Dual-signal lateral flow assay using vancomycin-modified nanotags for rapid and sensitive detection of Staphylococcus aureus

This paper reports a colorimetric-fluorescent dual-signal lateral flow assay (LFA) based on vancomycin (Van)-modified SiO(2)–Au-QD tags for sensitive and quantitative detection of Staphylococcus aureus (S. aureus). The combination of high-performance Van-tags and detection antibodies integrated into...

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Detalles Bibliográficos
Autores principales: Wang, Shu, Shen, Wanzhu, Zheng, Shuai, Li, Zhigang, Wang, Chongwen, Zhang, Long, Liu, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8697553/
https://www.ncbi.nlm.nih.gov/pubmed/35423879
http://dx.doi.org/10.1039/d1ra01085a
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author Wang, Shu
Shen, Wanzhu
Zheng, Shuai
Li, Zhigang
Wang, Chongwen
Zhang, Long
Liu, Yong
author_facet Wang, Shu
Shen, Wanzhu
Zheng, Shuai
Li, Zhigang
Wang, Chongwen
Zhang, Long
Liu, Yong
author_sort Wang, Shu
collection PubMed
description This paper reports a colorimetric-fluorescent dual-signal lateral flow assay (LFA) based on vancomycin (Van)-modified SiO(2)–Au-QD tags for sensitive and quantitative detection of Staphylococcus aureus (S. aureus). The combination of high-performance Van-tags and detection antibodies integrated into the LFA system produced assays with high sensitivity and specificity. The visualization limit of the colorimetric signal and the detection limit of the fluorescence signal of the proposed method for S. aureus can reach 10(4) and 100 cells mL(−1), respectively.
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spelling pubmed-86975532022-04-13 Dual-signal lateral flow assay using vancomycin-modified nanotags for rapid and sensitive detection of Staphylococcus aureus Wang, Shu Shen, Wanzhu Zheng, Shuai Li, Zhigang Wang, Chongwen Zhang, Long Liu, Yong RSC Adv Chemistry This paper reports a colorimetric-fluorescent dual-signal lateral flow assay (LFA) based on vancomycin (Van)-modified SiO(2)–Au-QD tags for sensitive and quantitative detection of Staphylococcus aureus (S. aureus). The combination of high-performance Van-tags and detection antibodies integrated into the LFA system produced assays with high sensitivity and specificity. The visualization limit of the colorimetric signal and the detection limit of the fluorescence signal of the proposed method for S. aureus can reach 10(4) and 100 cells mL(−1), respectively. The Royal Society of Chemistry 2021-04-09 /pmc/articles/PMC8697553/ /pubmed/35423879 http://dx.doi.org/10.1039/d1ra01085a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Wang, Shu
Shen, Wanzhu
Zheng, Shuai
Li, Zhigang
Wang, Chongwen
Zhang, Long
Liu, Yong
Dual-signal lateral flow assay using vancomycin-modified nanotags for rapid and sensitive detection of Staphylococcus aureus
title Dual-signal lateral flow assay using vancomycin-modified nanotags for rapid and sensitive detection of Staphylococcus aureus
title_full Dual-signal lateral flow assay using vancomycin-modified nanotags for rapid and sensitive detection of Staphylococcus aureus
title_fullStr Dual-signal lateral flow assay using vancomycin-modified nanotags for rapid and sensitive detection of Staphylococcus aureus
title_full_unstemmed Dual-signal lateral flow assay using vancomycin-modified nanotags for rapid and sensitive detection of Staphylococcus aureus
title_short Dual-signal lateral flow assay using vancomycin-modified nanotags for rapid and sensitive detection of Staphylococcus aureus
title_sort dual-signal lateral flow assay using vancomycin-modified nanotags for rapid and sensitive detection of staphylococcus aureus
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8697553/
https://www.ncbi.nlm.nih.gov/pubmed/35423879
http://dx.doi.org/10.1039/d1ra01085a
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