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Non-Antibody-Based Binders for the Enrichment of Proteins for Analysis by Mass Spectrometry
There is often a need to isolate proteins from body fluids, such as plasma or serum, prior to further analysis with (targeted) mass spectrometry. Although immunoglobulin or antibody-based binders have been successful in this regard, they possess certain disadvantages, which stimulated the developmen...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8698613/ https://www.ncbi.nlm.nih.gov/pubmed/34944435 http://dx.doi.org/10.3390/biom11121791 |
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author | Olaleye, Oladapo Govorukhina, Natalia van de Merbel, Nico C. Bischoff, Rainer |
author_facet | Olaleye, Oladapo Govorukhina, Natalia van de Merbel, Nico C. Bischoff, Rainer |
author_sort | Olaleye, Oladapo |
collection | PubMed |
description | There is often a need to isolate proteins from body fluids, such as plasma or serum, prior to further analysis with (targeted) mass spectrometry. Although immunoglobulin or antibody-based binders have been successful in this regard, they possess certain disadvantages, which stimulated the development and validation of alternative, non-antibody-based binders. These binders are based on different protein scaffolds and are often selected and optimized using phage or other display technologies. This review focuses on several non-antibody-based binders in the context of enriching proteins for subsequent liquid chromatography-mass spectrometry (LC-MS) analysis and compares them to antibodies. In addition, we give a brief introduction to approaches for the immobilization of binders. The combination of non-antibody-based binders and targeted mass spectrometry is promising in areas, like regulated bioanalysis of therapeutic proteins or the quantification of biomarkers. However, the rather limited commercial availability of these binders presents a bottleneck that needs to be addressed. |
format | Online Article Text |
id | pubmed-8698613 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86986132021-12-24 Non-Antibody-Based Binders for the Enrichment of Proteins for Analysis by Mass Spectrometry Olaleye, Oladapo Govorukhina, Natalia van de Merbel, Nico C. Bischoff, Rainer Biomolecules Review There is often a need to isolate proteins from body fluids, such as plasma or serum, prior to further analysis with (targeted) mass spectrometry. Although immunoglobulin or antibody-based binders have been successful in this regard, they possess certain disadvantages, which stimulated the development and validation of alternative, non-antibody-based binders. These binders are based on different protein scaffolds and are often selected and optimized using phage or other display technologies. This review focuses on several non-antibody-based binders in the context of enriching proteins for subsequent liquid chromatography-mass spectrometry (LC-MS) analysis and compares them to antibodies. In addition, we give a brief introduction to approaches for the immobilization of binders. The combination of non-antibody-based binders and targeted mass spectrometry is promising in areas, like regulated bioanalysis of therapeutic proteins or the quantification of biomarkers. However, the rather limited commercial availability of these binders presents a bottleneck that needs to be addressed. MDPI 2021-11-30 /pmc/articles/PMC8698613/ /pubmed/34944435 http://dx.doi.org/10.3390/biom11121791 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Olaleye, Oladapo Govorukhina, Natalia van de Merbel, Nico C. Bischoff, Rainer Non-Antibody-Based Binders for the Enrichment of Proteins for Analysis by Mass Spectrometry |
title | Non-Antibody-Based Binders for the Enrichment of Proteins for Analysis by Mass Spectrometry |
title_full | Non-Antibody-Based Binders for the Enrichment of Proteins for Analysis by Mass Spectrometry |
title_fullStr | Non-Antibody-Based Binders for the Enrichment of Proteins for Analysis by Mass Spectrometry |
title_full_unstemmed | Non-Antibody-Based Binders for the Enrichment of Proteins for Analysis by Mass Spectrometry |
title_short | Non-Antibody-Based Binders for the Enrichment of Proteins for Analysis by Mass Spectrometry |
title_sort | non-antibody-based binders for the enrichment of proteins for analysis by mass spectrometry |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8698613/ https://www.ncbi.nlm.nih.gov/pubmed/34944435 http://dx.doi.org/10.3390/biom11121791 |
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