Cargando…
tRNA Derivatives in Multiple Myeloma: Investigation of the Potential Value of a tRNA-Derived Molecular Signature
Multiple myeloma (MM) is a hematologic malignancy arising from the clonal proliferation of malignant plasma cells. tRNA-derived RNA fragments (tRFs) constitute a class of small non-coding RNAs, deriving from specific enzymatic cleavage of tRNAs. To the best of our knowledge, this is one of few studi...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8698603/ https://www.ncbi.nlm.nih.gov/pubmed/34944627 http://dx.doi.org/10.3390/biomedicines9121811 |
_version_ | 1784620316651159552 |
---|---|
author | Karousi, Paraskevi Papanota, Aristea-Maria Artemaki, Pinelopi I. Liacos, Christine-Ivy Patseas, Dimitrios Mavrianou-Koutsoukou, Nefeli Liosi, Aikaterini-Anna Kalioraki, Maria-Anna Ntanasis-Stathopoulos, Ioannis Gavriatopoulou, Maria Kastritis, Efstathios Dimopoulos, Meletios-Athanasios Scorilas, Andreas Terpos, Evangelos Kontos, Christos K. |
author_facet | Karousi, Paraskevi Papanota, Aristea-Maria Artemaki, Pinelopi I. Liacos, Christine-Ivy Patseas, Dimitrios Mavrianou-Koutsoukou, Nefeli Liosi, Aikaterini-Anna Kalioraki, Maria-Anna Ntanasis-Stathopoulos, Ioannis Gavriatopoulou, Maria Kastritis, Efstathios Dimopoulos, Meletios-Athanasios Scorilas, Andreas Terpos, Evangelos Kontos, Christos K. |
author_sort | Karousi, Paraskevi |
collection | PubMed |
description | Multiple myeloma (MM) is a hematologic malignancy arising from the clonal proliferation of malignant plasma cells. tRNA-derived RNA fragments (tRFs) constitute a class of small non-coding RNAs, deriving from specific enzymatic cleavage of tRNAs. To the best of our knowledge, this is one of few studies to uncover the potential clinical significance of tRFs in MM. Total RNA was extracted from CD138+ plasma cells of MM and smoldering MM patients, and in vitro polyadenylated. First-strand cDNA synthesis was performed, priming from an oligo-dT-adaptor sequence. Next, real-time quantitative PCR (qPCR) assays were developed for the quantification of six tRFs. Biostatistical analysis was performed to assess the results and in silico analysis was conducted to predict the function of one of the tRFs. Our results showed that elevated levels of five out of six tRFs are indicators of favorable prognosis in MM, predicting prolonged overall survival (OS), while two of them constitute potential molecular biomarkers of favorable prognosis in terms of disease progression. Moreover, three tRFs could be used as surrogate prognostic biomarkers along with the R-ISS staging system to predict OS. In conclusion, tRFs show molecular biomarker utility in MM, while their mechanisms of function merit further investigation. |
format | Online Article Text |
id | pubmed-8698603 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86986032021-12-24 tRNA Derivatives in Multiple Myeloma: Investigation of the Potential Value of a tRNA-Derived Molecular Signature Karousi, Paraskevi Papanota, Aristea-Maria Artemaki, Pinelopi I. Liacos, Christine-Ivy Patseas, Dimitrios Mavrianou-Koutsoukou, Nefeli Liosi, Aikaterini-Anna Kalioraki, Maria-Anna Ntanasis-Stathopoulos, Ioannis Gavriatopoulou, Maria Kastritis, Efstathios Dimopoulos, Meletios-Athanasios Scorilas, Andreas Terpos, Evangelos Kontos, Christos K. Biomedicines Article Multiple myeloma (MM) is a hematologic malignancy arising from the clonal proliferation of malignant plasma cells. tRNA-derived RNA fragments (tRFs) constitute a class of small non-coding RNAs, deriving from specific enzymatic cleavage of tRNAs. To the best of our knowledge, this is one of few studies to uncover the potential clinical significance of tRFs in MM. Total RNA was extracted from CD138+ plasma cells of MM and smoldering MM patients, and in vitro polyadenylated. First-strand cDNA synthesis was performed, priming from an oligo-dT-adaptor sequence. Next, real-time quantitative PCR (qPCR) assays were developed for the quantification of six tRFs. Biostatistical