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Concerted dispersion of Staphylococcus aureus biofilm by bacteriophage and ‘green synthesized’ silver nanoparticles
Staphylococcal biofilms predominantly cause persistent nosocomial infections. The widespread antibiotic resistance followed by its ability to form biofilm in biological and inert surfaces often contributes to major complications in patients and veterinary animals. Strategic importance of bacteriopha...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8693614/ https://www.ncbi.nlm.nih.gov/pubmed/35424119 http://dx.doi.org/10.1039/d0ra09725j |
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author | Manoharadas, Salim Altaf, Mohammad Alrefaei, Abdulwahed Fahad Devasia, Rajesh Mamkulatil Badjah Hadj, Ahmed Yacine M. Abuhasil, Mohammed Saeed Ali |
author_facet | Manoharadas, Salim Altaf, Mohammad Alrefaei, Abdulwahed Fahad Devasia, Rajesh Mamkulatil Badjah Hadj, Ahmed Yacine M. Abuhasil, Mohammed Saeed Ali |
author_sort | Manoharadas, Salim |
collection | PubMed |
description | Staphylococcal biofilms predominantly cause persistent nosocomial infections. The widespread antibiotic resistance followed by its ability to form biofilm in biological and inert surfaces often contributes to major complications in patients and veterinary animals. Strategic importance of bacteriophage therapy against critical staphylococcal infections had been predicted ever since the advent of antibiotic resistant staphylococcal strains. The significance of metal nanoparticles in quenching biofilm associated bacteria was previously reported. In this study, we demonstrate a concerted action of ‘green synthesized’ silver nanoparticles and bacteriophages in removing pre-formed Staphylococcus aureus biofilms from an inert glass surface in a time dependent manner. Our results demonstrate, for the first time, the rapid co-operative dispersion of the bacterial biofilm. In addition, the synergistic activity of the nanoparticles and bacteriophages causes the loss of viability of the biofilm entrapped bacterial cells thus preventing establishment of a new infection and subsequent colonization. This work further opens up a platform for the combinational therapeutic approach with a variety of nanoparticles and bacteriophages against mono or poly bacterial biofilm in environmental, industrial or clinical settings. |
format | Online Article Text |
id | pubmed-8693614 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-86936142022-04-13 Concerted dispersion of Staphylococcus aureus biofilm by bacteriophage and ‘green synthesized’ silver nanoparticles Manoharadas, Salim Altaf, Mohammad Alrefaei, Abdulwahed Fahad Devasia, Rajesh Mamkulatil Badjah Hadj, Ahmed Yacine M. Abuhasil, Mohammed Saeed Ali RSC Adv Chemistry Staphylococcal biofilms predominantly cause persistent nosocomial infections. The widespread antibiotic resistance followed by its ability to form biofilm in biological and inert surfaces often contributes to major complications in patients and veterinary animals. Strategic importance of bacteriophage therapy against critical staphylococcal infections had been predicted ever since the advent of antibiotic resistant staphylococcal strains. The significance of metal nanoparticles in quenching biofilm associated bacteria was previously reported. In this study, we demonstrate a concerted action of ‘green synthesized’ silver nanoparticles and bacteriophages in removing pre-formed Staphylococcus aureus biofilms from an inert glass surface in a time dependent manner. Our results demonstrate, for the first time, the rapid co-operative dispersion of the bacterial biofilm. In addition, the synergistic activity of the nanoparticles and bacteriophages causes the loss of viability of the biofilm entrapped bacterial cells thus preventing establishment of a new infection and subsequent colonization. This work further opens up a platform for the combinational therapeutic approach with a variety of nanoparticles and bacteriophages against mono or poly bacterial biofilm in environmental, industrial or clinical settings. The Royal Society of Chemistry 2021-01-05 /pmc/articles/PMC8693614/ /pubmed/35424119 http://dx.doi.org/10.1039/d0ra09725j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Manoharadas, Salim Altaf, Mohammad Alrefaei, Abdulwahed Fahad Devasia, Rajesh Mamkulatil Badjah Hadj, Ahmed Yacine M. Abuhasil, Mohammed Saeed Ali Concerted dispersion of Staphylococcus aureus biofilm by bacteriophage and ‘green synthesized’ silver nanoparticles |
title | Concerted dispersion of Staphylococcus aureus biofilm by bacteriophage and ‘green synthesized’ silver nanoparticles |
title_full | Concerted dispersion of Staphylococcus aureus biofilm by bacteriophage and ‘green synthesized’ silver nanoparticles |
title_fullStr | Concerted dispersion of Staphylococcus aureus biofilm by bacteriophage and ‘green synthesized’ silver nanoparticles |
title_full_unstemmed | Concerted dispersion of Staphylococcus aureus biofilm by bacteriophage and ‘green synthesized’ silver nanoparticles |
title_short | Concerted dispersion of Staphylococcus aureus biofilm by bacteriophage and ‘green synthesized’ silver nanoparticles |
title_sort | concerted dispersion of staphylococcus aureus biofilm by bacteriophage and ‘green synthesized’ silver nanoparticles |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8693614/ https://www.ncbi.nlm.nih.gov/pubmed/35424119 http://dx.doi.org/10.1039/d0ra09725j |
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