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Mild Coronavirus Disease 2019 (COVID-19) Is Marked by Systemic Oxidative Stress: A Pilot Study

Oxidative stress has been implicated to play a critical role in the pathophysiology of coronavirus disease 2019 (COVID-19) and may therefore be considered as a relevant therapeutic target. Serum free thiols (R-SH, sulfhydryl groups) comprise a robust marker of systemic oxidative stress, since they a...

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Autores principales: van Eijk, Larissa E., Tami, Adriana, Hillebrands, Jan-Luuk, den Dunnen, Wilfred F. A., de Borst, Martin H., van der Voort, Peter H. J., Bulthuis, Marian L. C., Veloo, Alida C. M., Wold, Karin I., Vincenti González, María F., van der Gun, Bernardina T. F., van Goor, Harry, Bourgonje, Arno R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8698810/
https://www.ncbi.nlm.nih.gov/pubmed/34943125
http://dx.doi.org/10.3390/antiox10122022
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author van Eijk, Larissa E.
Tami, Adriana
Hillebrands, Jan-Luuk
den Dunnen, Wilfred F. A.
de Borst, Martin H.
van der Voort, Peter H. J.
Bulthuis, Marian L. C.
Veloo, Alida C. M.
Wold, Karin I.
Vincenti González, María F.
van der Gun, Bernardina T. F.
van Goor, Harry
Bourgonje, Arno R.
author_facet van Eijk, Larissa E.
Tami, Adriana
Hillebrands, Jan-Luuk
den Dunnen, Wilfred F. A.
de Borst, Martin H.
van der Voort, Peter H. J.
Bulthuis, Marian L. C.
Veloo, Alida C. M.
Wold, Karin I.
Vincenti González, María F.
van der Gun, Bernardina T. F.
van Goor, Harry
Bourgonje, Arno R.
author_sort van Eijk, Larissa E.
collection PubMed
description Oxidative stress has been implicated to play a critical role in the pathophysiology of coronavirus disease 2019 (COVID-19) and may therefore be considered as a relevant therapeutic target. Serum free thiols (R-SH, sulfhydryl groups) comprise a robust marker of systemic oxidative stress, since they are readily oxidized by reactive oxygen species (ROS). In this study, serum free thiol concentrations were measured in hospitalized and non-hospitalized patients with COVID-19 and healthy controls and their associations with relevant clinical parameters were examined. Serum free thiol concentrations were measured colorimetrically (Ellman’s method) in 29 non-hospitalized COVID-19 subjects and 30 age-, sex-, and body-mass index (BMI)-matched healthy controls and analyzed for associations with clinical and biochemical disease parameters. Additional free thiol measurements were performed on seven serum samples from COVID-19 subjects who required hospitalization to examine their correlation with disease severity. Non-hospitalized subjects with COVID-19 had significantly lower concentrations of serum free thiols compared to healthy controls (p = 0.014), indicating oxidative stress. Serum free thiols were positively associated with albumin (St. β = 0.710, p < 0.001) and inversely associated with CRP (St. β = −0.434, p = 0.027), and showed significant discriminative ability to differentiate subjects with COVID-19 from healthy controls (AUC = 0.69, p = 0.011), which was slightly higher than the discriminative performance of CRP concentrations regarding COVID-19 diagnosis (AUC = 0.66, p = 0.042). This study concludes that systemic oxidative stress is increased in patients with COVID-19 compared with healthy controls. This opens an avenue of treatment options since free thiols are amenable to therapeutic modulation.
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spelling pubmed-86988102021-12-24 Mild Coronavirus Disease 2019 (COVID-19) Is Marked by Systemic Oxidative Stress: A Pilot Study van Eijk, Larissa E. Tami, Adriana Hillebrands, Jan-Luuk den Dunnen, Wilfred F. A. de Borst, Martin H. van der Voort, Peter H. J. Bulthuis, Marian L. C. Veloo, Alida C. M. Wold, Karin I. Vincenti González, María F. van der Gun, Bernardina T. F. van Goor, Harry Bourgonje, Arno R. Antioxidants (Basel) Article Oxidative stress has been implicated to play a critical role in the pathophysiology of coronavirus disease 2019 (COVID-19) and may therefore be considered as a relevant therapeutic target. Serum free thiols (R-SH, sulfhydryl groups) comprise a robust marker of systemic oxidative stress, since they are readily oxidized by reactive oxygen species (ROS). In this study, serum free thiol concentrations were measured in hospitalized and non-hospitalized patients with COVID-19 and healthy controls and their associations with relevant clinical parameters were examined. Serum free thiol concentrations were measured colorimetrically (Ellman’s method) in 29 non-hospitalized COVID-19 subjects and 30 age-, sex-, and body-mass index (BMI)-matched healthy controls and analyzed for associations with clinical and biochemical disease parameters. Additional free thiol measurements were performed on seven serum samples from COVID-19 subjects who required hospitalization to examine their correlation with disease severity. Non-hospitalized subjects with COVID-19 had significantly lower concentrations of serum free thiols compared to healthy controls (p = 0.014), indicating oxidative stress. Serum free thiols were positively associated with albumin (St. β = 0.710, p < 0.001) and inversely associated with CRP (St. β = −0.434, p = 0.027), and showed significant discriminative ability to differentiate subjects with COVID-19 from healthy controls (AUC = 0.69, p = 0.011), which was slightly higher than the discriminative performance of CRP concentrations regarding COVID-19 diagnosis (AUC = 0.66, p = 0.042). This study concludes that systemic oxidative stress is increased in patients with COVID-19 compared with healthy controls. This opens an avenue of treatment options since free thiols are amenable to therapeutic modulation. MDPI 2021-12-20 /pmc/articles/PMC8698810/ /pubmed/34943125 http://dx.doi.org/10.3390/antiox10122022 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
van Eijk, Larissa E.
Tami, Adriana
Hillebrands, Jan-Luuk
den Dunnen, Wilfred F. A.
de Borst, Martin H.
van der Voort, Peter H. J.
Bulthuis, Marian L. C.
Veloo, Alida C. M.
Wold, Karin I.
Vincenti González, María F.
van der Gun, Bernardina T. F.
van Goor, Harry
Bourgonje, Arno R.
Mild Coronavirus Disease 2019 (COVID-19) Is Marked by Systemic Oxidative Stress: A Pilot Study
title Mild Coronavirus Disease 2019 (COVID-19) Is Marked by Systemic Oxidative Stress: A Pilot Study
title_full Mild Coronavirus Disease 2019 (COVID-19) Is Marked by Systemic Oxidative Stress: A Pilot Study
title_fullStr Mild Coronavirus Disease 2019 (COVID-19) Is Marked by Systemic Oxidative Stress: A Pilot Study
title_full_unstemmed Mild Coronavirus Disease 2019 (COVID-19) Is Marked by Systemic Oxidative Stress: A Pilot Study
title_short Mild Coronavirus Disease 2019 (COVID-19) Is Marked by Systemic Oxidative Stress: A Pilot Study
title_sort mild coronavirus disease 2019 (covid-19) is marked by systemic oxidative stress: a pilot study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8698810/
https://www.ncbi.nlm.nih.gov/pubmed/34943125
http://dx.doi.org/10.3390/antiox10122022
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