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Clinical Features and Molecular Characteristics of Methicillin-Susceptible Staphylococcus aureus Ocular Infection in Taiwan

This study analyzed the clinical features and molecular characteristics of methicillin-susceptible Staphylococcus aureus (MSSA) ocular infections in Taiwan and compared them between community-associated (CA) and health-care-associated (HA) infections. We collected S. aureus ocular isolates from pati...

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Autores principales: Chen, Yueh-Ling, Kang, Eugene Yu-Chuan, Yeh, Lung-Kun, Ma, David H. K., Tan, Hsin-Yuan, Chen, Hung-Chi, Hung, Kuo-Hsuan, Huang, Yhu-Chering, Hsiao, Ching-Hsi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8698105/
https://www.ncbi.nlm.nih.gov/pubmed/34943657
http://dx.doi.org/10.3390/antibiotics10121445
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author Chen, Yueh-Ling
Kang, Eugene Yu-Chuan
Yeh, Lung-Kun
Ma, David H. K.
Tan, Hsin-Yuan
Chen, Hung-Chi
Hung, Kuo-Hsuan
Huang, Yhu-Chering
Hsiao, Ching-Hsi
author_facet Chen, Yueh-Ling
Kang, Eugene Yu-Chuan
Yeh, Lung-Kun
Ma, David H. K.
Tan, Hsin-Yuan
Chen, Hung-Chi
Hung, Kuo-Hsuan
Huang, Yhu-Chering
Hsiao, Ching-Hsi
author_sort Chen, Yueh-Ling
collection PubMed
description This study analyzed the clinical features and molecular characteristics of methicillin-susceptible Staphylococcus aureus (MSSA) ocular infections in Taiwan and compared them between community-associated (CA) and health-care-associated (HA) infections. We collected S. aureus ocular isolates from patients at Chang Gung Memorial Hospital between 2010 and 2017. The infections were classified as CA or HA using epidemiological criteria, and the isolates were molecularly characterized using pulsed-field gel electrophoresis, multilocus sequence typing, and Panton-Valentine leukocidin (PVL) gene detection. Antibiotic susceptibility was evaluated using disk diffusion and an E test. A total of 104 MSSA ocular isolates were identified; 46 (44.2%) were CA-MSSA and 58 (55.8%) were HA-MSSA. Compared with HA-MSSA strains, CA-MSSA strains caused a significantly higher rate of keratitis, but a lower rate of conjunctivitis. We identified 14 pulsotypes. ST 7/pulsotype BA was frequently identified in both CA-MSSA (28.3%) and HA-MSSA (37.9%) cases. PVL genes were identified in seven isolates (6.7%). Both CA-MSSA and HA-MSSA isolates were highly susceptible to vancomycin, teicoplanin, tigecycline, sulfamethoxazole–trimethoprim, and fluoroquinolones. The most common ocular manifestations were keratitis and conjunctivitis for CA-MSSA and HA-MSSA, respectively. The MSSA ocular isolates had diverse molecular characteristics; no specific genotype differentiated CA-MSSA from HA-MSSA. Both strains exhibited similar antibiotic susceptibility.
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spelling pubmed-86981052021-12-24 Clinical Features and Molecular Characteristics of Methicillin-Susceptible Staphylococcus aureus Ocular Infection in Taiwan Chen, Yueh-Ling Kang, Eugene Yu-Chuan Yeh, Lung-Kun Ma, David H. K. Tan, Hsin-Yuan Chen, Hung-Chi Hung, Kuo-Hsuan Huang, Yhu-Chering Hsiao, Ching-Hsi Antibiotics (Basel) Article This study analyzed the clinical features and molecular characteristics of methicillin-susceptible Staphylococcus aureus (MSSA) ocular infections in Taiwan and compared them between community-associated (CA) and health-care-associated (HA) infections. We collected S. aureus ocular isolates from patients at Chang Gung Memorial Hospital between 2010 and 2017. The infections were classified as CA or HA using epidemiological criteria, and the isolates were molecularly characterized using pulsed-field gel electrophoresis, multilocus sequence typing, and Panton-Valentine leukocidin (PVL) gene detection. Antibiotic susceptibility was evaluated using disk diffusion and an E test. A total of 104 MSSA ocular isolates were identified; 46 (44.2%) were CA-MSSA and 58 (55.8%) were HA-MSSA. Compared with HA-MSSA strains, CA-MSSA strains caused a significantly higher rate of keratitis, but a lower rate of conjunctivitis. We identified 14 pulsotypes. ST 7/pulsotype BA was frequently identified in both CA-MSSA (28.3%) and HA-MSSA (37.9%) cases. PVL genes were identified in seven isolates (6.7%). Both CA-MSSA and HA-MSSA isolates were highly susceptible to vancomycin, teicoplanin, tigecycline, sulfamethoxazole–trimethoprim, and fluoroquinolones. The most common ocular manifestations were keratitis and conjunctivitis for CA-MSSA and HA-MSSA, respectively. The MSSA ocular isolates had diverse molecular characteristics; no specific genotype differentiated CA-MSSA from HA-MSSA. Both strains exhibited similar antibiotic susceptibility. MDPI 2021-11-25 /pmc/articles/PMC8698105/ /pubmed/34943657 http://dx.doi.org/10.3390/antibiotics10121445 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Yueh-Ling
Kang, Eugene Yu-Chuan
Yeh, Lung-Kun
Ma, David H. K.
Tan, Hsin-Yuan
Chen, Hung-Chi
Hung, Kuo-Hsuan
Huang, Yhu-Chering
Hsiao, Ching-Hsi
Clinical Features and Molecular Characteristics of Methicillin-Susceptible Staphylococcus aureus Ocular Infection in Taiwan
title Clinical Features and Molecular Characteristics of Methicillin-Susceptible Staphylococcus aureus Ocular Infection in Taiwan
title_full Clinical Features and Molecular Characteristics of Methicillin-Susceptible Staphylococcus aureus Ocular Infection in Taiwan
title_fullStr Clinical Features and Molecular Characteristics of Methicillin-Susceptible Staphylococcus aureus Ocular Infection in Taiwan
title_full_unstemmed Clinical Features and Molecular Characteristics of Methicillin-Susceptible Staphylococcus aureus Ocular Infection in Taiwan
title_short Clinical Features and Molecular Characteristics of Methicillin-Susceptible Staphylococcus aureus Ocular Infection in Taiwan
title_sort clinical features and molecular characteristics of methicillin-susceptible staphylococcus aureus ocular infection in taiwan
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8698105/
https://www.ncbi.nlm.nih.gov/pubmed/34943657
http://dx.doi.org/10.3390/antibiotics10121445
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