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VvMYB15 and VvWRKY40 Positively Co-regulated Anthocyanin Biosynthesis in Grape Berries in Response to Root Restriction

In most grapevine planting regions, especially in south of China, plenty of rainfall and high water level underground are the characteristic of the area, a series of problem during fruit ripening easily caused poor color quality. Thereby affecting fruit quality, yield and economic benefits. The accu...

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Autores principales: Li, Dongmei, Wang, Zhenping, Sun, Sijie, Xiao, Kun, Cao, Minghao, Li, Xiangyi, Ma, Chao, Zhang, Caixi, Wang, Lei, Lian, Hongli, Wang, Shiping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8695491/
https://www.ncbi.nlm.nih.gov/pubmed/34956287
http://dx.doi.org/10.3389/fpls.2021.789002
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author Li, Dongmei
Wang, Zhenping
Sun, Sijie
Xiao, Kun
Cao, Minghao
Li, Xiangyi
Ma, Chao
Zhang, Caixi
Wang, Lei
Lian, Hongli
Wang, Shiping
author_facet Li, Dongmei
Wang, Zhenping
Sun, Sijie
Xiao, Kun
Cao, Minghao
Li, Xiangyi
Ma, Chao
Zhang, Caixi
Wang, Lei
Lian, Hongli
Wang, Shiping
author_sort Li, Dongmei
collection PubMed
description In most grapevine planting regions, especially in south of China, plenty of rainfall and high water level underground are the characteristic of the area, a series of problem during fruit ripening easily caused poor color quality. Thereby affecting fruit quality, yield and economic benefits. The accumulation of anthocyanin is regulated by transcriptional regulatory factor and a series of cultivation measures, root restriction can make plants in the environment of stress and stress relief, root restriction induced the higher expression of VvMYB15 and VvWRKY40, and consistent with anthocyanin accumulation. Whether and how root restriction-inducible VvMYB15 and VvWRKY40 transcription factor regulate anthocyanin synthesis in grape berry is still unclear. In this study, we identified that the transient overexpression of VvMYB15 and VvWRKY40 alone or both in strawberry fruits and grape berries can promote anthocyanin accumulation and increase the expression level of anthocyanin biosynthetic genes, indicating VvMYB15 and VvWRKY40 play a positive regulator of anthocyanin biosynthesis. Furthermore, we confirmed that both VvMYB15 and VvWRKY40 specifically bind to the promoter region of VvF3′5′H and VvUFGT, and the expression of VvF3′5′H and VvUFGT is further activated through the heterodimer formation between VvMYB15 and VvWRKY40. Finally, we confirmed that VvMYB15 promoted anthocyanin accumulation by interacting with VvWRKY40 in grape berries, our findings provide insights into a mechanism involving the synergistic regulation of root restriction-dependent coloration and biosynthesis via a VvMYB15 and VvWRKY40 alone or both in grape berries.
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spelling pubmed-86954912021-12-24 VvMYB15 and VvWRKY40 Positively Co-regulated Anthocyanin Biosynthesis in Grape Berries in Response to Root Restriction Li, Dongmei Wang, Zhenping Sun, Sijie Xiao, Kun Cao, Minghao Li, Xiangyi Ma, Chao Zhang, Caixi Wang, Lei Lian, Hongli Wang, Shiping Front Plant Sci Plant Science In most grapevine planting regions, especially in south of China, plenty of rainfall and high water level underground are the characteristic of the area, a series of problem during fruit ripening easily caused poor color quality. Thereby affecting fruit quality, yield and economic benefits. The accumulation of anthocyanin is regulated by transcriptional regulatory factor and a series of cultivation measures, root restriction can make plants in the environment of stress and stress relief, root restriction induced the higher expression of VvMYB15 and VvWRKY40, and consistent with anthocyanin accumulation. Whether and how root restriction-inducible VvMYB15 and VvWRKY40 transcription factor regulate anthocyanin synthesis in grape berry is still unclear. In this study, we identified that the transient overexpression of VvMYB15 and VvWRKY40 alone or both in strawberry fruits and grape berries can promote anthocyanin accumulation and increase the expression level of anthocyanin biosynthetic genes, indicating VvMYB15 and VvWRKY40 play a positive regulator of anthocyanin biosynthesis. Furthermore, we confirmed that both VvMYB15 and VvWRKY40 specifically bind to the promoter region of VvF3′5′H and VvUFGT, and the expression of VvF3′5′H and VvUFGT is further activated through the heterodimer formation between VvMYB15 and VvWRKY40. Finally, we confirmed that VvMYB15 promoted anthocyanin accumulation by interacting with VvWRKY40 in grape berries, our findings provide insights into a mechanism involving the synergistic regulation of root restriction-dependent coloration and biosynthesis via a VvMYB15 and VvWRKY40 alone or both in grape berries. Frontiers Media S.A. 2021-12-09 /pmc/articles/PMC8695491/ /pubmed/34956287 http://dx.doi.org/10.3389/fpls.2021.789002 Text en Copyright © 2021 Li, Wang, Sun, Xiao, Cao, Li, Ma, Zhang, Wang, Lian and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Li, Dongmei
Wang, Zhenping
Sun, Sijie
Xiao, Kun
Cao, Minghao
Li, Xiangyi
Ma, Chao
Zhang, Caixi
Wang, Lei
Lian, Hongli
Wang, Shiping
VvMYB15 and VvWRKY40 Positively Co-regulated Anthocyanin Biosynthesis in Grape Berries in Response to Root Restriction
title VvMYB15 and VvWRKY40 Positively Co-regulated Anthocyanin Biosynthesis in Grape Berries in Response to Root Restriction
title_full VvMYB15 and VvWRKY40 Positively Co-regulated Anthocyanin Biosynthesis in Grape Berries in Response to Root Restriction
title_fullStr VvMYB15 and VvWRKY40 Positively Co-regulated Anthocyanin Biosynthesis in Grape Berries in Response to Root Restriction
title_full_unstemmed VvMYB15 and VvWRKY40 Positively Co-regulated Anthocyanin Biosynthesis in Grape Berries in Response to Root Restriction
title_short VvMYB15 and VvWRKY40 Positively Co-regulated Anthocyanin Biosynthesis in Grape Berries in Response to Root Restriction
title_sort vvmyb15 and vvwrky40 positively co-regulated anthocyanin biosynthesis in grape berries in response to root restriction
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8695491/
https://www.ncbi.nlm.nih.gov/pubmed/34956287
http://dx.doi.org/10.3389/fpls.2021.789002
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