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Culture-Independent Survey of Thermophilic Microbial Communities of the North Caucasus

SIMPLE SUMMARY: The Republic of North Ossetia-Alania, located in the southern part of the North Caucasus, possess a number of hydrothermal habitats, including both subterranean thermal reservoirs and terrestrial hot springs. At the same time, reports on microbiology of numerous geothermal sites are...

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Autores principales: Toshchakov, Stepan V., Izotova, Anna O., Vinogradova, Elizaveta N., Kachmazov, Gennady S., Tuaeva, Albina Y., Abaev, Vladimir T., Evteeva, Martha A., Gunitseva, Natalia M., Korzhenkov, Aleksei A., Elcheninov, Alexander G., Patrushev, Maxim V., Kublanov, Ilya V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8698779/
https://www.ncbi.nlm.nih.gov/pubmed/34943267
http://dx.doi.org/10.3390/biology10121352
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author Toshchakov, Stepan V.
Izotova, Anna O.
Vinogradova, Elizaveta N.
Kachmazov, Gennady S.
Tuaeva, Albina Y.
Abaev, Vladimir T.
Evteeva, Martha A.
Gunitseva, Natalia M.
Korzhenkov, Aleksei A.
Elcheninov, Alexander G.
Patrushev, Maxim V.
Kublanov, Ilya V.
author_facet Toshchakov, Stepan V.
Izotova, Anna O.
Vinogradova, Elizaveta N.
Kachmazov, Gennady S.
Tuaeva, Albina Y.
Abaev, Vladimir T.
Evteeva, Martha A.
Gunitseva, Natalia M.
Korzhenkov, Aleksei A.
Elcheninov, Alexander G.
Patrushev, Maxim V.
Kublanov, Ilya V.
author_sort Toshchakov, Stepan V.
collection PubMed
description SIMPLE SUMMARY: The Republic of North Ossetia-Alania, located in the southern part of the North Caucasus, possess a number of hydrothermal habitats, including both subterranean thermal reservoirs and terrestrial hot springs. At the same time, reports on microbiology of numerous geothermal sites are rather scarce for the whole North Caucasus region. In this paper, we report on the first culture-independent metabarcoding study of thermal habitats in the North Caucasus, coupled with a chemical analysis of the elemental composition of water. The results of this work include the conclusions regarding key metabolic characteristics of these habitats as well as detection of few but abundant deep lineages of uncultivated microorganisms which could be regarded as endemic. This study may represent a first step in closing the knowledge gap in extremophilic microbial communities of the North Caucasus. ABSTRACT: The Greater Caucasus is a part of seismically active Alpine–Himalayan orogenic belt and has been a center of significant volcanic activity during the Quaternary period. That led to the formation of the number of hydrothermal habitats, including subterranean thermal aquifers and surface hot springs. However, there are only a limited number of scientific works reporting on the microbial communities of these habitats. Moreover, all these reports concern only studies of specific microbial taxa, carried out using classical cultivation approaches. In this work, we present first culture-independent study of hydrotherms in the Republic of North Ossetia-Alania, located in the southern part of the North Caucasus. Using 16S metabarcoding, we analyzed the composition of the microbial communities of two subterranean thermal aquifers and terrestrial hot springs of the Karmadon valley. Analysis of correlations between the chemical composition of water and the representation of key taxa allowed us to identify the key factors determining the formation of microbial communities. In addition, we were able to identify a significant number of highly abundant deep phylogenetic lineages. Our study represents a first glance on the thermophilic microbial communities of the North Caucasus and may serve as a basis for further microbiological studies of the extreme habitats of this region.
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spelling pubmed-86987792021-12-24 Culture-Independent Survey of Thermophilic Microbial Communities of the North Caucasus Toshchakov, Stepan V. Izotova, Anna O. Vinogradova, Elizaveta N. Kachmazov, Gennady S. Tuaeva, Albina Y. Abaev, Vladimir T. Evteeva, Martha A. Gunitseva, Natalia M. Korzhenkov, Aleksei A. Elcheninov, Alexander G. Patrushev, Maxim V. Kublanov, Ilya V. Biology (Basel) Article SIMPLE SUMMARY: The Republic of North Ossetia-Alania, located in the southern part of the North Caucasus, possess a number of hydrothermal habitats, including both subterranean thermal reservoirs and terrestrial hot springs. At the same time, reports on microbiology of numerous geothermal sites are rather scarce for the whole North Caucasus region. In this paper, we report on the first culture-independent metabarcoding study of thermal habitats in the North Caucasus, coupled with a chemical analysis of the elemental composition of water. The results of this work include the conclusions regarding key metabolic characteristics of these habitats as well as detection of few but abundant deep lineages of uncultivated microorganisms which could be regarded as endemic. This study may represent a first step in closing the knowledge gap in extremophilic microbial communities of the North Caucasus. ABSTRACT: The Greater Caucasus is a part of seismically active Alpine–Himalayan orogenic belt and has been a center of significant volcanic activity during the Quaternary period. That led to the formation of the number of hydrothermal habitats, including subterranean thermal aquifers and surface hot springs. However, there are only a limited number of scientific works reporting on the microbial communities of these habitats. Moreover, all these reports concern only studies of specific microbial taxa, carried out using classical cultivation approaches. In this work, we present first culture-independent study of hydrotherms in the Republic of North Ossetia-Alania, located in the southern part of the North Caucasus. Using 16S metabarcoding, we analyzed the composition of the microbial communities of two subterranean thermal aquifers and terrestrial hot springs of the Karmadon valley. Analysis of correlations between the chemical composition of water and the representation of key taxa allowed us to identify the key factors determining the formation of microbial communities. In addition, we were able to identify a significant number of highly abundant deep phylogenetic lineages. Our study represents a first glance on the thermophilic microbial communities of the North Caucasus and may serve as a basis for further microbiological studies of the extreme habitats of this region. MDPI 2021-12-20 /pmc/articles/PMC8698779/ /pubmed/34943267 http://dx.doi.org/10.3390/biology10121352 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Toshchakov, Stepan V.
Izotova, Anna O.
Vinogradova, Elizaveta N.
Kachmazov, Gennady S.
Tuaeva, Albina Y.
Abaev, Vladimir T.
Evteeva, Martha A.
Gunitseva, Natalia M.
Korzhenkov, Aleksei A.
Elcheninov, Alexander G.
Patrushev, Maxim V.
Kublanov, Ilya V.
Culture-Independent Survey of Thermophilic Microbial Communities of the North Caucasus
title Culture-Independent Survey of Thermophilic Microbial Communities of the North Caucasus
title_full Culture-Independent Survey of Thermophilic Microbial Communities of the North Caucasus
title_fullStr Culture-Independent Survey of Thermophilic Microbial Communities of the North Caucasus
title_full_unstemmed Culture-Independent Survey of Thermophilic Microbial Communities of the North Caucasus
title_short Culture-Independent Survey of Thermophilic Microbial Communities of the North Caucasus
title_sort culture-independent survey of thermophilic microbial communities of the north caucasus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8698779/
https://www.ncbi.nlm.nih.gov/pubmed/34943267
http://dx.doi.org/10.3390/biology10121352
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