Toxigenic Clostridium difficile-mediated diarrhoea in hematopoietic stem cell transplantation in-patients: rapid diagnosis and efficient treatment

Allogenic hematopoietic stem cell transplant (HSCT) recipients are susceptible to any kind of infectious agents including Clostridium difficile. We studied 86 allogenic-HSCT patients who faced diarrhoea while receiving antibiotics. DNA from stool samples were explored for the presence of C. difficil...

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Autores principales: Khodaparast, Sepideh, Mohabati Mobarez, Ashraf, Khoramabadi, Nima, Vasei, Mohammad, Saberifiroozi, Mehdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cambridge University Press 2021
Materias:
Short Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8697310/
https://www.ncbi.nlm.nih.gov/pubmed/34372951
http://dx.doi.org/10.1017/S095026882100162X
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author Khodaparast, Sepideh
Mohabati Mobarez, Ashraf
Khoramabadi, Nima
Vasei, Mohammad
Saberifiroozi, Mehdi
author_facet Khodaparast, Sepideh
Mohabati Mobarez, Ashraf
Khoramabadi, Nima
Vasei, Mohammad
Saberifiroozi, Mehdi
author_sort Khodaparast, Sepideh
collection PubMed
description Allogenic hematopoietic stem cell transplant (HSCT) recipients are susceptible to any kind of infectious agents including Clostridium difficile. We studied 86 allogenic-HSCT patients who faced diarrhoea while receiving antibiotics. DNA from stool samples were explored for the presence of C. difficile toxin genes (tcdA; tcdB) by multiplex real-time PCR. Results showed nine toxigenic C. difficile amongst which seven were positive for both toxins and two were positive for tcdB. Six of toxigenic C. difficile organisms harbouring both toxin genes were also isolated by toxigenic culture. Clostridium difficile infection was controlled successfully with oral Metronidazole and Vancomycin in the confirmed infected patients.
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spelling pubmed-86973102022-01-07 Toxigenic Clostridium difficile-mediated diarrhoea in hematopoietic stem cell transplantation in-patients: rapid diagnosis and efficient treatment Khodaparast, Sepideh Mohabati Mobarez, Ashraf Khoramabadi, Nima Vasei, Mohammad Saberifiroozi, Mehdi Epidemiol Infect Short Paper Allogenic hematopoietic stem cell transplant (HSCT) recipients are susceptible to any kind of infectious agents including Clostridium difficile. We studied 86 allogenic-HSCT patients who faced diarrhoea while receiving antibiotics. DNA from stool samples were explored for the presence of C. difficile toxin genes (tcdA; tcdB) by multiplex real-time PCR. Results showed nine toxigenic C. difficile amongst which seven were positive for both toxins and two were positive for tcdB. Six of toxigenic C. difficile organisms harbouring both toxin genes were also isolated by toxigenic culture. Clostridium difficile infection was controlled successfully with oral Metronidazole and Vancomycin in the confirmed infected patients. Cambridge University Press 2021-08-10 /pmc/articles/PMC8697310/ /pubmed/34372951 http://dx.doi.org/10.1017/S095026882100162X Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited.
spellingShingle Short Paper
Khodaparast, Sepideh
Mohabati Mobarez, Ashraf
Khoramabadi, Nima
Vasei, Mohammad
Saberifiroozi, Mehdi
Toxigenic Clostridium difficile-mediated diarrhoea in hematopoietic stem cell transplantation in-patients: rapid diagnosis and efficient treatment
title Toxigenic Clostridium difficile-mediated diarrhoea in hematopoietic stem cell transplantation in-patients: rapid diagnosis and efficient treatment
title_full Toxigenic Clostridium difficile-mediated diarrhoea in hematopoietic stem cell transplantation in-patients: rapid diagnosis and efficient treatment
title_fullStr Toxigenic Clostridium difficile-mediated diarrhoea in hematopoietic stem cell transplantation in-patients: rapid diagnosis and efficient treatment
title_full_unstemmed Toxigenic Clostridium difficile-mediated diarrhoea in hematopoietic stem cell transplantation in-patients: rapid diagnosis and efficient treatment
title_short Toxigenic Clostridium difficile-mediated diarrhoea in hematopoietic stem cell transplantation in-patients: rapid diagnosis and efficient treatment
title_sort toxigenic clostridium difficile-mediated diarrhoea in hematopoietic stem cell transplantation in-patients: rapid diagnosis and efficient treatment
topic Short Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8697310/
https://www.ncbi.nlm.nih.gov/pubmed/34372951
http://dx.doi.org/10.1017/S095026882100162X
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