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Targeted Methylation of the LncRNA NEAT1 Suppresses Malignancy of Renal Cell Carcinoma
Long-chain non-coding RNA (LncRNA) has been found to play an important role in the regulation of the occurrence and progression of renal cell carcinoma (RCC). In this study, we demonstrated that LncRNA NEAT1 expression and m6A methylation level was decreased in RCC tissues. Further, the downregulate...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8696001/ https://www.ncbi.nlm.nih.gov/pubmed/34957107 http://dx.doi.org/10.3389/fcell.2021.777349 |
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author | Chen, Jieqing Liao, Xinhui Cheng, Jianli Su, Ganglin Yuan, Fen Zhang, Zhongfu Wu, Jianting Mei, Hongbing Tan, Wanlong |
author_facet | Chen, Jieqing Liao, Xinhui Cheng, Jianli Su, Ganglin Yuan, Fen Zhang, Zhongfu Wu, Jianting Mei, Hongbing Tan, Wanlong |
author_sort | Chen, Jieqing |
collection | PubMed |
description | Long-chain non-coding RNA (LncRNA) has been found to play an important role in the regulation of the occurrence and progression of renal cell carcinoma (RCC). In this study, we demonstrated that LncRNA NEAT1 expression and m6A methylation level was decreased in RCC tissues. Further, the downregulated expression level of LncRNA NEAT1 was associated with poor prognosis for RCC patients. Then we used CRIPSR/dCas13b-METTL3 to methylate LncRNA NEAT1 in RCC cells. The results showed that the expression level of LncRNA NEAT1 was upregulated after methylated by dCas13b-METTL3 in RCC cells. And the proliferation and migration ability of RCC cells was decreased after methylated LncRNA NEAT1. Finally, we examined the effect of LncRNA NEAT1 hypermethylation on the transcriptome. We found differentially expressed genes in RCC cells were associated with “cGMP-PKG signaling pathway”, “Cell adhesion molecules” and “Pathways in cancer”. In conclusion, CRISPR/Cas13b-METTL3 targeting LncRNA NEAT1 m6A methylation activates LncRNA NEAT1 expression and provides a new target for treatment of RCC. |
format | Online Article Text |
id | pubmed-8696001 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-86960012021-12-24 Targeted Methylation of the LncRNA NEAT1 Suppresses Malignancy of Renal Cell Carcinoma Chen, Jieqing Liao, Xinhui Cheng, Jianli Su, Ganglin Yuan, Fen Zhang, Zhongfu Wu, Jianting Mei, Hongbing Tan, Wanlong Front Cell Dev Biol Cell and Developmental Biology Long-chain non-coding RNA (LncRNA) has been found to play an important role in the regulation of the occurrence and progression of renal cell carcinoma (RCC). In this study, we demonstrated that LncRNA NEAT1 expression and m6A methylation level was decreased in RCC tissues. Further, the downregulated expression level of LncRNA NEAT1 was associated with poor prognosis for RCC patients. Then we used CRIPSR/dCas13b-METTL3 to methylate LncRNA NEAT1 in RCC cells. The results showed that the expression level of LncRNA NEAT1 was upregulated after methylated by dCas13b-METTL3 in RCC cells. And the proliferation and migration ability of RCC cells was decreased after methylated LncRNA NEAT1. Finally, we examined the effect of LncRNA NEAT1 hypermethylation on the transcriptome. We found differentially expressed genes in RCC cells were associated with “cGMP-PKG signaling pathway”, “Cell adhesion molecules” and “Pathways in cancer”. In conclusion, CRISPR/Cas13b-METTL3 targeting LncRNA NEAT1 m6A methylation activates LncRNA NEAT1 expression and provides a new target for treatment of RCC. Frontiers Media S.A. 2021-12-09 /pmc/articles/PMC8696001/ /pubmed/34957107 http://dx.doi.org/10.3389/fcell.2021.777349 Text en Copyright © 2021 Chen, Liao, Cheng, Su, Yuan, Zhang, Wu, Mei and Tan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Chen, Jieqing Liao, Xinhui Cheng, Jianli Su, Ganglin Yuan, Fen Zhang, Zhongfu Wu, Jianting Mei, Hongbing Tan, Wanlong Targeted Methylation of the LncRNA NEAT1 Suppresses Malignancy of Renal Cell Carcinoma |
title | Targeted Methylation of the LncRNA NEAT1 Suppresses Malignancy of Renal Cell Carcinoma |
title_full | Targeted Methylation of the LncRNA NEAT1 Suppresses Malignancy of Renal Cell Carcinoma |
title_fullStr | Targeted Methylation of the LncRNA NEAT1 Suppresses Malignancy of Renal Cell Carcinoma |
title_full_unstemmed | Targeted Methylation of the LncRNA NEAT1 Suppresses Malignancy of Renal Cell Carcinoma |
title_short | Targeted Methylation of the LncRNA NEAT1 Suppresses Malignancy of Renal Cell Carcinoma |
title_sort | targeted methylation of the lncrna neat1 suppresses malignancy of renal cell carcinoma |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8696001/ https://www.ncbi.nlm.nih.gov/pubmed/34957107 http://dx.doi.org/10.3389/fcell.2021.777349 |
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