Fitness Analysis and Transcriptome Profiling Following Repeated Mild Heat Stress of Varying Frequency in Drosophila melanogaster Females

SIMPLE SUMMARY: We studied the effect of mild heat stress (38 °C, 1 h) occurring once a day or once a week on D. melanogaster fertility, longevity, body composition metabolism and differential gene expression in fat body and adjacent tissues. Weekly stress in the first two weeks did not affect longevity but caused a decrease in fat content and an increase in the total level of fertility. Daily stress caused a significant longevity, fertility and fat content decrease, but an increase in carbohydrate levels compared with the control group. These data agree well with the results of transcriptome analysis, which demonstrated significant changes in expression levels of genes involved in proteolysis/digestion following daily stress. Heat shock protein 23 and stress-inducible humoral factor Turandot gene network are also involved. It is notable that daily and weekly heat stress resulted in different changes in metabolism, fitness and differential gene expression. ABSTRACT: Understanding how repeated stress affects metabolic and physiological functions in the long run is of crucial importance for evaluating anthropogenic pressure on the environment. We investigated fertility, longevity and metabolism in D. melanogaster females exposed to short-term heat stress (38 °C, 1 h) repeated daily or weekly. Daily stress was shown to cause a significant decrease in both fertility and longevity, as well as in body mass and triglyceride (fat) content, but a significant increase in trehalose and glucose content. Weekly stress did not affect longevity and carbohydrate metabolism but resulted in a significant decrease in body mass and fat content. Weekly stress did not affect the total level of fertility, despite sharp fertility drops on the exact days of stressing. However, stressing insects weekly, only in the first two weeks after eclosion, caused a significant increase in the total level of fertility. The analysis of differentially expressed genes in the fat bodies and adjacent tissues of researched groups with the use of RNA-Seq profiling revealed changes in signal pathways related to proteolysis/digestion, heat shock protein 23, and in the tightly linked stress-inducible humoral factor Turandot gene network.