Prevalence and Molecular Diversity of Plant-Parasitic Nematodes of Yam (Dioscorea spp.) in China, with Focus on Merlinius spp.

SIMPLE SUMMARY: Plant nematodes (PPNs) have been documented as economically important pests of yam in different parts of the world with Pratylenchus spp. and Meloidogyne spp. being the most widespread and destructive pests in Asia, causing significant yield losses. The primary inspiration for this study is the scarcity of information about nematode pests of yam in China and the fact that no previous study has looked into the prevalence, diversity, and integrative taxonomy of PPNs species in China cropping systems, as well as their consequences on yam cultivation. Therefore, the objectives of this study were to conduct a nematode study in south-eastern China to (i) record the prevalence and diversity of PPNs of cultivated yam with emphasis on the five prominent genera identified, and (ii) characterized morphologically, morphometrically, and molecularly Merlinius spp. The high prevalence of the Merlinius spp. revealed that the species may be more important to yam than previously thought. Conclusively, this study provided useful baseline PPNs data for yam in China. Hence, future study should focus on; developing innovative, environmentally friendly, and cost-effective nematode management strategies to control this pest that damage farmers’ yam fields in China, and constantly updating information of PPNs species found to allow proactive measures in terms of their spread and management. ABSTRACT: There is little information about nematode pests associated with yam in China. Between 2020 and 2021, surveys of yam fields were conducted to investigate the abundance and prevalence of plant-parasitic nematodes in major yam growing areas. A total of 110 bulk soil samples from the yam rhizosphere and 48 yam tubers were collected from seven counties in Jiangxi and Shandong provinces. Standard protocols were used to extract nematodes from soil and tubers and identified at the genus level. In this study, 16 species and 13 nematode genera were recorded. The five most prominent species on the yam rhizosphere according to mean population densities were Pratylenchus coffeae (291/individuals), Meloidogyne (262/individuals), Rotylenchulus reniformis (225/individuals), Merlinius (224/individuals), and Helicotylenchus dihystera (171/individuals). In the tubers, the three most prominent species were Pratylenchus coffeae (415/individuals), Meloidogyne (331/individuals), and Rotylenchulus reniformis (115/individuals). These species were verified with appropriate molecular analysis. The high prevalence of the ectoparasite (Merlinius spp.) on the rhizosphere of yam also revealed that Merlinius spp. May be more important to yam than previously thought. Morphological and molecular analyses further confirmed the identity of the species as Merlinius brevidens and were characterized for the first time on yam in China. Minor morphometrical differences (slightly longer body and stylet) were observed in Chinese populations of M. brevidens compared to the original description. Additionally, this study reveals that M. brevidens isolated from China showed a higher nucleotide sequence in the ITS region compared to M. brevidens populations from India. This finding provides baseline information on the nematode pest occurrence on yam in China and calls for effective management.