Effects of Multimodal Analgesic Protocol, with Buprenorphine and Meloxicam, on Mice Well-Being: A Dose Finding Study

SIMPLE SUMMARY: Inadequate pain management affects animal welfare and scientific data validity. Multimodal analgesia is effective in reducing postoperative pain. However, surgery-related pain is not the only distress animals suffer during the perioperative period. The anesthetic or analgesic agent of choice, route and frequency of anesthetic or analgesic administration, and stressors such as anxiety and fear also induce distress. We hypothesized that a multimodal analgesic protocol using buprenorphine and meloxicam would have analgesic effects, and evaluated the effects of methods and drugs used for anesthesia and analgesia on the well-being of mice assigned to different groups. Even in the absence of surgical pain, the anesthesia + analgesia group presented the same negative effects as the surgery + anesthesia + analgesia group. The multimodal analgesic protocol, using buprenorphine and meloxicam, for mice is expected to have an analgesic effect on pain associated with laparotomy but was not sufficient in preventing food intake and weight decrease. This does not negate the need to administer analgesics, but suggests the need to focus on and care not only about the approach to relieve pain associated with surgery, but also other types of distresses in order to minimize negative side effects that may interfere with postoperative recovery in mice. ABSTRACT: The anesthetic or analgesic agent of choice, route and frequency of anesthetic or analgesic administration, and stressors induce distress during the perioperative period. We evaluated a multimodal analgesic protocol using buprenorphine and meloxicam on the well-being of mice. Twenty-four Slc:ICR male mice were divided into control, anesthesia + analgesia, and surgery + anesthesia + analgesia groups. Tap water (orally: PO) and water for injection (subcutaneous: SC) were administered to the control group. Buprenorphine was administered twice (SC, 0.1 mg/kg/8 h) and meloxicam was administered thrice (PO, 5 mg/kg/24 h) to the anesthesia + analgesia and surgery + anesthesia + analgesia groups. The mice were subjected to laparotomy and assessed for several parameters. Even in absence of surgical pain, the anesthesia + analgesia group presented the same negative effects as the surgery + anesthesia + analgesia group. This multimodal analgesic protocol for mice was expected to have an analgesic effect on pain associated with laparotomy but was not sufficient to prevent food intake and weight decrease. This does not negate the need to administer analgesics, but suggests the need to focus on and care not only about the approach to relieve pain associated with surgery, but also other types of distresses to minimize negative side effects that may interfere with postoperative recovery in mice.