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Development of a sensitive competitive enzyme-linked immunosorbent assay for serodiagnosis of Burkholderia mallei, a Tier 1 select agent

Glanders is a highly contagious and potentially serious disease caused by Burkholderia mallei, a Tier 1 select agent. In this study, we raised a monoclonal antibody (mAb) against the lipopolysaccharide (LPS) of B. mallei and developed a competitive enzyme-linked immunosorbent assay (cELISA) for B. m...

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Autores principales: Wernery, Ulrich, Chan, Elaine, Raghavan, Rekha, Teng, Jade L. L., Syriac, Ginu, Siu, Sing-Yung, Joseph, Marina, Yeung, Man-Lung, Jia, Lilong, Cai, Jian-Piao, Chiu, Tsz-Ho, Lau, Susanna K. P., Woo, Patrick C. Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8691619/
https://www.ncbi.nlm.nih.gov/pubmed/34932554
http://dx.doi.org/10.1371/journal.pntd.0010007
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author Wernery, Ulrich
Chan, Elaine
Raghavan, Rekha
Teng, Jade L. L.
Syriac, Ginu
Siu, Sing-Yung
Joseph, Marina
Yeung, Man-Lung
Jia, Lilong
Cai, Jian-Piao
Chiu, Tsz-Ho
Lau, Susanna K. P.
Woo, Patrick C. Y.
author_facet Wernery, Ulrich
Chan, Elaine
Raghavan, Rekha
Teng, Jade L. L.
Syriac, Ginu
Siu, Sing-Yung
Joseph, Marina
Yeung, Man-Lung
Jia, Lilong
Cai, Jian-Piao
Chiu, Tsz-Ho
Lau, Susanna K. P.
Woo, Patrick C. Y.
author_sort Wernery, Ulrich
collection PubMed
description Glanders is a highly contagious and potentially serious disease caused by Burkholderia mallei, a Tier 1 select agent. In this study, we raised a monoclonal antibody (mAb) against the lipopolysaccharide (LPS) of B. mallei and developed a competitive enzyme-linked immunosorbent assay (cELISA) for B. mallei infection. Using the titrated optimal conditions of B. mallei-LPS (2 ng) for microtiter plate coating, sample serum dilution at 1:20 and 3.5 ng/μL anti-LPS mAb B5, the cutoff value of the cELISA was determined using serum samples from 136 glanders-free seronegative horses in Hong Kong. All calculated percentage inhibition (PI) values from these seronegative samples were below 39.6% inhibition (1.5 standard deviations above mean PI) and was used as the cutoff value. The diagnostic sensitivity of the developed LPS-based cELISA was first evaluated using sera from donkeys and mice inoculated with B. mallei. An increasing trend of PI values above the defined cELISA cutoff observed in the donkey and mouse sera suggested positive detection of anti-LPS antibodies. The sensitivity and specificity of the LPS-based cELISA was further evaluated using 31 serologically positive horse sera from glanders outbreaks in Bahrain and Kuwait, of which 30 were tested positive by the cELISA; and 21 seronegative horse sera and 20 seronegative donkey sera from Dubai, of which all were tested negative by the cELISA. A cELISA with high sensitivity (97.2%) and specificity (100%) for the detection of B. mallei antibodies in different animals was developed.
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spelling pubmed-86916192021-12-22 Development of a sensitive competitive enzyme-linked immunosorbent assay for serodiagnosis of Burkholderia mallei, a Tier 1 select agent Wernery, Ulrich Chan, Elaine Raghavan, Rekha Teng, Jade L. L. Syriac, Ginu Siu, Sing-Yung Joseph, Marina Yeung, Man-Lung Jia, Lilong Cai, Jian-Piao Chiu, Tsz-Ho Lau, Susanna K. P. Woo, Patrick C. Y. PLoS Negl Trop Dis Research Article Glanders is a highly contagious and potentially serious disease caused by Burkholderia mallei, a Tier 1 select agent. In this study, we raised a monoclonal antibody (mAb) against the lipopolysaccharide (LPS) of B. mallei and developed a competitive enzyme-linked immunosorbent assay (cELISA) for B. mallei infection. Using the titrated optimal conditions of B. mallei-LPS (2 ng) for microtiter plate coating, sample serum dilution at 1:20 and 3.5 ng/μL anti-LPS mAb B5, the cutoff value of the cELISA was determined using serum samples from 136 glanders-free seronegative horses in Hong Kong. All calculated percentage inhibition (PI) values from these seronegative samples were below 39.6% inhibition (1.5 standard deviations above mean PI) and was used as the cutoff value. The diagnostic sensitivity of the developed LPS-based cELISA was first evaluated using sera from donkeys and mice inoculated with B. mallei. An increasing trend of PI values above the defined cELISA cutoff observed in the donkey and mouse sera suggested positive detection of anti-LPS antibodies. The sensitivity and specificity of the LPS-based cELISA was further evaluated using 31 serologically positive horse sera from glanders outbreaks in Bahrain and Kuwait, of which 30 were tested positive by the cELISA; and 21 seronegative horse sera and 20 seronegative donkey sera from Dubai, of which all were tested negative by the cELISA. A cELISA with high sensitivity (97.2%) and specificity (100%) for the detection of B. mallei antibodies in different animals was developed. Public Library of Science 2021-12-21 /pmc/articles/PMC8691619/ /pubmed/34932554 http://dx.doi.org/10.1371/journal.pntd.0010007 Text en © 2021 Wernery et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wernery, Ulrich
Chan, Elaine
Raghavan, Rekha
Teng, Jade L. L.
Syriac, Ginu
Siu, Sing-Yung
Joseph, Marina
Yeung, Man-Lung
Jia, Lilong
Cai, Jian-Piao
Chiu, Tsz-Ho
Lau, Susanna K. P.
Woo, Patrick C. Y.
Development of a sensitive competitive enzyme-linked immunosorbent assay for serodiagnosis of Burkholderia mallei, a Tier 1 select agent
title Development of a sensitive competitive enzyme-linked immunosorbent assay for serodiagnosis of Burkholderia mallei, a Tier 1 select agent
title_full Development of a sensitive competitive enzyme-linked immunosorbent assay for serodiagnosis of Burkholderia mallei, a Tier 1 select agent
title_fullStr Development of a sensitive competitive enzyme-linked immunosorbent assay for serodiagnosis of Burkholderia mallei, a Tier 1 select agent
title_full_unstemmed Development of a sensitive competitive enzyme-linked immunosorbent assay for serodiagnosis of Burkholderia mallei, a Tier 1 select agent
title_short Development of a sensitive competitive enzyme-linked immunosorbent assay for serodiagnosis of Burkholderia mallei, a Tier 1 select agent
title_sort development of a sensitive competitive enzyme-linked immunosorbent assay for serodiagnosis of burkholderia mallei, a tier 1 select agent
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8691619/
https://www.ncbi.nlm.nih.gov/pubmed/34932554
http://dx.doi.org/10.1371/journal.pntd.0010007
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