Influence of Haemolysis on the Mineral Profile of Cattle Serum

SIMPLE SUMMARY: The results of blood tests routinely used in clinical chemistry can be altered by haemolysis, the disruption of red blood cells. Haemolysis of serum samples is recognized to be the leading cause of preanalytical errors in clinical laboratories. The influence of haemolysis must be specifically studied for each analyte and species of clinical interest, as it is often not known how serum samples are affected. Little is known about the potential alterations in the concentrations of mineral elements in haemolyzed serum in general and the phenomenon has not been specifically studied in bovine serum samples. We investigate how haemolysis affects the mineral content of bovine samples. ABSTRACT: Haemolysis of serum samples is the leading cause of preanalytical errors in clinical laboratories. Little is known about the potential alterations in the concentrations of mineral elements in haemolyzed serum and the phenomenon has not been specifically studied in bovine serum samples. We investigate how haemolysis affects the mineral content of bovine samples. We used ICP-MS to measure the concentrations of 12 mineral elements (Ca, Co, Cr, Cu, Fe, Mg, Mn, Mo, Ni, P, Se and Zn) in bovine whole blood, serum and gradually haemolyzed samples and observed significant differences between the different types of samples, particularly in the Fe and Zn concentrations. However, in practice, the high interindividual variability makes it difficult to establish whether a given value corresponds to normal or haemolyzed samples. In response to this problem, we propose to consider that a result is significantly biased when the haemolysis threshold (the degree of haemolysis above which the concentration of an element in serum is significantly altered) of a given element is surpassed. The haemolysis threshold values for the different elements considered were found as follows: 0.015 g Hb L(−1) for Fe, 2 g for Zn, 4 g for Cr and 8 g for Ca, Se and Mo.