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Spatial correlation of cell stiffness and traction forces in cancer cells measured with combined SICM and TFM

The mechanical properties of cancer cells at the single-cell and the subcellular level might be the key for answering long-standing questions in the diagnosis and treatment of cancer. However, the subcellular distribution of two main mechanical properties, cell stiffness and traction forces, has bee...

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Detalles Bibliográficos
Autores principales: Rheinlaender, Johannes, Wirbel, Hannes, Schäffer, Tilman E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8697701/
https://www.ncbi.nlm.nih.gov/pubmed/35423943
http://dx.doi.org/10.1039/d1ra01277k
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author Rheinlaender, Johannes
Wirbel, Hannes
Schäffer, Tilman E.
author_facet Rheinlaender, Johannes
Wirbel, Hannes
Schäffer, Tilman E.
author_sort Rheinlaender, Johannes
collection PubMed
description The mechanical properties of cancer cells at the single-cell and the subcellular level might be the key for answering long-standing questions in the diagnosis and treatment of cancer. However, the subcellular distribution of two main mechanical properties, cell stiffness and traction forces, has been investigated only rarely and qualitatively yet. Here, we present the first direct combination of scanning ion conductance microscopy (SICM) and traction force microscopy (TFM), which we used to identify a correlation between the local stiffness and the local traction force density in living cells. We found a correlation in normal breast epithelial cells, but no correlation in cancerous breast epithelial cells. This indicates that the interplay between cell stiffness and traction forces is altered in cancer cells as compared to healthy cells, which might give new insight in the research field of cancer cell mechanobiology.
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spelling pubmed-86977012022-04-13 Spatial correlation of cell stiffness and traction forces in cancer cells measured with combined SICM and TFM Rheinlaender, Johannes Wirbel, Hannes Schäffer, Tilman E. RSC Adv Chemistry The mechanical properties of cancer cells at the single-cell and the subcellular level might be the key for answering long-standing questions in the diagnosis and treatment of cancer. However, the subcellular distribution of two main mechanical properties, cell stiffness and traction forces, has been investigated only rarely and qualitatively yet. Here, we present the first direct combination of scanning ion conductance microscopy (SICM) and traction force microscopy (TFM), which we used to identify a correlation between the local stiffness and the local traction force density in living cells. We found a correlation in normal breast epithelial cells, but no correlation in cancerous breast epithelial cells. This indicates that the interplay between cell stiffness and traction forces is altered in cancer cells as compared to healthy cells, which might give new insight in the research field of cancer cell mechanobiology. The Royal Society of Chemistry 2021-04-14 /pmc/articles/PMC8697701/ /pubmed/35423943 http://dx.doi.org/10.1039/d1ra01277k Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Rheinlaender, Johannes
Wirbel, Hannes
Schäffer, Tilman E.
Spatial correlation of cell stiffness and traction forces in cancer cells measured with combined SICM and TFM
title Spatial correlation of cell stiffness and traction forces in cancer cells measured with combined SICM and TFM
title_full Spatial correlation of cell stiffness and traction forces in cancer cells measured with combined SICM and TFM
title_fullStr Spatial correlation of cell stiffness and traction forces in cancer cells measured with combined SICM and TFM
title_full_unstemmed Spatial correlation of cell stiffness and traction forces in cancer cells measured with combined SICM and TFM
title_short Spatial correlation of cell stiffness and traction forces in cancer cells measured with combined SICM and TFM
title_sort spatial correlation of cell stiffness and traction forces in cancer cells measured with combined sicm and tfm
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8697701/
https://www.ncbi.nlm.nih.gov/pubmed/35423943
http://dx.doi.org/10.1039/d1ra01277k
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