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CD62L expression level determines the cell fate of myeloid progenitors

Hematopoietic cells differentiate through several progenitors in a hierarchical manner, and recent single-cell analyses have revealed substantial heterogeneity within each progenitor. Although common myeloid progenitors (CMPs) are defined as a multipotent cell population that can differentiate into...

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Detalles Bibliográficos
Autores principales: Ito, Yusuke, Nakahara, Fumio, Kagoya, Yuki, Kurokawa, Mineo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8693656/
https://www.ncbi.nlm.nih.gov/pubmed/34798065
http://dx.doi.org/10.1016/j.stemcr.2021.10.012
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author Ito, Yusuke
Nakahara, Fumio
Kagoya, Yuki
Kurokawa, Mineo
author_facet Ito, Yusuke
Nakahara, Fumio
Kagoya, Yuki
Kurokawa, Mineo
author_sort Ito, Yusuke
collection PubMed
description Hematopoietic cells differentiate through several progenitors in a hierarchical manner, and recent single-cell analyses have revealed substantial heterogeneity within each progenitor. Although common myeloid progenitors (CMPs) are defined as a multipotent cell population that can differentiate into granulocyte-monocyte progenitors (GMPs) and megakaryocyte-erythrocyte progenitors (MEPs), and GMPs generate neutrophils and monocytes, these myeloid progenitors must contain some lineage-committed progenitors. Through gene expression analysis at single-cell levels, we identified CD62L as a marker to reveal the heterogeneity. We confirmed that CD62L-negative CMPs represent “bona fide” CMPs, whereas CD62L-high CMPs are mostly restricted to GMP potentials both in mice and humans. In addition, we identified CD62L-negative GMPs as the most immature subsets in GMPs and Ly6C(+)CD62L-intermediate and Ly6C(+)CD62L-high GMPs are skewed to neutrophil and monocyte differentiation in mice, respectively. Our findings contribute to more profound understanding about the mechanism of myeloid differentiation.
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spelling pubmed-86936562022-01-04 CD62L expression level determines the cell fate of myeloid progenitors Ito, Yusuke Nakahara, Fumio Kagoya, Yuki Kurokawa, Mineo Stem Cell Reports Article Hematopoietic cells differentiate through several progenitors in a hierarchical manner, and recent single-cell analyses have revealed substantial heterogeneity within each progenitor. Although common myeloid progenitors (CMPs) are defined as a multipotent cell population that can differentiate into granulocyte-monocyte progenitors (GMPs) and megakaryocyte-erythrocyte progenitors (MEPs), and GMPs generate neutrophils and monocytes, these myeloid progenitors must contain some lineage-committed progenitors. Through gene expression analysis at single-cell levels, we identified CD62L as a marker to reveal the heterogeneity. We confirmed that CD62L-negative CMPs represent “bona fide” CMPs, whereas CD62L-high CMPs are mostly restricted to GMP potentials both in mice and humans. In addition, we identified CD62L-negative GMPs as the most immature subsets in GMPs and Ly6C(+)CD62L-intermediate and Ly6C(+)CD62L-high GMPs are skewed to neutrophil and monocyte differentiation in mice, respectively. Our findings contribute to more profound understanding about the mechanism of myeloid differentiation. Elsevier 2021-11-18 /pmc/articles/PMC8693656/ /pubmed/34798065 http://dx.doi.org/10.1016/j.stemcr.2021.10.012 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ito, Yusuke
Nakahara, Fumio
Kagoya, Yuki
Kurokawa, Mineo
CD62L expression level determines the cell fate of myeloid progenitors
title CD62L expression level determines the cell fate of myeloid progenitors
title_full CD62L expression level determines the cell fate of myeloid progenitors
title_fullStr CD62L expression level determines the cell fate of myeloid progenitors
title_full_unstemmed CD62L expression level determines the cell fate of myeloid progenitors
title_short CD62L expression level determines the cell fate of myeloid progenitors
title_sort cd62l expression level determines the cell fate of myeloid progenitors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8693656/
https://www.ncbi.nlm.nih.gov/pubmed/34798065
http://dx.doi.org/10.1016/j.stemcr.2021.10.012
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