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A Novel Chloroplast Protein RNA Processing 8 Is Required for the Expression of Chloroplast Genes and Chloroplast Development in Arabidopsis thaliana

Chloroplast development involves the coordinated expression of both plastids- and nuclear-encoded genes in higher plants. However, the underlying mechanism still remains largely unknown. In this study, we isolated and characterized an Arabidopsis mutant with an albino lethality phenotype named RNA p...

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Autores principales: Kong, Mengmeng, Wu, Yaozong, Wang, Ziyuan, Qu, Wantong, Lan, Yixin, Chen, Xin, Liu, Yanyun, Shahnaz, Perveen, Yang, Zhongnan, Yu, Qingbo, Mi, Hualing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8695849/
https://www.ncbi.nlm.nih.gov/pubmed/34956248
http://dx.doi.org/10.3389/fpls.2021.700975
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author Kong, Mengmeng
Wu, Yaozong
Wang, Ziyuan
Qu, Wantong
Lan, Yixin
Chen, Xin
Liu, Yanyun
Shahnaz, Perveen
Yang, Zhongnan
Yu, Qingbo
Mi, Hualing
author_facet Kong, Mengmeng
Wu, Yaozong
Wang, Ziyuan
Qu, Wantong
Lan, Yixin
Chen, Xin
Liu, Yanyun
Shahnaz, Perveen
Yang, Zhongnan
Yu, Qingbo
Mi, Hualing
author_sort Kong, Mengmeng
collection PubMed
description Chloroplast development involves the coordinated expression of both plastids- and nuclear-encoded genes in higher plants. However, the underlying mechanism still remains largely unknown. In this study, we isolated and characterized an Arabidopsis mutant with an albino lethality phenotype named RNA processing 8 (rp8). Genetic complementation analysis demonstrated that the gene AT4G37920 (RP8) was responsible for the mutated phenotype. The RP8 gene was strongly expressed in photosynthetic tissues at both transcription and translation protein levels. The RP8 protein is localized in the chloroplast and associated with the thylakoid. Disruption of the RP8 gene led to a defect in the accumulation of the rpoA mature transcript, which reduced the level of the RpoA protein, and affected the transcription of PEP-dependent genes. The abundance of the chloroplast rRNA, including 23S, 16S, 4.5S, and 5S rRNA, were reduced in the rp8 mutant, respectively, and the amounts of chloroplast ribosome proteins, such as, PRPS1(uS1c), PRPS5(uS5c), PRPL2 (uL2c), and PRPL4 (uL4c), were substantially decreased in the rp8 mutant, which indicated that knockout of RP8 seriously affected chloroplast translational machinery. Accordingly, the accumulation of photosynthetic proteins was seriously reduced. Taken together, these results indicate that the RP8 protein plays an important regulatory role in the rpoA transcript processing, which is required for the expression of chloroplast genes and chloroplast development in Arabidopsis.
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spelling pubmed-86958492021-12-24 A Novel Chloroplast Protein RNA Processing 8 Is Required for the Expression of Chloroplast Genes and Chloroplast Development in Arabidopsis thaliana Kong, Mengmeng Wu, Yaozong Wang, Ziyuan Qu, Wantong Lan, Yixin Chen, Xin Liu, Yanyun Shahnaz, Perveen Yang, Zhongnan Yu, Qingbo Mi, Hualing Front Plant Sci Plant Science Chloroplast development involves the coordinated expression of both plastids- and nuclear-encoded genes in higher plants. However, the underlying mechanism still remains largely unknown. In this study, we isolated and characterized an Arabidopsis mutant with an albino lethality phenotype named RNA processing 8 (rp8). Genetic complementation analysis demonstrated that the gene AT4G37920 (RP8) was responsible for the mutated phenotype. The RP8 gene was strongly expressed in photosynthetic tissues at both transcription and translation protein levels. The RP8 protein is localized in the chloroplast and associated with the thylakoid. Disruption of the RP8 gene led to a defect in the accumulation of the rpoA mature transcript, which reduced the level of the RpoA protein, and affected the transcription of PEP-dependent genes. The abundance of the chloroplast rRNA, including 23S, 16S, 4.5S, and 5S rRNA, were reduced in the rp8 mutant, respectively, and the amounts of chloroplast ribosome proteins, such as, PRPS1(uS1c), PRPS5(uS5c), PRPL2 (uL2c), and PRPL4 (uL4c), were substantially decreased in the rp8 mutant, which indicated that knockout of RP8 seriously affected chloroplast translational machinery. Accordingly, the accumulation of photosynthetic proteins was seriously reduced. Taken together, these results indicate that the RP8 protein plays an important regulatory role in the rpoA transcript processing, which is required for the expression of chloroplast genes and chloroplast development in Arabidopsis. Frontiers Media S.A. 2021-12-09 /pmc/articles/PMC8695849/ /pubmed/34956248 http://dx.doi.org/10.3389/fpls.2021.700975 Text en Copyright © 2021 Kong, Wu, Wang, Qu, Lan, Chen, Liu, Shahnaz, Yang, Yu and Mi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Kong, Mengmeng
Wu, Yaozong
Wang, Ziyuan
Qu, Wantong
Lan, Yixin
Chen, Xin
Liu, Yanyun
Shahnaz, Perveen
Yang, Zhongnan
Yu, Qingbo
Mi, Hualing
A Novel Chloroplast Protein RNA Processing 8 Is Required for the Expression of Chloroplast Genes and Chloroplast Development in Arabidopsis thaliana
title A Novel Chloroplast Protein RNA Processing 8 Is Required for the Expression of Chloroplast Genes and Chloroplast Development in Arabidopsis thaliana
title_full A Novel Chloroplast Protein RNA Processing 8 Is Required for the Expression of Chloroplast Genes and Chloroplast Development in Arabidopsis thaliana
title_fullStr A Novel Chloroplast Protein RNA Processing 8 Is Required for the Expression of Chloroplast Genes and Chloroplast Development in Arabidopsis thaliana
title_full_unstemmed A Novel Chloroplast Protein RNA Processing 8 Is Required for the Expression of Chloroplast Genes and Chloroplast Development in Arabidopsis thaliana
title_short A Novel Chloroplast Protein RNA Processing 8 Is Required for the Expression of Chloroplast Genes and Chloroplast Development in Arabidopsis thaliana
title_sort novel chloroplast protein rna processing 8 is required for the expression of chloroplast genes and chloroplast development in arabidopsis thaliana
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8695849/
https://www.ncbi.nlm.nih.gov/pubmed/34956248
http://dx.doi.org/10.3389/fpls.2021.700975
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