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Mitochondrial-Respiration-Improving Effects of Three Different Gardeniae Fructus Preparations and Their Components

[Image: see text] The processing method for Chinese traditional herbal medicine is “Pao Zhi” in Chinese. This study examined the efficacy of the Pao Zhi on the preparations of Gardeniae Fructus (GF) on a mitochondrial respiratory function in rats. To determine the efficacy of Pao Zhi, we investigate...

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Autores principales: Wang, Yun, Li, Puling, Zhang, Xue, Li, Lingyun, Liu, Mengjiao, Li, Xiaoqing, Dai, Yejia, Zhang, Cun, Li, Shaojing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8697009/
https://www.ncbi.nlm.nih.gov/pubmed/34963909
http://dx.doi.org/10.1021/acsomega.1c03265
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author Wang, Yun
Li, Puling
Zhang, Xue
Li, Lingyun
Liu, Mengjiao
Li, Xiaoqing
Dai, Yejia
Zhang, Cun
Li, Shaojing
author_facet Wang, Yun
Li, Puling
Zhang, Xue
Li, Lingyun
Liu, Mengjiao
Li, Xiaoqing
Dai, Yejia
Zhang, Cun
Li, Shaojing
author_sort Wang, Yun
collection PubMed
description [Image: see text] The processing method for Chinese traditional herbal medicine is “Pao Zhi” in Chinese. This study examined the efficacy of the Pao Zhi on the preparations of Gardeniae Fructus (GF) on a mitochondrial respiratory function in rats. To determine the efficacy of Pao Zhi, we investigated the effects of GF heat processing on mitochondrial respiratory function. To test the GF components, the rats were randomly divided into a geniposide-alone group, crocin-alone group, and combination groups and treated with geniposide and crocin at different ratios. The results showed that a high dose, raw GF was more effective in improving the neurological function, mitochondrial respiratory function, and activities of Na(+)-K(+)-ATPase and Ca(2+)-Mg(2+)-ATPase than the preparations that underwent heating. Moreover, mitochondrial ROS production was the lowest in the raw GF-treated group. In addition, treatments with crocin and GC3 were more effective than geniposide in improving the functional deficit in MCAO rats. In conclusion, our results suggest that raw GF is the most suitable preparation for the treatment of cerebral ischemia, and its underlying mechanisms may be associated with the improvement of mitochondrial respiratory function, increased activities of Na(+)-K(+)-ATPase and Ca(2+)-Mg(2+)-ATPase, and reduced oxidative stress in mitochondria. Our findings suggest that raw GF, especially crocin, could be an ideal therapeutic agent for ischemic stroke.
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spelling pubmed-86970092021-12-27 Mitochondrial-Respiration-Improving Effects of Three Different Gardeniae Fructus Preparations and Their Components Wang, Yun Li, Puling Zhang, Xue Li, Lingyun Liu, Mengjiao Li, Xiaoqing Dai, Yejia Zhang, Cun Li, Shaojing ACS Omega [Image: see text] The processing method for Chinese traditional herbal medicine is “Pao Zhi” in Chinese. This study examined the efficacy of the Pao Zhi on the preparations of Gardeniae Fructus (GF) on a mitochondrial respiratory function in rats. To determine the efficacy of Pao Zhi, we investigated the effects of GF heat processing on mitochondrial respiratory function. To test the GF components, the rats were randomly divided into a geniposide-alone group, crocin-alone group, and combination groups and treated with geniposide and crocin at different ratios. The results showed that a high dose, raw GF was more effective in improving the neurological function, mitochondrial respiratory function, and activities of Na(+)-K(+)-ATPase and Ca(2+)-Mg(2+)-ATPase than the preparations that underwent heating. Moreover, mitochondrial ROS production was the lowest in the raw GF-treated group. In addition, treatments with crocin and GC3 were more effective than geniposide in improving the functional deficit in MCAO rats. In conclusion, our results suggest that raw GF is the most suitable preparation for the treatment of cerebral ischemia, and its underlying mechanisms may be associated with the improvement of mitochondrial respiratory function, increased activities of Na(+)-K(+)-ATPase and Ca(2+)-Mg(2+)-ATPase, and reduced oxidative stress in mitochondria. Our findings suggest that raw GF, especially crocin, could be an ideal therapeutic agent for ischemic stroke. American Chemical Society 2021-12-07 /pmc/articles/PMC8697009/ /pubmed/34963909 http://dx.doi.org/10.1021/acsomega.1c03265 Text en © 2021 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Wang, Yun
Li, Puling
Zhang, Xue
Li, Lingyun
Liu, Mengjiao
Li, Xiaoqing
Dai, Yejia
Zhang, Cun
Li, Shaojing
Mitochondrial-Respiration-Improving Effects of Three Different Gardeniae Fructus Preparations and Their Components
title Mitochondrial-Respiration-Improving Effects of Three Different Gardeniae Fructus Preparations and Their Components
title_full Mitochondrial-Respiration-Improving Effects of Three Different Gardeniae Fructus Preparations and Their Components
title_fullStr Mitochondrial-Respiration-Improving Effects of Three Different Gardeniae Fructus Preparations and Their Components
title_full_unstemmed Mitochondrial-Respiration-Improving Effects of Three Different Gardeniae Fructus Preparations and Their Components
title_short Mitochondrial-Respiration-Improving Effects of Three Different Gardeniae Fructus Preparations and Their Components
title_sort mitochondrial-respiration-improving effects of three different gardeniae fructus preparations and their components
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8697009/
https://www.ncbi.nlm.nih.gov/pubmed/34963909
http://dx.doi.org/10.1021/acsomega.1c03265
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