Evaluation of Fermented Oat and Black Soldier Fly Larva as Food Ingredients in Senior Dog Diets

SIMPLE SUMMARY: Along with concerns about the shortage of future food resources, the problem of ensuring a stable supply of feed materials is emerging. The rapid growth of the pet food market is also increasing the demand for new food ingredients, requiring the evaluation of their safety and nutritional value. Recently, insects and fermented foods are some of the materials that have entered the spotlight as potential future foods, and studies on their usefulness as food are being actively conducted. This study aimed to evaluate and verify the safety of fermented oat (Avena sativa) and black soldier fly larva (Hermetia illucens L.) when used in a dog food as part of the effort toward discovering suitable nutritionally excellent and functional food materials. Our results show that 10% fermented oat flour, 5% black soldier fly larva meal, or a combination thereof in the food did not negatively affect food intake, body weight, fecal status, skin condition, or hematological and biochemical parameters. Overall, our findings suggest that fermented oat and black soldier fly larva can be used as food ingredients for dogs. ABSTRACT: The aim of this study was to evaluate the suitability of fermented oat (FO) and black soldier fly larva (BSFL) as food ingredients for dogs. A total of 20 spayed female dogs were divided into four treatment groups, with 5 dogs per group. The four treatment groups consisted of a control group, a diet with 10% FO, one with 5% BSFL, and one with 10% FO and 5% BSFL, and each experimental food was fed for 12 weeks. The feeding of FO and/or BSFL did not affect the daily food intake, body weight, body condition score, fecal score, or skin condition of the dogs. In all the experimental groups, no significant differences in serum IgG, IL-10, or TNF-α levels were observed upon the feeding of FO and/or BSFL. Some hematological (white blood cell and basophils) and serum biochemical parameters (phosphorous, globulin, and alkaline phosphatase) showed significant differences with FO and/or BSFL feeding compared to the control group, but they were within the normal reference range. No adverse clinical signs related to these parameters being affected by FO and BSFL were observed. The feeding of BSFL for 12 weeks reduced the serum cholesterol level (p < 0.05) at the end of the experiment. Our findings suggest the suitability of FO and BSFL as food materials for dogs.