Cargando…

Vitamin D Prevents High Glucose-Induced Lipid Droplets Accumulation in Cultured Endothelial Cells: The Role of Thioredoxin Interacting Protein

Vitamin D (VitD) exerts protective effects on the endothelium, which is fundamental for vascular integrity, partly by inhibiting free radical formation. We found that VitD prevents high glucose-induced Thioredoxin Interacting Protein (TXNIP) upregulation. Increased amounts of TXNIP are responsible f...

Descripción completa

Detalles Bibliográficos
Autores principales: Scrimieri, Roberta, Cazzaniga, Alessandra, Castiglioni, Sara, Maier, Jeanette A. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8698366/
https://www.ncbi.nlm.nih.gov/pubmed/34944690
http://dx.doi.org/10.3390/biomedicines9121874
_version_ 1784620259721871360
author Scrimieri, Roberta
Cazzaniga, Alessandra
Castiglioni, Sara
Maier, Jeanette A. M.
author_facet Scrimieri, Roberta
Cazzaniga, Alessandra
Castiglioni, Sara
Maier, Jeanette A. M.
author_sort Scrimieri, Roberta
collection PubMed
description Vitamin D (VitD) exerts protective effects on the endothelium, which is fundamental for vascular integrity, partly by inhibiting free radical formation. We found that VitD prevents high glucose-induced Thioredoxin Interacting Protein (TXNIP) upregulation. Increased amounts of TXNIP are responsible for the accumulation of reactive oxygen species and, as a consequence, of lipid droplets. This is associated with increased amounts of triglycerides as the result of increased lipogenesis and reduced fatty acid oxidation. Remarkably, VitD rebalances the redox equilibrium, restores normal lipid content, and prevents the accumulation of lipid droplets. Our results highlight TXNIP as one of the targets of VitD in high glucose-cultured endothelial cells and shed some light on the protective effect of VitD on the endothelium.
format Online
Article
Text
id pubmed-8698366
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-86983662021-12-24 Vitamin D Prevents High Glucose-Induced Lipid Droplets Accumulation in Cultured Endothelial Cells: The Role of Thioredoxin Interacting Protein Scrimieri, Roberta Cazzaniga, Alessandra Castiglioni, Sara Maier, Jeanette A. M. Biomedicines Article Vitamin D (VitD) exerts protective effects on the endothelium, which is fundamental for vascular integrity, partly by inhibiting free radical formation. We found that VitD prevents high glucose-induced Thioredoxin Interacting Protein (TXNIP) upregulation. Increased amounts of TXNIP are responsible for the accumulation of reactive oxygen species and, as a consequence, of lipid droplets. This is associated with increased amounts of triglycerides as the result of increased lipogenesis and reduced fatty acid oxidation. Remarkably, VitD rebalances the redox equilibrium, restores normal lipid content, and prevents the accumulation of lipid droplets. Our results highlight TXNIP as one of the targets of VitD in high glucose-cultured endothelial cells and shed some light on the protective effect of VitD on the endothelium. MDPI 2021-12-10 /pmc/articles/PMC8698366/ /pubmed/34944690 http://dx.doi.org/10.3390/biomedicines9121874 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Scrimieri, Roberta
Cazzaniga, Alessandra
Castiglioni, Sara
Maier, Jeanette A. M.
Vitamin D Prevents High Glucose-Induced Lipid Droplets Accumulation in Cultured Endothelial Cells: The Role of Thioredoxin Interacting Protein
title Vitamin D Prevents High Glucose-Induced Lipid Droplets Accumulation in Cultured Endothelial Cells: The Role of Thioredoxin Interacting Protein
title_full Vitamin D Prevents High Glucose-Induced Lipid Droplets Accumulation in Cultured Endothelial Cells: The Role of Thioredoxin Interacting Protein
title_fullStr Vitamin D Prevents High Glucose-Induced Lipid Droplets Accumulation in Cultured Endothelial Cells: The Role of Thioredoxin Interacting Protein
title_full_unstemmed Vitamin D Prevents High Glucose-Induced Lipid Droplets Accumulation in Cultured Endothelial Cells: The Role of Thioredoxin Interacting Protein
title_short Vitamin D Prevents High Glucose-Induced Lipid Droplets Accumulation in Cultured Endothelial Cells: The Role of Thioredoxin Interacting Protein
title_sort vitamin d prevents high glucose-induced lipid droplets accumulation in cultured endothelial cells: the role of thioredoxin interacting protein
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8698366/
https://www.ncbi.nlm.nih.gov/pubmed/34944690
http://dx.doi.org/10.3390/biomedicines9121874
work_keys_str_mv AT scrimieriroberta vitamindpreventshighglucoseinducedlipiddropletsaccumulationinculturedendothelialcellstheroleofthioredoxininteractingprotein
AT cazzanigaalessandra vitamindpreventshighglucoseinducedlipiddropletsaccumulationinculturedendothelialcellstheroleofthioredoxininteractingprotein
AT castiglionisara vitamindpreventshighglucoseinducedlipiddropletsaccumulationinculturedendothelialcellstheroleofthioredoxininteractingprotein
AT maierjeanetteam vitamindpreventshighglucoseinducedlipiddropletsaccumulationinculturedendothelialcellstheroleofthioredoxininteractingprotein