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Self-assembly of bovine serum albumin (BSA)–dextran bio-nanoconjugate: structural, antioxidant and in vitro wound healing studies

Glycation of proteins is often considered as a method to improve their functional properties for promising applications in wound healing. Furthermore, a marked increase in percentage of radical scavenging activity of the conjugates makes it an effective antioxidant synthetic strategy. A simple conju...

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Autores principales: Rohiwal, Sonali S., Ellederova, Z., Tiwari, Arpita P., Alqarni, Mohammed, Elazab, Sara T., El-Saber Batiha, Gaber, Pawar, Shivaji H., Thorat, Nanasaheb D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8694369/
https://www.ncbi.nlm.nih.gov/pubmed/35424397
http://dx.doi.org/10.1039/d0ra09301g
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author Rohiwal, Sonali S.
Ellederova, Z.
Tiwari, Arpita P.
Alqarni, Mohammed
Elazab, Sara T.
El-Saber Batiha, Gaber
Pawar, Shivaji H.
Thorat, Nanasaheb D.
author_facet Rohiwal, Sonali S.
Ellederova, Z.
Tiwari, Arpita P.
Alqarni, Mohammed
Elazab, Sara T.
El-Saber Batiha, Gaber
Pawar, Shivaji H.
Thorat, Nanasaheb D.
author_sort Rohiwal, Sonali S.
collection PubMed
description Glycation of proteins is often considered as a method to improve their functional properties for promising applications in wound healing. Furthermore, a marked increase in percentage of radical scavenging activity of the conjugates makes it an effective antioxidant synthetic strategy. A simple conjugation process was employed to develop bovine serum albumin–dextran conjugates (BSA–dextran) using Maillard reaction. Higher electrophoretic mobility and surface charge in the prepared conjugates was observed in native PAGE electrophoresis and zeta potential. Moreover, the fluorescence, FTIR and Raman analysis of the BSA–dextran conjugates shows significant shift in the fluorescence and wavelength as a consequence of conjugate formation. In vitro wound healing assay showed increased cell proliferation and migration effect. These finding suggests that BSA–dextran conjugate could open up a new practical way for exploration in the area of wound healing.
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spelling pubmed-86943692022-04-13 Self-assembly of bovine serum albumin (BSA)–dextran bio-nanoconjugate: structural, antioxidant and in vitro wound healing studies Rohiwal, Sonali S. Ellederova, Z. Tiwari, Arpita P. Alqarni, Mohammed Elazab, Sara T. El-Saber Batiha, Gaber Pawar, Shivaji H. Thorat, Nanasaheb D. RSC Adv Chemistry Glycation of proteins is often considered as a method to improve their functional properties for promising applications in wound healing. Furthermore, a marked increase in percentage of radical scavenging activity of the conjugates makes it an effective antioxidant synthetic strategy. A simple conjugation process was employed to develop bovine serum albumin–dextran conjugates (BSA–dextran) using Maillard reaction. Higher electrophoretic mobility and surface charge in the prepared conjugates was observed in native PAGE electrophoresis and zeta potential. Moreover, the fluorescence, FTIR and Raman analysis of the BSA–dextran conjugates shows significant shift in the fluorescence and wavelength as a consequence of conjugate formation. In vitro wound healing assay showed increased cell proliferation and migration effect. These finding suggests that BSA–dextran conjugate could open up a new practical way for exploration in the area of wound healing. The Royal Society of Chemistry 2021-01-21 /pmc/articles/PMC8694369/ /pubmed/35424397 http://dx.doi.org/10.1039/d0ra09301g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Rohiwal, Sonali S.
Ellederova, Z.
Tiwari, Arpita P.
Alqarni, Mohammed
Elazab, Sara T.
El-Saber Batiha, Gaber
Pawar, Shivaji H.
Thorat, Nanasaheb D.
Self-assembly of bovine serum albumin (BSA)–dextran bio-nanoconjugate: structural, antioxidant and in vitro wound healing studies
title Self-assembly of bovine serum albumin (BSA)–dextran bio-nanoconjugate: structural, antioxidant and in vitro wound healing studies
title_full Self-assembly of bovine serum albumin (BSA)–dextran bio-nanoconjugate: structural, antioxidant and in vitro wound healing studies
title_fullStr Self-assembly of bovine serum albumin (BSA)–dextran bio-nanoconjugate: structural, antioxidant and in vitro wound healing studies
title_full_unstemmed Self-assembly of bovine serum albumin (BSA)–dextran bio-nanoconjugate: structural, antioxidant and in vitro wound healing studies
title_short Self-assembly of bovine serum albumin (BSA)–dextran bio-nanoconjugate: structural, antioxidant and in vitro wound healing studies
title_sort self-assembly of bovine serum albumin (bsa)–dextran bio-nanoconjugate: structural, antioxidant and in vitro wound healing studies
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8694369/
https://www.ncbi.nlm.nih.gov/pubmed/35424397
http://dx.doi.org/10.1039/d0ra09301g
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