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Non-Antibody-Based Binders for the Enrichment of Proteins for Analysis by Mass Spectrometry

There is often a need to isolate proteins from body fluids, such as plasma or serum, prior to further analysis with (targeted) mass spectrometry. Although immunoglobulin or antibody-based binders have been successful in this regard, they possess certain disadvantages, which stimulated the developmen...

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Detalles Bibliográficos
Autores principales: Olaleye, Oladapo, Govorukhina, Natalia, van de Merbel, Nico C., Bischoff, Rainer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8698613/
https://www.ncbi.nlm.nih.gov/pubmed/34944435
http://dx.doi.org/10.3390/biom11121791
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author Olaleye, Oladapo
Govorukhina, Natalia
van de Merbel, Nico C.
Bischoff, Rainer
author_facet Olaleye, Oladapo
Govorukhina, Natalia
van de Merbel, Nico C.
Bischoff, Rainer
author_sort Olaleye, Oladapo
collection PubMed
description There is often a need to isolate proteins from body fluids, such as plasma or serum, prior to further analysis with (targeted) mass spectrometry. Although immunoglobulin or antibody-based binders have been successful in this regard, they possess certain disadvantages, which stimulated the development and validation of alternative, non-antibody-based binders. These binders are based on different protein scaffolds and are often selected and optimized using phage or other display technologies. This review focuses on several non-antibody-based binders in the context of enriching proteins for subsequent liquid chromatography-mass spectrometry (LC-MS) analysis and compares them to antibodies. In addition, we give a brief introduction to approaches for the immobilization of binders. The combination of non-antibody-based binders and targeted mass spectrometry is promising in areas, like regulated bioanalysis of therapeutic proteins or the quantification of biomarkers. However, the rather limited commercial availability of these binders presents a bottleneck that needs to be addressed.
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spelling pubmed-86986132021-12-24 Non-Antibody-Based Binders for the Enrichment of Proteins for Analysis by Mass Spectrometry Olaleye, Oladapo Govorukhina, Natalia van de Merbel, Nico C. Bischoff, Rainer Biomolecules Review There is often a need to isolate proteins from body fluids, such as plasma or serum, prior to further analysis with (targeted) mass spectrometry. Although immunoglobulin or antibody-based binders have been successful in this regard, they possess certain disadvantages, which stimulated the development and validation of alternative, non-antibody-based binders. These binders are based on different protein scaffolds and are often selected and optimized using phage or other display technologies. This review focuses on several non-antibody-based binders in the context of enriching proteins for subsequent liquid chromatography-mass spectrometry (LC-MS) analysis and compares them to antibodies. In addition, we give a brief introduction to approaches for the immobilization of binders. The combination of non-antibody-based binders and targeted mass spectrometry is promising in areas, like regulated bioanalysis of therapeutic proteins or the quantification of biomarkers. However, the rather limited commercial availability of these binders presents a bottleneck that needs to be addressed. MDPI 2021-11-30 /pmc/articles/PMC8698613/ /pubmed/34944435 http://dx.doi.org/10.3390/biom11121791 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Olaleye, Oladapo
Govorukhina, Natalia
van de Merbel, Nico C.
Bischoff, Rainer
Non-Antibody-Based Binders for the Enrichment of Proteins for Analysis by Mass Spectrometry
title Non-Antibody-Based Binders for the Enrichment of Proteins for Analysis by Mass Spectrometry
title_full Non-Antibody-Based Binders for the Enrichment of Proteins for Analysis by Mass Spectrometry
title_fullStr Non-Antibody-Based Binders for the Enrichment of Proteins for Analysis by Mass Spectrometry
title_full_unstemmed Non-Antibody-Based Binders for the Enrichment of Proteins for Analysis by Mass Spectrometry
title_short Non-Antibody-Based Binders for the Enrichment of Proteins for Analysis by Mass Spectrometry
title_sort non-antibody-based binders for the enrichment of proteins for analysis by mass spectrometry
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8698613/
https://www.ncbi.nlm.nih.gov/pubmed/34944435
http://dx.doi.org/10.3390/biom11121791
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