Cargando…
Compound Kushen Injection Protects Skin From Radiation Injury via Regulating Bim
Background: Radiation-induced skin injury is a major side-effect observed in cancer patients who received radiotherapy. Thus identifying new radioprotective drugs for prevention or treatment of post-irradiation skin injury should be prompted. A large number of clinical studies have confirmed that Co...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8696473/ https://www.ncbi.nlm.nih.gov/pubmed/34955827 http://dx.doi.org/10.3389/fphar.2021.753068 |
_version_ | 1784619821198999552 |
---|---|
author | Zheng, Jianxiao Li, Gong Wang, Juanjuan Wang, Shujing Tang, Qing Sheng, Honghao Wu, Wanyin Wang, Sumei |
author_facet | Zheng, Jianxiao Li, Gong Wang, Juanjuan Wang, Shujing Tang, Qing Sheng, Honghao Wu, Wanyin Wang, Sumei |
author_sort | Zheng, Jianxiao |
collection | PubMed |
description | Background: Radiation-induced skin injury is a major side-effect observed in cancer patients who received radiotherapy. Thus identifying new radioprotective drugs for prevention or treatment of post-irradiation skin injury should be prompted. A large number of clinical studies have confirmed that Compound Kushen injection (CKI) can enhance efficacy and reduce toxicity of radiotherapy. The aim of this study is to confirm the effect of CKI in alleviating radiotherapy injury in the skin and explore the exact mechanism. Methods: 60 patients who met the inclusion/exclusion criteria were allocated to treatment group (CKI before radiotherapy) or control group (normal saline before radiotherapy) randomly. MTT assay, flow cytometry, Western Blot, and transient transfection were performed to detect the cell viability, cell apoptosis and Bim expression after treatment with CKI or/and radiotherapy. Results: CKI had the effect of alleviating skin injury in cancer patients who received radiotherapy in clinic. CKI induced cancer cell apoptosis when combined with irradiation (IR), while it reversed the induction of cell apoptosis by IR in human skin fibroblast (HSF) cells. And Bim, as a tumor suppressor, was induced in cancer cells but had no change in HSF cells when treated with CKI. Moreover, the above effect could be attenuated when Bim was silenced by siRNA. Conclusion: We conclude that CKI represents a promising radio-protective agent with a potential differential beneficial effect on both cancer cells (inducing apoptosis) and HSF cells (providing radio-protection via inhibiting IR-induced apoptosis), via regulating Bim. Our study uncovers a novel mechanism by which CKI inhibits human cancer cell while protects skin from radiotherapy, indicating CKI might be a promising radio-protective drug. Clinical Trial Registration: Chinese Clinical Trial Registry (www.chictr.org.cn), identifier ChiCTR2100049164. |
format | Online Article Text |
id | pubmed-8696473 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-86964732021-12-24 Compound Kushen Injection Protects Skin From Radiation Injury via Regulating Bim Zheng, Jianxiao Li, Gong Wang, Juanjuan Wang, Shujing Tang, Qing Sheng, Honghao Wu, Wanyin Wang, Sumei Front Pharmacol Pharmacology Background: Radiation-induced skin injury is a major side-effect observed in cancer patients who received radiotherapy. Thus identifying new radioprotective drugs for prevention or treatment of post-irradiation skin injury should be prompted. A large number of clinical studies have confirmed that Compound Kushen injection (CKI) can enhance efficacy and reduce toxicity of radiotherapy. The aim of this study is to confirm the effect of CKI in alleviating radiotherapy injury in the skin and explore the exact mechanism. Methods: 60 patients who met the inclusion/exclusion criteria were allocated to treatment group (CKI before radiotherapy) or control group (normal saline before radiotherapy) randomly. MTT assay, flow cytometry, Western Blot, and transient transfection were performed to detect the cell viability, cell apoptosis and Bim expression after treatment with CKI or/and radiotherapy. Results: CKI had the effect of alleviating skin injury in cancer patients who received radiotherapy in clinic. CKI induced cancer cell apoptosis when combined with irradiation (IR), while it reversed the induction of cell apoptosis by IR in human skin fibroblast (HSF) cells. And Bim, as a tumor suppressor, was induced in cancer cells but had no change in HSF cells when treated with CKI. Moreover, the above effect could be attenuated when Bim was silenced by siRNA. Conclusion: We conclude that CKI represents a promising radio-protective agent with a potential differential beneficial effect on both cancer cells (inducing apoptosis) and HSF cells (providing radio-protection via inhibiting IR-induced apoptosis), via regulating Bim. Our study uncovers a novel mechanism by which CKI inhibits human cancer cell while protects skin from radiotherapy, indicating CKI might be a promising radio-protective drug. Clinical Trial Registration: Chinese Clinical Trial Registry (www.chictr.org.cn), identifier ChiCTR2100049164. Frontiers Media S.A. 2021-12-09 /pmc/articles/PMC8696473/ /pubmed/34955827 http://dx.doi.org/10.3389/fphar.2021.753068 Text en Copyright © 2021 Zheng, Li, Wang, Wang, Tang, Sheng, Wu and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Zheng, Jianxiao Li, Gong Wang, Juanjuan Wang, Shujing Tang, Qing Sheng, Honghao Wu, Wanyin Wang, Sumei Compound Kushen Injection Protects Skin From Radiation Injury via Regulating Bim |
title | Compound Kushen Injection Protects Skin From Radiation Injury via Regulating Bim |
title_full | Compound Kushen Injection Protects Skin From Radiation Injury via Regulating Bim |
title_fullStr | Compound Kushen Injection Protects Skin From Radiation Injury via Regulating Bim |
title_full_unstemmed | Compound Kushen Injection Protects Skin From Radiation Injury via Regulating Bim |
title_short | Compound Kushen Injection Protects Skin From Radiation Injury via Regulating Bim |
title_sort | compound kushen injection protects skin from radiation injury via regulating bim |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8696473/ https://www.ncbi.nlm.nih.gov/pubmed/34955827 http://dx.doi.org/10.3389/fphar.2021.753068 |
work_keys_str_mv | AT zhengjianxiao compoundkusheninjectionprotectsskinfromradiationinjuryviaregulatingbim AT ligong compoundkusheninjectionprotectsskinfromradiationinjuryviaregulatingbim AT wangjuanjuan compoundkusheninjectionprotectsskinfromradiationinjuryviaregulatingbim AT wangshujing compoundkusheninjectionprotectsskinfromradiationinjuryviaregulatingbim AT tangqing compoundkusheninjectionprotectsskinfromradiationinjuryviaregulatingbim AT shenghonghao compoundkusheninjectionprotectsskinfromradiationinjuryviaregulatingbim AT wuwanyin compoundkusheninjectionprotectsskinfromradiationinjuryviaregulatingbim AT wangsumei compoundkusheninjectionprotectsskinfromradiationinjuryviaregulatingbim |