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Differences in Distribution and Biological Effects of F(3)O(4)@PEG Nanoparticles in Normotensive and Hypertensive Rats—Focus on Vascular Function and Liver

We investigate the distribution and biological effects of polyethylene glycol (PEG)-coated magnetite (Fe(3)O(4)@PEG) nanoparticles (~30 nm core size, ~51 nm hydrodynamic size, 2 mg Fe/kg/day, intravenously, for two days) in the aorta and liver of Wistar–Kyoto (WKY) and spontaneously hypertensive rat...

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Autores principales: Micurova, Andrea, Kluknavsky, Michal, Liskova, Silvia, Balis, Peter, Skratek, Martin, Okruhlicova, Ludmila, Manka, Jan, Bernatova, Iveta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8698428/
https://www.ncbi.nlm.nih.gov/pubmed/34944671
http://dx.doi.org/10.3390/biomedicines9121855
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author Micurova, Andrea
Kluknavsky, Michal
Liskova, Silvia
Balis, Peter
Skratek, Martin
Okruhlicova, Ludmila
Manka, Jan
Bernatova, Iveta
author_facet Micurova, Andrea
Kluknavsky, Michal
Liskova, Silvia
Balis, Peter
Skratek, Martin
Okruhlicova, Ludmila
Manka, Jan
Bernatova, Iveta
author_sort Micurova, Andrea
collection PubMed
description We investigate the distribution and biological effects of polyethylene glycol (PEG)-coated magnetite (Fe(3)O(4)@PEG) nanoparticles (~30 nm core size, ~51 nm hydrodynamic size, 2 mg Fe/kg/day, intravenously, for two days) in the aorta and liver of Wistar–Kyoto (WKY) and spontaneously hypertensive rats (SHR). Fe(3)O(4)@PEG had no effect on open-field behaviour but reduced the blood pressure (BP) of Fe(3)O(4)@PEG-treated SHR (SHRu) significantly, compared to both Fe(3)O(4)@PEG-treated WKY (WKYu) and saline-treated control SHR (SHRc). The Fe(3)O(4)@PEG content was significantly elevated in the aorta and liver of SHRu vs. WKYu. Nitric oxide synthase (NOS) activity was unaltered in the aorta, but significantly increased in the liver of SHRu vs. SHRc. In the aorta, Fe(3)O(4)@PEG treatment increased eNOS, iNOS, NRF2, and DMT1 gene expression (considered main effects). In the liver, Fe(3)O(4)@PEG significantly elevated eNOS and iNOS gene expression in SHRu vs. SHRc, as well as DMT1 and FTH1 gene expression (considered main effects). Noradrenaline-induced contractions of the femoral arteries were elevated, while endothelium-dependent contractions were reduced in SHRu vs. SHRc. No differences were found in these parameters in WKY rats. In conclusion, the results indicated that the altered haemodynamics in SHR affect the tissue distribution and selected biological effects of Fe(3)O(4)@PEG in the vasculature and liver, suggesting that caution should be taken when using iron oxide nanoparticles in hypertensive subjects.
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spelling pubmed-86984282021-12-24 Differences in Distribution and Biological Effects of F(3)O(4)@PEG Nanoparticles in Normotensive and Hypertensive Rats—Focus on Vascular Function and Liver Micurova, Andrea Kluknavsky, Michal Liskova, Silvia Balis, Peter Skratek, Martin Okruhlicova, Ludmila Manka, Jan Bernatova, Iveta Biomedicines Article We investigate the distribution and biological effects of polyethylene glycol (PEG)-coated magnetite (Fe(3)O(4)@PEG) nanoparticles (~30 nm core size, ~51 nm hydrodynamic size, 2 mg Fe/kg/day, intravenously, for two days) in the aorta and liver of Wistar–Kyoto (WKY) and spontaneously hypertensive rats (SHR). Fe(3)O(4)@PEG had no effect on open-field behaviour but reduced the blood pressure (BP) of Fe(3)O(4)@PEG-treated SHR (SHRu) significantly, compared to both Fe(3)O(4)@PEG-treated WKY (WKYu) and saline-treated control SHR (SHRc). The Fe(3)O(4)@PEG content was significantly elevated in the aorta and liver of SHRu vs. WKYu. Nitric oxide synthase (NOS) activity was unaltered in the aorta, but significantly increased in the liver of SHRu vs. SHRc. In the aorta, Fe(3)O(4)@PEG treatment increased eNOS, iNOS, NRF2, and DMT1 gene expression (considered main effects). In the liver, Fe(3)O(4)@PEG significantly elevated eNOS and iNOS gene expression in SHRu vs. SHRc, as well as DMT1 and FTH1 gene expression (considered main effects). Noradrenaline-induced contractions of the femoral arteries were elevated, while endothelium-dependent contractions were reduced in SHRu vs. SHRc. No differences were found in these parameters in WKY rats. In conclusion, the results indicated that the altered haemodynamics in SHR affect the tissue distribution and selected biological effects of Fe(3)O(4)@PEG in the vasculature and liver, suggesting that caution should be taken when using iron oxide nanoparticles in hypertensive subjects. MDPI 2021-12-07 /pmc/articles/PMC8698428/ /pubmed/34944671 http://dx.doi.org/10.3390/biomedicines9121855 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Micurova, Andrea
Kluknavsky, Michal
Liskova, Silvia
Balis, Peter
Skratek, Martin
Okruhlicova, Ludmila
Manka, Jan
Bernatova, Iveta
Differences in Distribution and Biological Effects of F(3)O(4)@PEG Nanoparticles in Normotensive and Hypertensive Rats—Focus on Vascular Function and Liver
title Differences in Distribution and Biological Effects of F(3)O(4)@PEG Nanoparticles in Normotensive and Hypertensive Rats—Focus on Vascular Function and Liver
title_full Differences in Distribution and Biological Effects of F(3)O(4)@PEG Nanoparticles in Normotensive and Hypertensive Rats—Focus on Vascular Function and Liver
title_fullStr Differences in Distribution and Biological Effects of F(3)O(4)@PEG Nanoparticles in Normotensive and Hypertensive Rats—Focus on Vascular Function and Liver
title_full_unstemmed Differences in Distribution and Biological Effects of F(3)O(4)@PEG Nanoparticles in Normotensive and Hypertensive Rats—Focus on Vascular Function and Liver
title_short Differences in Distribution and Biological Effects of F(3)O(4)@PEG Nanoparticles in Normotensive and Hypertensive Rats—Focus on Vascular Function and Liver
title_sort differences in distribution and biological effects of f(3)o(4)@peg nanoparticles in normotensive and hypertensive rats—focus on vascular function and liver
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8698428/
https://www.ncbi.nlm.nih.gov/pubmed/34944671
http://dx.doi.org/10.3390/biomedicines9121855
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