Soil Fungal Diversity of the Aguarongo Andean Forest (Ecuador)

SIMPLE SUMMARY: The Kingdom Fungi is one of the richest in species, most of which are still unknown. Many fungal species are hidden in the tropics, the area richest in biodiversity on earth. In this paper, a mycological analysis is presented on a vast number of soil samples collected in the Aguarongo forest, an important Andean Natural Reserve of Ecuador. The study was carried out by analyzing the total DNA extracted from the soil and unveiled a total of more than 400 species of fungi. The most abundant species belong to Ascomycota and Mortierellomycota; some are important beneficial fungi for the environments such as antagonistics of fungal pathogens or nematode predators, while others are well-known producers of nutraceutical and pharmaceutical compounds. Based on the results of this study, a picture of the mycodiversity of Aguarongo forest soil was obtained. This area hides a huge number of unknown fungal species that could be discovered; thus, the protection of the Aguarongo forest is mandatory. ABSTRACT: Fungi represent an essential component of ecosystems, functioning as decomposers and biotrophs, and they are one of the most diverse groups of Eukarya. In the tropics, many species are unknown. In this work, high-throughput DNA sequencing was used to discover the biodiversity of soil fungi in the Aguarongo forest reserve, one of the richest biodiversity hotspots in Ecuador. The rDNA metabarcoding analysis revealed the presence of seven phyla: Ascomycota, Basidiomycota, Mortierellomycota, Mucoromycota, Glomeromycota, Chytridiomycota, and Monoblepharomycota. A total of 440 identified species were recorded. They mainly belonged to Ascomycota (263) and Basidiomycota (127). In Mortierellomycota, 12 species were recorded, among which Podila verticillata is extremely frequent and represents the dominant species in the entire mycobiota of Aguarongo. The present research provides the first account of the entire soil mycobiota in the Aguarongo forest, where many fungal species exist that have strong application potential in agriculture, bioremediation, chemical, and the food industry. The Aguarongo forest hides a huge number of unknown fungal species that could be assessed, and its protection is of the utmost importance.