analysis was performed to assess the results and in silico analysis was conducted to predict the function of one of the tRFs. Our results showed that elevated levels of five out of six tRFs are indicators of favorable prognosis in MM, predicting prolonged overall survival (OS), while two of them constitute potential molecular biomarkers of favorable prognosis in terms of disease progression. Moreover, three tRFs could be used as surrogate prognostic biomarkers along with the R-ISS staging system to predict OS. In conclusion, tRFs show molecular biomarker utility in MM, while their mechanisms of function merit further investigation. MDPI 2021-12-01 /pmc/articles/PMC8698603/ /pubmed/34944627 http://dx.doi.org/10.3390/biomedicines9121811 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Karousi, Paraskevi Papanota, Aristea-Maria Artemaki, Pinelopi I. Liacos, Christine-Ivy Patseas, Dimitrios Mavrianou-Koutsoukou, Nefeli Liosi, Aikaterini-Anna Kalioraki, Maria-Anna Ntanasis-Stathopoulos, Ioannis Gavriatopoulou, Maria Kastritis, Efstathios Dimopoulos, Meletios-Athanasios Scorilas, Andreas Terpos, Evangelos Kontos, Christos K. tRNA Derivatives in Multiple Myeloma: Investigation of the Potential Value of a tRNA-Derived Molecular Signature |
title | tRNA Derivatives in Multiple Myeloma: Investigation of the Potential Value of a tRNA-Derived Molecular Signature |
title_full | tRNA Derivatives in Multiple Myeloma: Investigation of the Potential Value of a tRNA-Derived Molecular Signature |
title_fullStr | tRNA Derivatives in Multiple Myeloma: Investigation of the Potential Value of a tRNA-Derived Molecular Signature |
title_full_unstemmed | tRNA Derivatives in Multiple Myeloma: Investigation of the Potential Value of a tRNA-Derived Molecular Signature |
title_short | tRNA Derivatives in Multiple Myeloma: Investigation of the Potential Value of a tRNA-Derived Molecular Signature |
title_sort | trna derivatives in multiple myeloma: investigation of the potential value of a trna-derived molecular signature |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8698603/ https://www.ncbi.nlm.nih.gov/pubmed/34944627 http://dx.doi.org/10.3390/biomedicines9121811 |
work_keys_str_mv | AT karousiparaskevi trnaderivativesinmultiplemyelomainvestigationofthepotentialvalueofatrnaderivedmolecularsignature AT papanotaaristeamaria trnaderivativesinmultiplemyelomainvestigationofthepotentialvalueofatrnaderivedmolecularsignature AT artemakipinelopii trnaderivativesinmultiplemyelomainvestigationofthepotentialvalueofatrnaderivedmolecularsignature AT liacoschristineivy trnaderivativesinmultiplemyelomainvestigationofthepotentialvalueofatrnaderivedmolecularsignature AT patseasdimitrios trnaderivativesinmultiplemyelomainvestigationofthepotentialvalueofatrnaderivedmolecularsignature AT mavrianoukoutsoukounefeli trnaderivativesinmultiplemyelomainvestigationofthepotentialvalueofatrnaderivedmolecularsignature AT liosiaikaterinianna trnaderivativesinmultiplemyelomainvestigationofthepotentialvalueofatrnaderivedmolecularsignature AT kaliorakimariaanna trnaderivativesinmultiplemyelomainvestigationofthepotentialvalueofatrnaderivedmolecularsignature AT ntanasisstathopoulosioannis trnaderivativesinmultiplemyelomainvestigationofthepotentialvalueofatrnaderivedmolecularsignature AT gavriatopouloumaria trnaderivativesinmultiplemyelomainvestigationofthepotentialvalueofatrnaderivedmolecularsignature AT kastritisefstathios trnaderivativesinmultiplemyelomainvestigationofthepotentialvalueofatrnaderivedmolecularsignature AT dimopoulosmeletiosathanasios trnaderivativesinmultiplemyelomainvestigationofthepotentialvalueofatrnaderivedmolecularsignature AT scorilasandreas trnaderivativesinmultiplemyelomainvestigationofthepotentialvalueofatrnaderivedmolecularsignature AT terposevangelos trnaderivativesinmultiplemyelomainvestigationofthepotentialvalueofatrnaderivedmolecularsignature AT kontoschristosk trnaderivativesinmultiplemyelomainvestigationofthepotentialvalueofatrnaderivedmolecularsignature |