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In Silico Analysis of PKS and NRPS Gene Clusters in Arisostatin- and Kosinostatin-Producers and Description of Micromonospora okii sp. nov.

Micromonospora sp. TP-A0316 and Micromonospora sp. TP-A0468 are producers of arisostatin and kosinostatin, respectively. Micromonospora sp. TP-A0316 showed a 16S rRNA gene sequence similarity of 100% to Micromonospora oryzae CP2R9-1(T) whereas Micromonospora sp. TP-A0468 showed a 99.3% similarity to...

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Autores principales: Komaki, Hisayuki, Ichikawa, Natsuko, Hosoyama, Akira, Hamada, Moriyuki, Igarashi, Yasuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8698034/
https://www.ncbi.nlm.nih.gov/pubmed/34943659
http://dx.doi.org/10.3390/antibiotics10121447
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author Komaki, Hisayuki
Ichikawa, Natsuko
Hosoyama, Akira
Hamada, Moriyuki
Igarashi, Yasuhiro
author_facet Komaki, Hisayuki
Ichikawa, Natsuko
Hosoyama, Akira
Hamada, Moriyuki
Igarashi, Yasuhiro
author_sort Komaki, Hisayuki
collection PubMed
description Micromonospora sp. TP-A0316 and Micromonospora sp. TP-A0468 are producers of arisostatin and kosinostatin, respectively. Micromonospora sp. TP-A0316 showed a 16S rRNA gene sequence similarity of 100% to Micromonospora oryzae CP2R9-1(T) whereas Micromonospora sp. TP-A0468 showed a 99.3% similarity to Micromonospora haikouensis 232617(T). A phylogenetic analysis based on gyrB sequences suggested that Micromonospora sp. TP-A0316 is closely related to Micromonospora oryzae whereas Micromonospora TP-A0468 is an independent genomospecies. As Micromonospora sp. TP-A0468 showed some phenotypic differences to its closely related species, it was classified as a novel species, for which the name Micromonospora okii sp. nov. is proposed. The type strain is TP-A0468(T) (= NBRC 110461(T)). Micromonospora sp. TP-A0316 and M. okii TP-A0468(T) were both found to harbor 15 gene clusters for secondary metabolites such as polyketides and nonribosomal peptides in their genomes. Arisostatin-biosynthetic gene cluster (BGC) of Micromonospora sp. TP-A0316 closely resembled tetrocarcin A-BGC of Micromonospora chalcea NRRL 11289. A large type-I polyketide synthase gene cluster was present in each genome of Micromonospora sp. TP-A0316 and M. okii TP-A0468(T). It was an ortholog of quinolidomicin-BGC of M. chalcea AK-AN57 and widely distributed in the genus Micromonospora.
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spelling pubmed-86980342021-12-24 In Silico Analysis of PKS and NRPS Gene Clusters in Arisostatin- and Kosinostatin-Producers and Description of Micromonospora okii sp. nov. Komaki, Hisayuki Ichikawa, Natsuko Hosoyama, Akira Hamada, Moriyuki Igarashi, Yasuhiro Antibiotics (Basel) Article Micromonospora sp. TP-A0316 and Micromonospora sp. TP-A0468 are producers of arisostatin and kosinostatin, respectively. Micromonospora sp. TP-A0316 showed a 16S rRNA gene sequence similarity of 100% to Micromonospora oryzae CP2R9-1(T) whereas Micromonospora sp. TP-A0468 showed a 99.3% similarity to Micromonospora haikouensis 232617(T). A phylogenetic analysis based on gyrB sequences suggested that Micromonospora sp. TP-A0316 is closely related to Micromonospora oryzae whereas Micromonospora TP-A0468 is an independent genomospecies. As Micromonospora sp. TP-A0468 showed some phenotypic differences to its closely related species, it was classified as a novel species, for which the name Micromonospora okii sp. nov. is proposed. The type strain is TP-A0468(T) (= NBRC 110461(T)). Micromonospora sp. TP-A0316 and M. okii TP-A0468(T) were both found to harbor 15 gene clusters for secondary metabolites such as polyketides and nonribosomal peptides in their genomes. Arisostatin-biosynthetic gene cluster (BGC) of Micromonospora sp. TP-A0316 closely resembled tetrocarcin A-BGC of Micromonospora chalcea NRRL 11289. A large type-I polyketide synthase gene cluster was present in each genome of Micromonospora sp. TP-A0316 and M. okii TP-A0468(T). It was an ortholog of quinolidomicin-BGC of M. chalcea AK-AN57 and widely distributed in the genus Micromonospora. MDPI 2021-11-25 /pmc/articles/PMC8698034/ /pubmed/34943659 http://dx.doi.org/10.3390/antibiotics10121447 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Komaki, Hisayuki
Ichikawa, Natsuko
Hosoyama, Akira
Hamada, Moriyuki
Igarashi, Yasuhiro
In Silico Analysis of PKS and NRPS Gene Clusters in Arisostatin- and Kosinostatin-Producers and Description of Micromonospora okii sp. nov.
title In Silico Analysis of PKS and NRPS Gene Clusters in Arisostatin- and Kosinostatin-Producers and Description of Micromonospora okii sp. nov.
title_full In Silico Analysis of PKS and NRPS Gene Clusters in Arisostatin- and Kosinostatin-Producers and Description of Micromonospora okii sp. nov.
title_fullStr In Silico Analysis of PKS and NRPS Gene Clusters in Arisostatin- and Kosinostatin-Producers and Description of Micromonospora okii sp. nov.
title_full_unstemmed In Silico Analysis of PKS and NRPS Gene Clusters in Arisostatin- and Kosinostatin-Producers and Description of Micromonospora okii sp. nov.
title_short In Silico Analysis of PKS and NRPS Gene Clusters in Arisostatin- and Kosinostatin-Producers and Description of Micromonospora okii sp. nov.
title_sort in silico analysis of pks and nrps gene clusters in arisostatin- and kosinostatin-producers and description of micromonospora okii sp. nov.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8698034/
https://www.ncbi.nlm.nih.gov/pubmed/34943659
http://dx.doi.org/10.3390/antibiotics10121447
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