The Efficiency of Probiotics Administrated via Different Routes and Doses in Enhancing Production Performance, Meat Quality, Gut Morphology, and Microbial Profile of Broiler Chickens

SIMPLE SUMMARY: Antimicrobial growth promoters (AGPs) have been used in the animal production industry around the world for decades, with the consequence of a high potential of antibiotic-resistant bacteria transfer to humans. Efficiently raising broiler chickens in an antibiotic-free production system is a challenge, and identifying an effective nutritional alternative to support growth performance, gut health, and functionality without administrating AGPs is of essence. Several antimicrobial alternative options that are commercially available include herbal essential oils, exogenous enzymes, organic acids, plant secondary metabolites, probiotics, and prebiotics. Probiotics in animal feed is projected to attain a massive global growth, reaching USD 6.24 billion by 2026. This study tested the efficiency of probiotics when supplemented via different administration routes (feed or water) and doses, or in combination with prebiotics, on growth performance, meat quality, gut morphology, and microbial profile of broiler chickens. The outcomes revealed that probiotics enhance production performance, and compared to AGPs, do not reduce the beta-diversity of the gut microbial community. Water-soluble probiotics seemed to be more effective in improving growth performance. ABSTRACT: To study the efficiency of Bacillus spp. probiotics administered via different routes and doses, a 6-week grow-out trial was conducted using a total of 378 day-old mixed-sex ROSS308 broiler chickens in a completely randomized block design. Six experimental diets included probiotics added at two different inclusion rates into the feed (250 g/ton; PRO250, or 500 g/ton; PRO500), or in the drinking water (25 g/L; PRO-WS), or as a feed synbiotic (250 g probiotic + 250 g/ton prebiotic; SYN), compared to a negative (NC; without additives) and positive control (PC; with antibiotics) diets. The PRO-WS enhanced feed intake (p < 0.05) and tended to improve average daily gain and final body weight (p = 0.14). Broiler gut morphology in the duodenum including the villus height (p = 0.04), villus width (p = 0.05) and crypt depth (p = 0.02) were improved by PRO500. Firmicutes was the most abundant phylum, followed by Bacteroidetes. Streptococcaceae, Lachnoospiraceae, Peptostreptococcaceae, Ruminococcaceae, and Erysipe-lotrichaceae were the top five most abundant families. Antibiotic inclusion in PC reduced microbial beta-diversity and increased similarity compared to probiotic inclusion (p = 0.05). Probiotic inclusion reduced the relative abundance of Bacteroides fragilis, which is a commonly isolated pathogen and is considered as a marker for antimicrobial resistance. Overall, probiotic supplementation via feed or water may potentially improve the production performance of the broiler chickens, and water-soluble probiotics are potentially more effective. Probiotics, especially when added to water, suggest a promising feed additive to support gut microbial maturation and diversity, and may reduce resistant bacteria in broiler chickens. However, it is suggested that the best route for the administration of probiotics be further examined under commercial conditions to find the most effective and practical application method that yields the most consistent results.