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miR-616-5p Promotes Invasion and Migration of Bladder Cancer via Downregulating NR2C2 Expression
BACKGROUND: MicroRNAs, small non-coding RNA molecules with about 22 nucleotides in length, play a significant role in the development of bladder cancer. Previous studies found that miR-616-5p could promote the progress of cancers. However, its role in bladder cancer remains unclear. In the study, we...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8695431/ https://www.ncbi.nlm.nih.gov/pubmed/34956884 http://dx.doi.org/10.3389/fonc.2021.762946 |
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author | Ren, Wenbiao Hu, Jiao Li, Huihuang Chen, Jinbo Ding, Jian Zu, Xiongbing Fan, Benyi |
author_facet | Ren, Wenbiao Hu, Jiao Li, Huihuang Chen, Jinbo Ding, Jian Zu, Xiongbing Fan, Benyi |
author_sort | Ren, Wenbiao |
collection | PubMed |
description | BACKGROUND: MicroRNAs, small non-coding RNA molecules with about 22 nucleotides in length, play a significant role in the development of bladder cancer. Previous studies found that miR-616-5p could promote the progress of cancers. However, its role in bladder cancer remains unclear. In the study, we aimed to demonstrate how miR-616-5p impacts the invasion and migration of bladder cancer and its potential downstream targets. METHODS: Firstly, qRT-PCR was used to detect the expression of miR-616-5p in normal bladder uroepithelial cell lines and bladder cancer cell lines. Then, chamber–transwell invasion and wound healing migration assays were used to detect the roles of miR-616-5p and NR2C2 in invasion and migration. Subsequently, Western blot was used to evaluate the regulation effects of miR-616-5p and NR2C2. Finally, luciferase assays were performed to manifest the mechanism of miR-616-5p and NR2C2 regulation. RESULTS: We found that miR-616-5p was upregulated in bladder cancer, and it could promote the invasion and migration of bladder cancer in vitro. Moreover, we demonstrated that NR2C2 was a downstream target of miR-616-5p. miR-616-5p could inhibit the expression of NR2C2 by binding to the 3′UTR of NR2C2 mRNA. Importantly, patients with a high expression of NR2C2 showed better prognoses in bladder cancer. CONCLUSIONS: This study identifies that miR-616-5p can promote bladder cancer progression via altering the expression of NR2C2. Therefore, identifying miR-616-5p expression levels might be a useful strategy for developing potential therapeutic targets in bladder cancer. |
format | Online Article Text |
id | pubmed-8695431 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-86954312021-12-24 miR-616-5p Promotes Invasion and Migration of Bladder Cancer via Downregulating NR2C2 Expression Ren, Wenbiao Hu, Jiao Li, Huihuang Chen, Jinbo Ding, Jian Zu, Xiongbing Fan, Benyi Front Oncol Oncology BACKGROUND: MicroRNAs, small non-coding RNA molecules with about 22 nucleotides in length, play a significant role in the development of bladder cancer. Previous studies found that miR-616-5p could promote the progress of cancers. However, its role in bladder cancer remains unclear. In the study, we aimed to demonstrate how miR-616-5p impacts the invasion and migration of bladder cancer and its potential downstream targets. METHODS: Firstly, qRT-PCR was used to detect the expression of miR-616-5p in normal bladder uroepithelial cell lines and bladder cancer cell lines. Then, chamber–transwell invasion and wound healing migration assays were used to detect the roles of miR-616-5p and NR2C2 in invasion and migration. Subsequently, Western blot was used to evaluate the regulation effects of miR-616-5p and NR2C2. Finally, luciferase assays were performed to manifest the mechanism of miR-616-5p and NR2C2 regulation. RESULTS: We found that miR-616-5p was upregulated in bladder cancer, and it could promote the invasion and migration of bladder cancer in vitro. Moreover, we demonstrated that NR2C2 was a downstream target of miR-616-5p. miR-616-5p could inhibit the expression of NR2C2 by binding to the 3′UTR of NR2C2 mRNA. Importantly, patients with a high expression of NR2C2 showed better prognoses in bladder cancer. CONCLUSIONS: This study identifies that miR-616-5p can promote bladder cancer progression via altering the expression of NR2C2. Therefore, identifying miR-616-5p expression levels might be a useful strategy for developing potential therapeutic targets in bladder cancer. Frontiers Media S.A. 2021-12-09 /pmc/articles/PMC8695431/ /pubmed/34956884 http://dx.doi.org/10.3389/fonc.2021.762946 Text en Copyright © 2021 Ren, Hu, Li, Chen, Ding, Zu and Fan https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Oncology Ren, Wenbiao Hu, Jiao Li, Huihuang Chen, Jinbo Ding, Jian Zu, Xiongbing Fan, Benyi miR-616-5p Promotes Invasion and Migration of Bladder Cancer via Downregulating NR2C2 Expression |
title | miR-616-5p Promotes Invasion and Migration of Bladder Cancer via Downregulating NR2C2 Expression |
title_full | miR-616-5p Promotes Invasion and Migration of Bladder Cancer via Downregulating NR2C2 Expression |
title_fullStr | miR-616-5p Promotes Invasion and Migration of Bladder Cancer via Downregulating NR2C2 Expression |
title_full_unstemmed | miR-616-5p Promotes Invasion and Migration of Bladder Cancer via Downregulating NR2C2 Expression |
title_short | miR-616-5p Promotes Invasion and Migration of Bladder Cancer via Downregulating NR2C2 Expression |
title_sort | mir-616-5p promotes invasion and migration of bladder cancer via downregulating nr2c2 expression |
topic | Oncology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8695431/ https://www.ncbi.nlm.nih.gov/pubmed/34956884 http://dx.doi.org/10.3389/fonc.2021.762946 |
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