doi.org/10.1186/s12865-021-00474-0
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author Wei, Bin
Guo, Ying
Zhang, Liangjun
Zhong, Huixiu
Miao, Qiang
Yan, Lin
Bai, Yangjuan
Feng, Weihua
Liu, Weiping
Niu, Qian
Li, Yi
author_facet Wei, Bin
Guo, Ying
Zhang, Liangjun
Zhong, Huixiu
Miao, Qiang
Yan, Lin
Bai, Yangjuan
Feng, Weihua
Liu, Weiping
Niu, Qian
Li, Yi
author_sort Wei, Bin
collection PubMed
description BACKGROUND: Appropriate reference ranges of T lymphocyte subsets are essential for immune status evaluation of patients with immunological diseases. We aim to establish the age- and sex-related reference intervals of T lymphocyte subsets by single-platform for the southwest China population using the indirect method with the data resulting from 53,822 cases of periodic health examination individuals in the Laboratory Information System (LIS) of West China Hospital from 2018 to 2020. METHODS: We used the Box-Cox conversion combined with the Tukey method to normalize the data and eliminate the outliers, and the nonparametric method to estimate the 95% distribution reference intervals. RESULTS: We initially established the reference ranges of T lymphocyte subsets by single-platform among healthy population in southwest China by indirect method (See text for details). Using the standard normal deviate test (z-test) suggested by Harris and Boyd according to CLSI EP28-A3C, which is more scientific, we found the reference ranges of T lymphocyte subsets should be differentiated by ages and genders since the reference ranges of T lymphocyte subsets by single-platform in different ages and genders are significantly different. CONCLUSIONS: We further demonstrated the absolute count of CD3 + T cell, CD3 + CD4 + T cell, CD3 + CD8 + T cell decreased with aging, which is more marked in men and CD3 + CD8 + T cell count, and the obtained reference intervals were superior to the reference intervals derived from the reagent specification currently in use. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12865-021-00474-0.
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spelling pubmed-86908802021-12-21 Reference ranges of T lymphocyte subsets by single-platform among healthy population in southwest China Wei, Bin Guo, Ying Zhang, Liangjun Zhong, Huixiu Miao, Qiang Yan, Lin Bai, Yangjuan Feng, Weihua Liu, Weiping Niu, Qian Li, Yi BMC Immunol Research BACKGROUND: Appropriate reference ranges of T lymphocyte subsets are essential for immune status evaluation of patients with immunological diseases. We aim to establish the age- and sex-related reference intervals of T lymphocyte subsets by single-platform for the southwest China population using the indirect method with the data resulting from 53,822 cases of periodic health examination individuals in the Laboratory Information System (LIS) of West China Hospital from 2018 to 2020. METHODS: We used the Box-Cox conversion combined with the Tukey method to normalize the data and eliminate the outliers, and the nonparametric method to estimate the 95% distribution reference intervals. RESULTS: We initially established the reference ranges of T lymphocyte subsets by single-platform among healthy population in southwest China by indirect method (See text for details). Using the standard normal deviate test (z-test) suggested by Harris and Boyd according to CLSI EP28-A3C, which is more scientific, we found the reference ranges of T lymphocyte subsets should be differentiated by ages and genders since the reference ranges of T lymphocyte subsets by single-platform in different ages and genders are significantly different. CONCLUSIONS: We further demonstrated the absolute count of CD3 + T cell, CD3 + CD4 + T cell, CD3 + CD8 + T cell decreased with aging, which is more marked in men and CD3 + CD8 + T cell count, and the obtained reference intervals were superior to the reference intervals derived from the reagent specification currently in use. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12865-021-00474-0. BioMed Central 2021-12-20 /pmc/articles/PMC8690880/ /pubmed/34930155 http://dx.doi.org/10.1186/s12865-021-00474-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Wei, Bin
Guo, Ying
Zhang, Liangjun
Zhong, Huixiu
Miao, Qiang
Yan, Lin
Bai, Yangjuan
Feng, Weihua
Liu, Weiping
Niu, Qian
Li, Yi
Reference ranges of T lymphocyte subsets by single-platform among healthy population in southwest China
title Reference ranges of T lymphocyte subsets by single-platform among healthy population in southwest China
title_full Reference ranges of T lymphocyte subsets by single-platform among healthy population in southwest China
title_fullStr Reference ranges of T lymphocyte subsets by single-platform among healthy population in southwest China
title_full_unstemmed Reference ranges of T lymphocyte subsets by single-platform among healthy population in southwest China
title_short Reference ranges of T lymphocyte subsets by single-platform among healthy population in southwest China
title_sort reference ranges of t lymphocyte subsets by single-platform among healthy population in southwest china
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8690880/
https://www.ncbi.nlm.nih.gov/pubmed/34930155
http://dx.doi.org/10.1186/s12865-021-00474-0
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