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Immunoassay-aptasensor for the determination of tumor-derived exosomes based on the combination of magnetic nanoparticles and hybridization chain reaction

The detection of tumor-related exosomes is of great significance. In this work, a fluorescence aptasensor was designed for the determination of tumor-related exosomes based on the capture of magnetic nanoparticles (MNPs) and specific recognition of an aptamer. MNPs were used as substrates to capture...

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Autores principales: Zhang, Hua, Zhou, Yajuan, Luo, Dan, Liu, Jingjian, Yang, E., Yang, Guangyi, Feng, Guangjun, Chen, Qinhua, Wu, Lun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8694620/
https://www.ncbi.nlm.nih.gov/pubmed/35424452
http://dx.doi.org/10.1039/d0ra10159a
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author Zhang, Hua
Zhou, Yajuan
Luo, Dan
Liu, Jingjian
Yang, E.
Yang, Guangyi
Feng, Guangjun
Chen, Qinhua
Wu, Lun
author_facet Zhang, Hua
Zhou, Yajuan
Luo, Dan
Liu, Jingjian
Yang, E.
Yang, Guangyi
Feng, Guangjun
Chen, Qinhua
Wu, Lun
author_sort Zhang, Hua
collection PubMed
description The detection of tumor-related exosomes is of great significance. In this work, a fluorescence aptasensor was designed for the determination of tumor-related exosomes based on the capture of magnetic nanoparticles (MNPs) and specific recognition of an aptamer. MNPs were used as substrates to capture the exosomes by modifying the CD63 antibody on the MNP surface. Probe 1 consists of PDL-1 aptamer sequence and a section of other sequences. PDL-1 expression was observed on the surface of exosomes; the aptamer of PDL-1 could combine with PDL-1 with high affinity. Thus, the immunoassay-type compounds of “MNPs–exosomes–probe 1” were formed. The other section of probe 1 triggered the HCR with probe 2 and probe 3 and formed the super-long dsDNA. The addition of GelRed resulted in the generation of an amplified fluorescence signal. The proposed design demonstrated a good linearity with the exosome concentration ranging from 300 to 10(7) particles per mL and with a low detection limit of 100 particles per mL. This aptasensor also exhibited high specificity for tumor-related exosomes, and was successfully applied in biological samples.
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spelling pubmed-86946202022-04-13 Immunoassay-aptasensor for the determination of tumor-derived exosomes based on the combination of magnetic nanoparticles and hybridization chain reaction Zhang, Hua Zhou, Yajuan Luo, Dan Liu, Jingjian Yang, E. Yang, Guangyi Feng, Guangjun Chen, Qinhua Wu, Lun RSC Adv Chemistry The detection of tumor-related exosomes is of great significance. In this work, a fluorescence aptasensor was designed for the determination of tumor-related exosomes based on the capture of magnetic nanoparticles (MNPs) and specific recognition of an aptamer. MNPs were used as substrates to capture the exosomes by modifying the CD63 antibody on the MNP surface. Probe 1 consists of PDL-1 aptamer sequence and a section of other sequences. PDL-1 expression was observed on the surface of exosomes; the aptamer of PDL-1 could combine with PDL-1 with high affinity. Thus, the immunoassay-type compounds of “MNPs–exosomes–probe 1” were formed. The other section of probe 1 triggered the HCR with probe 2 and probe 3 and formed the super-long dsDNA. The addition of GelRed resulted in the generation of an amplified fluorescence signal. The proposed design demonstrated a good linearity with the exosome concentration ranging from 300 to 10(7) particles per mL and with a low detection limit of 100 particles per mL. This aptasensor also exhibited high specificity for tumor-related exosomes, and was successfully applied in biological samples. The Royal Society of Chemistry 2021-01-27 /pmc/articles/PMC8694620/ /pubmed/35424452 http://dx.doi.org/10.1039/d0ra10159a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Zhang, Hua
Zhou, Yajuan
Luo, Dan
Liu, Jingjian
Yang, E.
Yang, Guangyi
Feng, Guangjun
Chen, Qinhua
Wu, Lun
Immunoassay-aptasensor for the determination of tumor-derived exosomes based on the combination of magnetic nanoparticles and hybridization chain reaction
title Immunoassay-aptasensor for the determination of tumor-derived exosomes based on the combination of magnetic nanoparticles and hybridization chain reaction
title_full Immunoassay-aptasensor for the determination of tumor-derived exosomes based on the combination of magnetic nanoparticles and hybridization chain reaction
title_fullStr Immunoassay-aptasensor for the determination of tumor-derived exosomes based on the combination of magnetic nanoparticles and hybridization chain reaction
title_full_unstemmed Immunoassay-aptasensor for the determination of tumor-derived exosomes based on the combination of magnetic nanoparticles and hybridization chain reaction
title_short Immunoassay-aptasensor for the determination of tumor-derived exosomes based on the combination of magnetic nanoparticles and hybridization chain reaction
title_sort immunoassay-aptasensor for the determination of tumor-derived exosomes based on the combination of magnetic nanoparticles and hybridization chain reaction
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8694620/
https://www.ncbi.nlm.nih.gov/pubmed/35424452
http://dx.doi.org/10.1039/d0ra10159a
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