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Identification and Functional Verification of Cold Tolerance Genes in Spring Maize Seedlings Based on a Genome-Wide Association Study and Quantitative Trait Locus Mapping

Maize (Zea mays L.) is a tropical crop, and low temperature has become one of the main abiotic stresses for maize growth and development, affecting many maize growth processes. The main area of maize production in China, Jilin province, often suffers from varying degrees of cold damage in spring, wh...

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Autores principales: Jin, Yukun, Zhang, Zhongren, Xi, Yongjing, Yang, Zhou, Xiao, Zhifeng, Guan, Shuyan, Qu, Jing, Wang, Piwu, Zhao, Rengui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8696014/
https://www.ncbi.nlm.nih.gov/pubmed/34956272
http://dx.doi.org/10.3389/fpls.2021.776972
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author Jin, Yukun
Zhang, Zhongren
Xi, Yongjing
Yang, Zhou
Xiao, Zhifeng
Guan, Shuyan
Qu, Jing
Wang, Piwu
Zhao, Rengui
author_facet Jin, Yukun
Zhang, Zhongren
Xi, Yongjing
Yang, Zhou
Xiao, Zhifeng
Guan, Shuyan
Qu, Jing
Wang, Piwu
Zhao, Rengui
author_sort Jin, Yukun
collection PubMed
description Maize (Zea mays L.) is a tropical crop, and low temperature has become one of the main abiotic stresses for maize growth and development, affecting many maize growth processes. The main area of maize production in China, Jilin province, often suffers from varying degrees of cold damage in spring, which seriously affects the quality and yield of maize. In the face of global climate change and food security concerns, discovering cold tolerance genes, developing cold tolerance molecular markers, and creating cold-tolerant germplasm have become urgent for improving maize resilience against these conditions and obtaining an increase in overall yield. In this study, whole-genome sequencing and genotyping by sequencing were used to perform genome-wide association analysis (GWAS) and quantitative trait locus (QTL) mapping of the two populations, respectively. Overall, four single-nucleotide polymorphisms (SNPs) and 12 QTLs were found to be significantly associated with cold tolerance. Through joint analysis, an intersection of GWAS and QTL mapping was found on chromosome 3, on which the Zm00001d002729 gene was identified as a potential factor in cold tolerance. We verified the function of this target gene through overexpression, suppression of expression, and genetic transformation into maize. We found that Zm00001d002729 overexpression resulted in better cold tolerance in this crop. The identification of genes associated with cold tolerance contributes to the clarification of the underlying mechanism of this trait in maize and provides a foundation for the adaptation of maize to colder environments in the future, to ensure food security.
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spelling pubmed-86960142021-12-24 Identification and Functional Verification of Cold Tolerance Genes in Spring Maize Seedlings Based on a Genome-Wide Association Study and Quantitative Trait Locus Mapping Jin, Yukun Zhang, Zhongren Xi, Yongjing Yang, Zhou Xiao, Zhifeng Guan, Shuyan Qu, Jing Wang, Piwu Zhao, Rengui Front Plant Sci Plant Science Maize (Zea mays L.) is a tropical crop, and low temperature has become one of the main abiotic stresses for maize growth and development, affecting many maize growth processes. The main area of maize production in China, Jilin province, often suffers from varying degrees of cold damage in spring, which seriously affects the quality and yield of maize. In the face of global climate change and food security concerns, discovering cold tolerance genes, developing cold tolerance molecular markers, and creating cold-tolerant germplasm have become urgent for improving maize resilience against these conditions and obtaining an increase in overall yield. In this study, whole-genome sequencing and genotyping by sequencing were used to perform genome-wide association analysis (GWAS) and quantitative trait locus (QTL) mapping of the two populations, respectively. Overall, four single-nucleotide polymorphisms (SNPs) and 12 QTLs were found to be significantly associated with cold tolerance. Through joint analysis, an intersection of GWAS and QTL mapping was found on chromosome 3, on which the Zm00001d002729 gene was identified as a potential factor in cold tolerance. We verified the function of this target gene through overexpression, suppression of expression, and genetic transformation into maize. We found that Zm00001d002729 overexpression resulted in better cold tolerance in this crop. The identification of genes associated with cold tolerance contributes to the clarification of the underlying mechanism of this trait in maize and provides a foundation for the adaptation of maize to colder environments in the future, to ensure food security. Frontiers Media S.A. 2021-12-09 /pmc/articles/PMC8696014/ /pubmed/34956272 http://dx.doi.org/10.3389/fpls.2021.776972 Text en Copyright © 2021 Jin, Zhang, Xi, Yang, Xiao, Guan, Qu, Wang and Zhao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Jin, Yukun
Zhang, Zhongren
Xi, Yongjing
Yang, Zhou
Xiao, Zhifeng
Guan, Shuyan
Qu, Jing
Wang, Piwu
Zhao, Rengui
Identification and Functional Verification of Cold Tolerance Genes in Spring Maize Seedlings Based on a Genome-Wide Association Study and Quantitative Trait Locus Mapping
title Identification and Functional Verification of Cold Tolerance Genes in Spring Maize Seedlings Based on a Genome-Wide Association Study and Quantitative Trait Locus Mapping
title_full Identification and Functional Verification of Cold Tolerance Genes in Spring Maize Seedlings Based on a Genome-Wide Association Study and Quantitative Trait Locus Mapping
title_fullStr Identification and Functional Verification of Cold Tolerance Genes in Spring Maize Seedlings Based on a Genome-Wide Association Study and Quantitative Trait Locus Mapping
title_full_unstemmed Identification and Functional Verification of Cold Tolerance Genes in Spring Maize Seedlings Based on a Genome-Wide Association Study and Quantitative Trait Locus Mapping
title_short Identification and Functional Verification of Cold Tolerance Genes in Spring Maize Seedlings Based on a Genome-Wide Association Study and Quantitative Trait Locus Mapping
title_sort identification and functional verification of cold tolerance genes in spring maize seedlings based on a genome-wide association study and quantitative trait locus mapping
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8696014/
https://www.ncbi.nlm.nih.gov/pubmed/34956272
http://dx.doi.org/10.3389/fpls.2021.776972
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