Rearing of Bitterling (Rhodeus amarus) Larvae and Fry under Controlled Conditions for the Restitution of Endangered Populations

SIMPLE SUMMARY: Among the many species threatened with extinction and covered by protection of species is bitterling Rhodeus amarus. It belongs to ostracophilic fish that place spawn inside live mussels. Bitterlings, such as mussels, belongs to indicator species that testify to the good state of the natural environment. Supporting the populations of these organisms is a necessity in order to preserve the biodiversity of inland waters, which are subjected to severe anthropopression. The development in advance of a method of reproduction and breeding of bitterling under controlled conditions can ensure their survival in the event of an imbalance in the natural environment. These were the first studies of this type, where after 6.5 months of rearing, sexually mature individuals were obtained. In this way, a suitable stocking material of the bitterling was obtained in order to save the endangered populations. ABSTRACT: Among the several dozen European freshwater fish species, only European bitterling (Rhodeus amarus Bloch) and Rhodeus meridionalis belong to the group of ostrakophilous fish. The embryonic and larval development of the fish in this reproductive group until the time of the yolk sac resorption takes place in the gill cavity of river mussels (Anodonta sp. or Unio sp.). This paper presents the results of the European bitterling Rhodeus amarus being reared under controlled conditions. Bitterling larvae were caught together with river mussels in the natural environment and subsequently placed in a tank for behavioural observations. Bitterling larvae were seen swimming in the water within a week of placing the bivalves under controlled conditions. The bitterling larvae were 8.6 ± 0.11 mm long when they started to swim actively. The rearing was conducted in water at 20 and 26 ± 0.5 °C and lasted for 6.5 months (200 days) in both variants. Initially, the larvae were fed with live nauplii of Artemia salina and subsequently with fodder. The bitterlings in tanks with water at 26 ± 0.5 °C were 66.2 ± 3.0 mm long and weighed 3389 ± 548 mg. For comparison, bitterlings kept in water at 20 ± 0.5 °C were 64.48 ± 3.4 mm long and weighed 3242 ± 427 mg. No larval malformities or mortality were observed during the larvae and fry rearing. The bitterlings had well-developed secondary sexual characteristics and exhibited pre-spawning behaviour at the end of the rearing. This produced suitable bitterling stocking material to be used in the conservation of small or endangered populations.