Cargando…

How to Perform a Microfluidic Cultivation Experiment—A Guideline to Success

As a result of the steadily ongoing development of microfluidic cultivation (MC) devices, a plethora of setups is used in biological laboratories for the cultivation and analysis of different organisms. Because of their biocompatibility and ease of fabrication, polydimethylsiloxane (PDMS)-glass-base...

Descripción completa

Detalles Bibliográficos
Autores principales:	Täuber, Sarah, Schmitz, Julian, Blöbaum, Luisa, Fante, Niklas, Steinhoff, Heiko, Grünberger, Alexander
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8699335/ https://www.ncbi.nlm.nih.gov/pubmed/34940242 http://dx.doi.org/10.3390/bios11120485

Ejemplares similares

Protocol to perform dynamic microfluidic single-cell cultivation of C. glutamicum
por: Blöbaum, Luisa, et al.
Publicado: (2023)

Reliable cell retention of mammalian suspension cells in microfluidic cultivation chambers
por: Schmitz, Julian, et al.
Publicado: (2023)

Growth Response and Recovery of Corynebacterium glutamicum Colonies on Single-Cell Level Upon Defined pH Stress Pulses
por: Täuber, Sarah, et al.
Publicado: (2021)

A microfluidic photobioreactor for simultaneous observation and cultivation of single microalgal cells or cell aggregates
por: Westerwalbesloh, Christoph, et al.
Publicado: (2019)

Comparative Single-Cell Analysis of Different E. coli Expression Systems during Microfluidic Cultivation
por: Binder, Dennis, et al.
Publicado: (2016)

Growth and eGFP Production of CHO-K1 Suspension Cells Cultivated From Single Cell to Laboratory Scale
por: Schmitz, Julian, et al.
Publicado: (2021)

Human Blood Serum Induces p38-MAPK- and Hsp27-Dependent Migration Dynamics of Adult Human Cardiac Stem Cells: Single-Cell Analysis via a Microfluidic-Based Cultivation Platform
por: Höving, Anna L., et al.
Publicado: (2021)

Microfluidics for Biotechnology: Bridging Gaps to Foster Microfluidic Applications
por: Ortseifen, Vera, et al.
Publicado: (2020)

In Vitro Cultivation of the Syphilis Spirochete Treponema pallidum
por: Edmondson, Diane G., et al.
Publicado: (2021)

Protocol to assess performance of crisis standards of care guidelines for clinical triage
por: Merriam, Louis T., et al.
Publicado: (2021)

Assessing the growth kinetics and stoichiometry of Escherichia coli at the single‐cell level
por: Smaluch, Katharina, et al.
Publicado: (2022)

Understanding success and failure in multimorbidity: protocol for using realist synthesis to identify how social learning and workplace practices can be optimised
por: Yardley, Sarah, et al.
Publicado: (2013)

Isolation and in vitro cultivation of adrenal cells from mice
por: Friedrich, Linda, et al.
Publicado: (2021)

Protocol for bioelectrochemical enrichment, cultivation, and characterization of extreme electroactive microorganisms
por: Singh, Ramandeep, et al.
Publicado: (2022)

Two Methods for the Isolation and Cultivation of Porcine Primary Corneal Cells
por: Netto, Alice Rocha Teixeira, et al.
Publicado: (2023)

Microfluidic system for in vitro epithelial folding and calcium waves induction
por: Brun-Cosme-Bruny, Marvin, et al.
Publicado: (2022)

How are ontologies implemented to represent clinical practice guidelines in clinical decision support systems: protocol for a systematic review
por: Sadeghi-Ghyassi, Fatemeh, et al.
Publicado: (2022)

Critical parameters and procedures for anaerobic cultivation of yeasts in bioreactors and anaerobic chambers
por: Mooiman, Christiaan, et al.
Publicado: (2021)

Microfluidic platform for spatially segregated experimental evolution studies with E. coli
por: Seo, Seokju, et al.
Publicado: (2022)

Protocol of living cell separation using the microfluidic dielectrophoresis integrated chip
por: Koba, Kazumi, et al.
Publicado: (2022)

Microfluidic encapsulation of Xenopus laevis cell-free extracts using hydrogel photolithography
por: Geisterfer, Zachary M., et al.
Publicado: (2020)

Microfluidics and fluorescence microscopy protocol to study the response of C. elegans to chemosensory stimuli
por: Bruggeman, Christine W., et al.
Publicado: (2023)

Isolation, cultivation and immunostaining of single myofibers: An improved approach to study the behavior of satellite cells
por: Lim, Chai Ling, et al.
Publicado: (2018)

Establishment of in vitro 3D spheroid cell cultivation from human gynecologic cancer tissues
por: Ueda, Haruka, et al.
Publicado: (2021)

How to integrate individual patient values and preferences in clinical practice guidelines? A research protocol
por: van der Weijden, Trudy, et al.
Publicado: (2010)

Reproduction of Large-Scale Bioreactor Conditions on Microfluidic Chips
por: Ho, Phuong, et al.
Publicado: (2019)

Protocol for a systematic review of N-of-1 trial protocol guidelines and protocol reporting guidelines
por: Porcino, Antony J., et al.
Publicado: (2017)

Fabrication of a microfluidic device for studying the combinatorial effect of physical and chemical cues on cell migration
por: Saxena, Neha, et al.
Publicado: (2021)

Microfluidics for single-cell lineage tracking over time to characterize transmission of phenotypes in Saccharomyces cerevisiae
por: Bheda, Poonam, et al.
Publicado: (2020)

Fabricating self-powered microfluidic devices via 3D printing for manipulating fluid flow
por: Woo, Sung Oh, et al.
Publicado: (2022)

Characterizing circulating tumor cells using affinity-based microfluidic capture and AFM-based biomechanics
por: Deliorman, Muhammedin, et al.
Publicado: (2022)

Dissociation and inDrops microfluidic encapsulation of human gut tissues for single-cell atlasing studies
por: Simmons, Alan J., et al.
Publicado: (2022)

Protocol to determine antibody affinity and concentration in complex solutions using microfluidic antibody affinity profiling
por: Emmenegger, Marc, et al.
Publicado: (2023)

A Micropillar Array Based Microfluidic Device for Rare Cell Detection and Single-Cell Proteomics
por: Chen, Kangfu, et al.
Publicado: (2023)

Microfluidic separation of axonal and somal compartments of neural progenitor cells differentiated in a 3D matrix
por: Lotlikar, Madhura S., et al.
Publicado: (2022)

Droplet microfluidic-based loop-mediated isothermal amplification (dLAMP) for simultaneous quantification of multiple targets
por: Tan, Ya-Ling, et al.
Publicado: (2022)

The Use of Frozen, Food-Grade Blood to Successfully Maintain Colonies of Four Species of Mosquitoes (Diptera: Culicidae)
por: Tyler-Julian, Kara, et al.
Publicado: (2021)

How do the experiences and beliefs of adults and children with heterozygous familial hypercholesterolaemia influence their adherence to treatment? A systematic review of qualitative evidence protocol
por: Kinnear, Fiona J., et al.
Publicado: (2018)

Isolation, cultivation, and application of primary respiratory epithelial cells obtained by nasal brushing, polyp samples, or lung explants
por: Golec, Anita, et al.
Publicado: (2022)

How to Cultivate the Sense of Smell
Publicado: (1888)

Cannot write session to /tmp/vufind_sessions/sess_o7t1oslpp7gbhne479asmil6cd