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CD62L expression level determines the cell fate of myeloid progenitors
Hematopoietic cells differentiate through several progenitors in a hierarchical manner, and recent single-cell analyses have revealed substantial heterogeneity within each progenitor. Although common myeloid progenitors (CMPs) are defined as a multipotent cell population that can differentiate into...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8693656/ https://www.ncbi.nlm.nih.gov/pubmed/34798065 http://dx.doi.org/10.1016/j.stemcr.2021.10.012 |
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author | Ito, Yusuke Nakahara, Fumio Kagoya, Yuki Kurokawa, Mineo |
author_facet | Ito, Yusuke Nakahara, Fumio Kagoya, Yuki Kurokawa, Mineo |
author_sort | Ito, Yusuke |
collection | PubMed |
description | Hematopoietic cells differentiate through several progenitors in a hierarchical manner, and recent single-cell analyses have revealed substantial heterogeneity within each progenitor. Although common myeloid progenitors (CMPs) are defined as a multipotent cell population that can differentiate into granulocyte-monocyte progenitors (GMPs) and megakaryocyte-erythrocyte progenitors (MEPs), and GMPs generate neutrophils and monocytes, these myeloid progenitors must contain some lineage-committed progenitors. Through gene expression analysis at single-cell levels, we identified CD62L as a marker to reveal the heterogeneity. We confirmed that CD62L-negative CMPs represent “bona fide” CMPs, whereas CD62L-high CMPs are mostly restricted to GMP potentials both in mice and humans. In addition, we identified CD62L-negative GMPs as the most immature subsets in GMPs and Ly6C(+)CD62L-intermediate and Ly6C(+)CD62L-high GMPs are skewed to neutrophil and monocyte differentiation in mice, respectively. Our findings contribute to more profound understanding about the mechanism of myeloid differentiation. |
format | Online Article Text |
id | pubmed-8693656 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-86936562022-01-04 CD62L expression level determines the cell fate of myeloid progenitors Ito, Yusuke Nakahara, Fumio Kagoya, Yuki Kurokawa, Mineo Stem Cell Reports Article Hematopoietic cells differentiate through several progenitors in a hierarchical manner, and recent single-cell analyses have revealed substantial heterogeneity within each progenitor. Although common myeloid progenitors (CMPs) are defined as a multipotent cell population that can differentiate into granulocyte-monocyte progenitors (GMPs) and megakaryocyte-erythrocyte progenitors (MEPs), and GMPs generate neutrophils and monocytes, these myeloid progenitors must contain some lineage-committed progenitors. Through gene expression analysis at single-cell levels, we identified CD62L as a marker to reveal the heterogeneity. We confirmed that CD62L-negative CMPs represent “bona fide” CMPs, whereas CD62L-high CMPs are mostly restricted to GMP potentials both in mice and humans. In addition, we identified CD62L-negative GMPs as the most immature subsets in GMPs and Ly6C(+)CD62L-intermediate and Ly6C(+)CD62L-high GMPs are skewed to neutrophil and monocyte differentiation in mice, respectively. Our findings contribute to more profound understanding about the mechanism of myeloid differentiation. Elsevier 2021-11-18 /pmc/articles/PMC8693656/ /pubmed/34798065 http://dx.doi.org/10.1016/j.stemcr.2021.10.012 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ito, Yusuke Nakahara, Fumio Kagoya, Yuki Kurokawa, Mineo CD62L expression level determines the cell fate of myeloid progenitors |
title | CD62L expression level determines the cell fate of myeloid progenitors |
title_full | CD62L expression level determines the cell fate of myeloid progenitors |
title_fullStr | CD62L expression level determines the cell fate of myeloid progenitors |
title_full_unstemmed | CD62L expression level determines the cell fate of myeloid progenitors |
title_short | CD62L expression level determines the cell fate of myeloid progenitors |
title_sort | cd62l expression level determines the cell fate of myeloid progenitors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8693656/ https://www.ncbi.nlm.nih.gov/pubmed/34798065 http://dx.doi.org/10.1016/j.stemcr.2021.10.012 |
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