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Design of new bioinspired GO-COOH decorated alginate/gelatin hybrid scaffolds with nanofibrous architecture: structural, mechanical and biological investigations

The current research study deals with the design and investigation of novel bioinspired and biocompatible GO-COOH decorated hybrid polymeric scaffolds with nanofibrous architecture as biomaterials with highly appropriate features for functional restoration of damaged tissue. Gelatin and alginate, tw...

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Autores principales: Ghitman, Jana, Biru, Elena Iuliana, Cojocaru, Elena, Pircalabioru, Gratiela Gradisteanu, Vasile, Eugeniu, Iovu, Horia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8697576/
https://www.ncbi.nlm.nih.gov/pubmed/35423873
http://dx.doi.org/10.1039/d1ra01432c
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author Ghitman, Jana
Biru, Elena Iuliana
Cojocaru, Elena
Pircalabioru, Gratiela Gradisteanu
Vasile, Eugeniu
Iovu, Horia
author_facet Ghitman, Jana
Biru, Elena Iuliana
Cojocaru, Elena
Pircalabioru, Gratiela Gradisteanu
Vasile, Eugeniu
Iovu, Horia
author_sort Ghitman, Jana
collection PubMed
description The current research study deals with the design and investigation of novel bioinspired and biocompatible GO-COOH decorated hybrid polymeric scaffolds with nanofibrous architecture as biomaterials with highly appropriate features for functional restoration of damaged tissue. Gelatin and alginate, two biobased-polymers with excellent biocompatibility, high microenvironment biomimicry and ability for proper guidance of cell development in combination with carboxylated graphene oxide (GO-COOH), embody the matrix of electrospun hybrid scaffolds. The underlying principle is based on various types of interactions that can take place between the functionalities of the system's entities (proved by DLS) and their synergy in improving the structural integrity, mechanical tailorability and biological performances of the new nanofibrous GO-COOH decorated hybrid scaffolds. The nanofibrous structure along with the presence of GO-COOH are established by SEM. The new covalent bonds formed between various functionalities of the protein-polysaccharide-GO-COOH system are proved by FTIR and XPS. The physico-chemical state of GO-COOH lattices within the hybrid structures is investigated by Raman spectrometry. The interpenetrated network of bicomponent structures determines a 10-fold increase of Young's modulus as compared to monocomponent counterparts while the dispersion of GO-COOH significantly increases the elasticity of materials. The biological results (MTT and LDH assays) indicate a good cytocompatibility of crosslinked bicomponent AGS scaffolds; the metabolic cellular activity is substantially improved following the GO-COOH addition, suggesting that GO-COOH can support the cell adhesion, growth and proliferation.
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spelling pubmed-86975762022-04-13 Design of new bioinspired GO-COOH decorated alginate/gelatin hybrid scaffolds with nanofibrous architecture: structural, mechanical and biological investigations Ghitman, Jana Biru, Elena Iuliana Cojocaru, Elena Pircalabioru, Gratiela Gradisteanu Vasile, Eugeniu Iovu, Horia RSC Adv Chemistry The current research study deals with the design and investigation of novel bioinspired and biocompatible GO-COOH decorated hybrid polymeric scaffolds with nanofibrous architecture as biomaterials with highly appropriate features for functional restoration of damaged tissue. Gelatin and alginate, two biobased-polymers with excellent biocompatibility, high microenvironment biomimicry and ability for proper guidance of cell development in combination with carboxylated graphene oxide (GO-COOH), embody the matrix of electrospun hybrid scaffolds. The underlying principle is based on various types of interactions that can take place between the functionalities of the system's entities (proved by DLS) and their synergy in improving the structural integrity, mechanical tailorability and biological performances of the new nanofibrous GO-COOH decorated hybrid scaffolds. The nanofibrous structure along with the presence of GO-COOH are established by SEM. The new covalent bonds formed between various functionalities of the protein-polysaccharide-GO-COOH system are proved by FTIR and XPS. The physico-chemical state of GO-COOH lattices within the hybrid structures is investigated by Raman spectrometry. The interpenetrated network of bicomponent structures determines a 10-fold increase of Young's modulus as compared to monocomponent counterparts while the dispersion of GO-COOH significantly increases the elasticity of materials. The biological results (MTT and LDH assays) indicate a good cytocompatibility of crosslinked bicomponent AGS scaffolds; the metabolic cellular activity is substantially improved following the GO-COOH addition, suggesting that GO-COOH can support the cell adhesion, growth and proliferation. The Royal Society of Chemistry 2021-04-13 /pmc/articles/PMC8697576/ /pubmed/35423873 http://dx.doi.org/10.1039/d1ra01432c Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Ghitman, Jana
Biru, Elena Iuliana
Cojocaru, Elena
Pircalabioru, Gratiela Gradisteanu
Vasile, Eugeniu
Iovu, Horia
Design of new bioinspired GO-COOH decorated alginate/gelatin hybrid scaffolds with nanofibrous architecture: structural, mechanical and biological investigations
title Design of new bioinspired GO-COOH decorated alginate/gelatin hybrid scaffolds with nanofibrous architecture: structural, mechanical and biological investigations
title_full Design of new bioinspired GO-COOH decorated alginate/gelatin hybrid scaffolds with nanofibrous architecture: structural, mechanical and biological investigations
title_fullStr Design of new bioinspired GO-COOH decorated alginate/gelatin hybrid scaffolds with nanofibrous architecture: structural, mechanical and biological investigations
title_full_unstemmed Design of new bioinspired GO-COOH decorated alginate/gelatin hybrid scaffolds with nanofibrous architecture: structural, mechanical and biological investigations
title_short Design of new bioinspired GO-COOH decorated alginate/gelatin hybrid scaffolds with nanofibrous architecture: structural, mechanical and biological investigations
title_sort design of new bioinspired go-cooh decorated alginate/gelatin hybrid scaffolds with nanofibrous architecture: structural, mechanical and biological investigations
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8697576/
https://www.ncbi.nlm.nih.gov/pubmed/35423873
http://dx.doi.org/10.1039/d1ra01432c
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