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SIRT1 Contributes as an Invasiveness Marker in Pituitary Adenoma

The aim of the study was to find the association between SIRT1 concentration, SIRT1 rs3758391, rs3818292, rs7895833 polymorphisms and clinical manifestations of pituitary adenoma (PA). The study included 108 patients with PA and 216 healthy individuals. Using commercial kits, DNA was extracted from...

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Autores principales: Vaiciulis, Domantas, Vilkeviciute, Alvita, Gedvilaite, Greta, Glebauskiene, Brigita, Kriauciuniene, Loresa, Liutkeviciene, Rasa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8699307/
https://www.ncbi.nlm.nih.gov/pubmed/34942940
http://dx.doi.org/10.3390/brainsci11121638
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author Vaiciulis, Domantas
Vilkeviciute, Alvita
Gedvilaite, Greta
Glebauskiene, Brigita
Kriauciuniene, Loresa
Liutkeviciene, Rasa
author_facet Vaiciulis, Domantas
Vilkeviciute, Alvita
Gedvilaite, Greta
Glebauskiene, Brigita
Kriauciuniene, Loresa
Liutkeviciene, Rasa
author_sort Vaiciulis, Domantas
collection PubMed
description The aim of the study was to find the association between SIRT1 concentration, SIRT1 rs3758391, rs3818292, rs7895833 polymorphisms and clinical manifestations of pituitary adenoma (PA). The study included 108 patients with PA and 216 healthy individuals. Using commercial kits, DNA was extracted from peripheral blood leukocytes. To determine the PA and control group subjects genotypes was used real-time PCR method, for SIRT concentration measurement we used ELISA method. The statistical data analysis was completed using the “BM SPSS Statistics 20.0” software. Results: We performed statistical analysis of SNPs in the patient and healthy controls and patients’ subgroups and found statistically significant differences in rs7895833 genotype (A/A, A/G, G/G) distributions between the active PA and control groups (67.9%, 24.6%, 5.7% vs. 72.2%, 27.3%, 0.5%; p = 0.02) Also, the results showed that the rs7895833 G/G genotype is associated with about 13-fold increased odds of active PA development compared to the A/A (OR = 13.95% CI: 1.314–128.632; p = 0.028) and both A/A and A/G genotypes (OR = 12.9; 95% CI: 1.314–126.624; p = 0.028). There is ample evidence that SIRT1 in the pituitary and other target organs modifies the synthesis, secretion, and activity of hormones to trigger adaptive responses, thus we decided to include this in our study. When determining the serum concentration of SIRT1, we did not find a statistically significant difference between the PA group and the control group. SIRT1 serum level was statistically significantly higher in women with PA than in healthy control women (1.115 (3.748) vs. 136 (0.211); p = 0.008). To conclude—SIRT1 rs7895833 G/G genotype is associated with about 13-fold increased odds of active PA development compared to the A/A and both A/A and A/G genotypes. SIRT1 serum levels are higher in women with PA than in healthy women.
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spelling pubmed-86993072021-12-24 SIRT1 Contributes as an Invasiveness Marker in Pituitary Adenoma Vaiciulis, Domantas Vilkeviciute, Alvita Gedvilaite, Greta Glebauskiene, Brigita Kriauciuniene, Loresa Liutkeviciene, Rasa Brain Sci Article The aim of the study was to find the association between SIRT1 concentration, SIRT1 rs3758391, rs3818292, rs7895833 polymorphisms and clinical manifestations of pituitary adenoma (PA). The study included 108 patients with PA and 216 healthy individuals. Using commercial kits, DNA was extracted from peripheral blood leukocytes. To determine the PA and control group subjects genotypes was used real-time PCR method, for SIRT concentration measurement we used ELISA method. The statistical data analysis was completed using the “BM SPSS Statistics 20.0” software. Results: We performed statistical analysis of SNPs in the patient and healthy controls and patients’ subgroups and found statistically significant differences in rs7895833 genotype (A/A, A/G, G/G) distributions between the active PA and control groups (67.9%, 24.6%, 5.7% vs. 72.2%, 27.3%, 0.5%; p = 0.02) Also, the results showed that the rs7895833 G/G genotype is associated with about 13-fold increased odds of active PA development compared to the A/A (OR = 13.95% CI: 1.314–128.632; p = 0.028) and both A/A and A/G genotypes (OR = 12.9; 95% CI: 1.314–126.624; p = 0.028). There is ample evidence that SIRT1 in the pituitary and other target organs modifies the synthesis, secretion, and activity of hormones to trigger adaptive responses, thus we decided to include this in our study. When determining the serum concentration of SIRT1, we did not find a statistically significant difference between the PA group and the control group. SIRT1 serum level was statistically significantly higher in women with PA than in healthy control women (1.115 (3.748) vs. 136 (0.211); p = 0.008). To conclude—SIRT1 rs7895833 G/G genotype is associated with about 13-fold increased odds of active PA development compared to the A/A and both A/A and A/G genotypes. SIRT1 serum levels are higher in women with PA than in healthy women. MDPI 2021-12-11 /pmc/articles/PMC8699307/ /pubmed/34942940 http://dx.doi.org/10.3390/brainsci11121638 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vaiciulis, Domantas
Vilkeviciute, Alvita
Gedvilaite, Greta
Glebauskiene, Brigita
Kriauciuniene, Loresa
Liutkeviciene, Rasa
SIRT1 Contributes as an Invasiveness Marker in Pituitary Adenoma
title SIRT1 Contributes as an Invasiveness Marker in Pituitary Adenoma
title_full SIRT1 Contributes as an Invasiveness Marker in Pituitary Adenoma
title_fullStr SIRT1 Contributes as an Invasiveness Marker in Pituitary Adenoma
title_full_unstemmed SIRT1 Contributes as an Invasiveness Marker in Pituitary Adenoma
title_short SIRT1 Contributes as an Invasiveness Marker in Pituitary Adenoma
title_sort sirt1 contributes as an invasiveness marker in pituitary adenoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8699307/
https://www.ncbi.nlm.nih.gov/pubmed/34942940
http://dx.doi.org/10.3390/brainsci11121638
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