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Targeting CAMKK2 and SOC Channels as a Novel Therapeutic Approach for Sensitizing Acute Promyelocytic Leukemia Cells to All-Trans Retinoic Acid

Calcium ions (Ca(2+)) play important and diverse roles in the regulation of autophagy, cell death and differentiation. Here, we investigated the impact of Ca(2+) in regulating acute promyelocytic leukemia (APL) cell fate in response to the anti-cancer agent all-trans retinoic acid (ATRA). We observe...

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Autores principales: Merhi, Faten, Alvarez-Valadez, Karla, Trepiana, Jenifer, Lescoat, Claire, Groppi, Alexis, Dupuy, Jean-William, Soubeyran, Pierre, Kroemer, Guido, Vacher, Pierre, Djavaheri-Mergny, Mojgan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8699360/
https://www.ncbi.nlm.nih.gov/pubmed/34943872
http://dx.doi.org/10.3390/cells10123364
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author Merhi, Faten
Alvarez-Valadez, Karla
Trepiana, Jenifer
Lescoat, Claire
Groppi, Alexis
Dupuy, Jean-William
Soubeyran, Pierre
Kroemer, Guido
Vacher, Pierre
Djavaheri-Mergny, Mojgan
author_facet Merhi, Faten
Alvarez-Valadez, Karla
Trepiana, Jenifer
Lescoat, Claire
Groppi, Alexis
Dupuy, Jean-William
Soubeyran, Pierre
Kroemer, Guido
Vacher, Pierre
Djavaheri-Mergny, Mojgan
author_sort Merhi, Faten
collection PubMed
description Calcium ions (Ca(2+)) play important and diverse roles in the regulation of autophagy, cell death and differentiation. Here, we investigated the impact of Ca(2+) in regulating acute promyelocytic leukemia (APL) cell fate in response to the anti-cancer agent all-trans retinoic acid (ATRA). We observed that ATRA promotes calcium entry through store-operated calcium (SOC) channels into acute promyelocytic leukemia (APL) cells. This response is associated with changes in the expression profiles of ORAI1 and STIM1, two proteins involved in SOC channels activation, as well as with a significant upregulation of several key proteins associated to calcium signaling. Moreover, ATRA treatment of APL cells led to a significant activation of calcium/calmodulin-dependent protein kinase kinase 2 (CAMKK2) and its downstream effector AMP-activated protein kinase (AMPK), linking Ca(2+) signaling to autophagy. Pharmacological inhibition of SOC channels and CAMKK2 enhanced ATRA-induced cell differentiation and death. Altogether, our results unravel an ATRA-elicited signaling pathway that involves SOC channels/CAMKK2 activation, induction of autophagy, inhibition of cellular differentiation and suppression of cell death. We suggest that SOC channels and CAMKK2 may constitute novel drug targets for potentiating the anti-cancer effect of ATRA in APL patients.
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spelling pubmed-86993602021-12-24 Targeting CAMKK2 and SOC Channels as a Novel Therapeutic Approach for Sensitizing Acute Promyelocytic Leukemia Cells to All-Trans Retinoic Acid Merhi, Faten Alvarez-Valadez, Karla Trepiana, Jenifer Lescoat, Claire Groppi, Alexis Dupuy, Jean-William Soubeyran, Pierre Kroemer, Guido Vacher, Pierre Djavaheri-Mergny, Mojgan Cells Article Calcium ions (Ca(2+)) play important and diverse roles in the regulation of autophagy, cell death and differentiation. Here, we investigated the impact of Ca(2+) in regulating acute promyelocytic leukemia (APL) cell fate in response to the anti-cancer agent all-trans retinoic acid (ATRA). We observed that ATRA promotes calcium entry through store-operated calcium (SOC) channels into acute promyelocytic leukemia (APL) cells. This response is associated with changes in the expression profiles of ORAI1 and STIM1, two proteins involved in SOC channels activation, as well as with a significant upregulation of several key proteins associated to calcium signaling. Moreover, ATRA treatment of APL cells led to a significant activation of calcium/calmodulin-dependent protein kinase kinase 2 (CAMKK2) and its downstream effector AMP-activated protein kinase (AMPK), linking Ca(2+) signaling to autophagy. Pharmacological inhibition of SOC channels and CAMKK2 enhanced ATRA-induced cell differentiation and death. Altogether, our results unravel an ATRA-elicited signaling pathway that involves SOC channels/CAMKK2 activation, induction of autophagy, inhibition of cellular differentiation and suppression of cell death. We suggest that SOC channels and CAMKK2 may constitute novel drug targets for potentiating the anti-cancer effect of ATRA in APL patients. MDPI 2021-11-30 /pmc/articles/PMC8699360/ /pubmed/34943872 http://dx.doi.org/10.3390/cells10123364 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Merhi, Faten
Alvarez-Valadez, Karla
Trepiana, Jenifer
Lescoat, Claire
Groppi, Alexis
Dupuy, Jean-William
Soubeyran, Pierre
Kroemer, Guido
Vacher, Pierre
Djavaheri-Mergny, Mojgan
Targeting CAMKK2 and SOC Channels as a Novel Therapeutic Approach for Sensitizing Acute Promyelocytic Leukemia Cells to All-Trans Retinoic Acid
title Targeting CAMKK2 and SOC Channels as a Novel Therapeutic Approach for Sensitizing Acute Promyelocytic Leukemia Cells to All-Trans Retinoic Acid
title_full Targeting CAMKK2 and SOC Channels as a Novel Therapeutic Approach for Sensitizing Acute Promyelocytic Leukemia Cells to All-Trans Retinoic Acid
title_fullStr Targeting CAMKK2 and SOC Channels as a Novel Therapeutic Approach for Sensitizing Acute Promyelocytic Leukemia Cells to All-Trans Retinoic Acid
title_full_unstemmed Targeting CAMKK2 and SOC Channels as a Novel Therapeutic Approach for Sensitizing Acute Promyelocytic Leukemia Cells to All-Trans Retinoic Acid
title_short Targeting CAMKK2 and SOC Channels as a Novel Therapeutic Approach for Sensitizing Acute Promyelocytic Leukemia Cells to All-Trans Retinoic Acid
title_sort targeting camkk2 and soc channels as a novel therapeutic approach for sensitizing acute promyelocytic leukemia cells to all-trans retinoic acid
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8699360/
https://www.ncbi.nlm.nih.gov/pubmed/34943872
http://dx.doi.org/10.3390/cells10123364
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