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Effects of morphology and pore size of mesoporous silicas on the efficiency of an immobilized enzyme

An investigation is performed into the efficiency of the Streptomyces griseus HUT 6037 enzyme immobilized in three different mesoporous silicas, namely mesoporous silica film, mesocellular foam, and rod-like SBA-15. It is shown that for all three supports, the pH value changes the surface charge and...

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Autores principales: Kuo, Ping-Chung, Lin, Zhi-Xun, Wu, Tzi-Yi, Hsu, Chun-Han, Lin, Hong-Ping, Wu, Tian-Shung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8695390/
https://www.ncbi.nlm.nih.gov/pubmed/35423525
http://dx.doi.org/10.1039/d1ra01358k
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author Kuo, Ping-Chung
Lin, Zhi-Xun
Wu, Tzi-Yi
Hsu, Chun-Han
Lin, Hong-Ping
Wu, Tian-Shung
author_facet Kuo, Ping-Chung
Lin, Zhi-Xun
Wu, Tzi-Yi
Hsu, Chun-Han
Lin, Hong-Ping
Wu, Tian-Shung
author_sort Kuo, Ping-Chung
collection PubMed
description An investigation is performed into the efficiency of the Streptomyces griseus HUT 6037 enzyme immobilized in three different mesoporous silicas, namely mesoporous silica film, mesocellular foam, and rod-like SBA-15. It is shown that for all three supports, the pH value changes the surface charge and charge density and hence determines the maximum loading capacity of the enzyme. The products of the enzyme hydrolytic reaction are analyzed by (1)H-NMR. The results show that among the three silica supports, the mesoporous silica film (with a channel length in the range of 60–100 nm) maximizes the accessibility of the immobilized enzyme. The loading capacity of the enzyme is up to 95% at pH 7 and the activity of the immobilized enzyme is maintained for more than 15 days when using a silica film support. The order of the activity of the enzyme immobilized in different mesoporous silica supports is: mesoporous silica film > mesocellular foam > rod-like SBA-15. Furthermore, the immobilized enzyme can be easily separated from the reaction solution via simple filtration or centrifugation methods and re-used for hydrolytic reaction as required.
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spelling pubmed-86953902022-04-13 Effects of morphology and pore size of mesoporous silicas on the efficiency of an immobilized enzyme Kuo, Ping-Chung Lin, Zhi-Xun Wu, Tzi-Yi Hsu, Chun-Han Lin, Hong-Ping Wu, Tian-Shung RSC Adv Chemistry An investigation is performed into the efficiency of the Streptomyces griseus HUT 6037 enzyme immobilized in three different mesoporous silicas, namely mesoporous silica film, mesocellular foam, and rod-like SBA-15. It is shown that for all three supports, the pH value changes the surface charge and charge density and hence determines the maximum loading capacity of the enzyme. The products of the enzyme hydrolytic reaction are analyzed by (1)H-NMR. The results show that among the three silica supports, the mesoporous silica film (with a channel length in the range of 60–100 nm) maximizes the accessibility of the immobilized enzyme. The loading capacity of the enzyme is up to 95% at pH 7 and the activity of the immobilized enzyme is maintained for more than 15 days when using a silica film support. The order of the activity of the enzyme immobilized in different mesoporous silica supports is: mesoporous silica film > mesocellular foam > rod-like SBA-15. Furthermore, the immobilized enzyme can be easily separated from the reaction solution via simple filtration or centrifugation methods and re-used for hydrolytic reaction as required. The Royal Society of Chemistry 2021-03-08 /pmc/articles/PMC8695390/ /pubmed/35423525 http://dx.doi.org/10.1039/d1ra01358k Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Kuo, Ping-Chung
Lin, Zhi-Xun
Wu, Tzi-Yi
Hsu, Chun-Han
Lin, Hong-Ping
Wu, Tian-Shung
Effects of morphology and pore size of mesoporous silicas on the efficiency of an immobilized enzyme
title Effects of morphology and pore size of mesoporous silicas on the efficiency of an immobilized enzyme
title_full Effects of morphology and pore size of mesoporous silicas on the efficiency of an immobilized enzyme
title_fullStr Effects of morphology and pore size of mesoporous silicas on the efficiency of an immobilized enzyme
title_full_unstemmed Effects of morphology and pore size of mesoporous silicas on the efficiency of an immobilized enzyme
title_short Effects of morphology and pore size of mesoporous silicas on the efficiency of an immobilized enzyme
title_sort effects of morphology and pore size of mesoporous silicas on the efficiency of an immobilized enzyme
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8695390/
https://www.ncbi.nlm.nih.gov/pubmed/35423525
http://dx.doi.org/10.1039/d1ra01358k
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