Identification of aldehyde oxidase 3 as a binding protein for squid ink polysaccharides using magnetic nanoparticles

To explore the interactive molecules of squid ink polysaccharides (SIP) for further understanding the action mechanisms of SIP bio-function, this study prepared SIP binding proteins from mouse liver using superparamagnetic nanometer beads. Michaelis–Menten constant (K(m)) was detected from a Linewea...

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Detalles Bibliográficos
Autores principales: Lin, Zhen, Tan, Xiaohui, Li, Fangping, Zhang, Yu, Luo, Ping, Lin, Xuan, Liu, Huazhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8694236/
https://www.ncbi.nlm.nih.gov/pubmed/35424304
http://dx.doi.org/10.1039/d0ra09222c
Descripción
Sumario:To explore the interactive molecules of squid ink polysaccharides (SIP) for further understanding the action mechanisms of SIP bio-function, this study prepared SIP binding proteins from mouse liver using superparamagnetic nanometer beads. Michaelis–Menten constant (K(m)) was detected from a Lineweaver–Burk double reciprocal plot to assess effect of SIP on activity of aldehyde oxidase (AOX). Results showed that three proteins, AOX-3, regucalcin (RGN) and α1-antitrypsin (A1AT3) were separated from mouse liver by magnetic nanoparticles conjugated with SIP. Contents of AOX-3 were much more than RGN and A1AT3. SIP (0.5 mg mL(−1)) reduced K(m) value of aldehyde oxidase of mouse liver from 91.79 μmol L(−1) to 43.70 μmol L(−1).

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