Effects of Dietary Supplementation with Mushroom or Vitamin D(2)-Enriched Mushroom Powders on Gastrointestinal Health Parameters in the Weaned Pig

SIMPLE SUMMARY: The prospective ban on zinc oxide in pig feed in Europe is a major challenge facing the swine industry to maintain piglet health and performance during the weaning period. Weaning is a particularly difficult period for the young pig that is associated with abrupt dietary, environmental and social changes that cause significant levels of stress and disrupt gut development in the pig. Mushrooms are a rich natural source of bio-actives and have long been regarded as a health-promoting food due to their immunomodulatory and antioxidant effects and their ability to modulate the gut microbiota. Mushrooms contain high levels of ergosterol, which allows them to naturally produce vitamin D when they are exposed to light. The present study aimed to compare the effects of mushroom and vitamin D(2)-enriched mushroom powders to zinc oxide on the molecular, physiological and microbial changes that influence performance during the post-weaning period. Our study showed that vitamin D(2)-enriched mushrooms were equally as effective as zinc oxide in improving gastrointestinal health parameters. However, both mushroom powders reduced feed intake in pigs and negatively affected animal performance. For this reason, mushroom powders have limited use as a commercial feed additive in replacing zinc oxide in pig diets. ABSTRACT: The objective of this study was to compare the molecular, physiological and microbial effects of mushroom powder (MP), vitamin D(2) enriched mushroom powder (MPD(2)) and zinc oxide (ZnO) in pigs post-weaning. Pigs (four pigs/pen; 12 pens/treatment) were assigned to: (1) basal diet (control), (2) basal diet + ZnO, (3) basal diet + MP (2 g/kg feed) and (4) basal diet + MPD(2) (2 g/kg feed). Zinc oxide supplementation improved the feed intake (p < 0.001); increased the caecal abundance of Lactobacillus (p < 0.05); increased the villus height (p < 0.05) in the duodenum, jejunum and ileum; increased the expression of chemokine interleukin 8 (CXCL8; p < 0.05); and decreased the expression of pro-inflammatory cytokine gene interleukin 6 (IL6; p < 0.05), tumour necrosis factor (TNF; p < 0.05), nutrient transporters peptide transporter 1 (SLC15A1; p < 0.05) and fatty acid binding protein 2 (FABP2; (p < 0.05) in the duodenum. Whereas dietary supplementation with MPD(2) improved the gastrointestinal morphology (p < 0.05); increased the total volatile fatty acid concentrations (p < 0.05); increased the expression of anti-inflammatory cytokine gene interleukin 10 (IL10; p < 0.05) and nutrient transporters SLC15A1 (p < 0.05), FABP2 (p < 0.05) and vitamin D receptor (VDR; p < 0.05); and reduced the expression of pro-inflammatory cytokine gene IL6 (p < 0.05), it adversely affected average daily feed intake (ADFI; p < 0.001) and average daily gain (ADG; p < 0.05). Mushroom powder supplementation had a positive impact on gastrointestinal morphology (p < 0.05) and upregulated the expression of nutrient transporters SLC15A1 (p < 0.05) and FABP2 (p < 0.05) and tight junction claudin 1 (CLDN1) (p < 0.05) compared to the controls but had no effect on the expression of inflammatory markers (p > 0.05). Furthermore, MP reduced ADFI (p < 0.01); however, this did not negatively impact the ADG (p > 0.05). In conclusion, MP and MPD(2) have limited use as commercial feed additives in replacing ZnO in pig diets as feed intake was reduced post-weaning.