Chimeric crRNA improves CRISPR–Cas12a specificity in the N501Y mutation detection of Alpha, Beta, Gamma, and Mu variants of SARS-CoV-2

Many CRISPR/Cas platforms have been established for the detection of SARS-CoV-2. But the detection platform of the variants of SARS-CoV-2 is scarce because its specificity is very challenging to achieve for those with only one or a few nucleotide(s) differences. Here, we report for the first time th...

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Autores principales: Yang, Jun, Barua, Nilakshi, Rahman, Md. Nannur, Lo, Norman, Tsang, Tsz Fung, Yang, Xiao, Chan, Paul K. S., Zhang, Li, Ip, Margaret
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8700041/
https://www.ncbi.nlm.nih.gov/pubmed/34941928
http://dx.doi.org/10.1371/journal.pone.0261778
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author Yang, Jun
Barua, Nilakshi
Rahman, Md. Nannur
Lo, Norman
Tsang, Tsz Fung
Yang, Xiao
Chan, Paul K. S.
Zhang, Li
Ip, Margaret
author_facet Yang, Jun
Barua, Nilakshi
Rahman, Md. Nannur
Lo, Norman
Tsang, Tsz Fung
Yang, Xiao
Chan, Paul K. S.
Zhang, Li
Ip, Margaret
author_sort Yang, Jun
collection PubMed
description Many CRISPR/Cas platforms have been established for the detection of SARS-CoV-2. But the detection platform of the variants of SARS-CoV-2 is scarce because its specificity is very challenging to achieve for those with only one or a few nucleotide(s) differences. Here, we report for the first time that chimeric crRNA could be critical in enhancing the specificity of CRISPR-Cas12a detecting of N501Y, which is shared by Alpha, Beta, Gamma, and Mu variants of SARS-CoV-2 without compromising its sensitivity. This strategy could also be applied to detect other SARS-CoV-2 variants that differ only one or a few nucleotide(s) differences.
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spelling pubmed-87000412021-12-24 Chimeric crRNA improves CRISPR–Cas12a specificity in the N501Y mutation detection of Alpha, Beta, Gamma, and Mu variants of SARS-CoV-2 Yang, Jun Barua, Nilakshi Rahman, Md. Nannur Lo, Norman Tsang, Tsz Fung Yang, Xiao Chan, Paul K. S. Zhang, Li Ip, Margaret PLoS One Research Article Many CRISPR/Cas platforms have been established for the detection of SARS-CoV-2. But the detection platform of the variants of SARS-CoV-2 is scarce because its specificity is very challenging to achieve for those with only one or a few nucleotide(s) differences. Here, we report for the first time that chimeric crRNA could be critical in enhancing the specificity of CRISPR-Cas12a detecting of N501Y, which is shared by Alpha, Beta, Gamma, and Mu variants of SARS-CoV-2 without compromising its sensitivity. This strategy could also be applied to detect other SARS-CoV-2 variants that differ only one or a few nucleotide(s) differences. Public Library of Science 2021-12-23 /pmc/articles/PMC8700041/ /pubmed/34941928 http://dx.doi.org/10.1371/journal.pone.0261778 Text en © 2021 Yang et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Yang, Jun
Barua, Nilakshi
Rahman, Md. Nannur
Lo, Norman
Tsang, Tsz Fung
Yang, Xiao
Chan, Paul K. S.
Zhang, Li
Ip, Margaret
Chimeric crRNA improves CRISPR–Cas12a specificity in the N501Y mutation detection of Alpha, Beta, Gamma, and Mu variants of SARS-CoV-2
title Chimeric crRNA improves CRISPR–Cas12a specificity in the N501Y mutation detection of Alpha, Beta, Gamma, and Mu variants of SARS-CoV-2
title_full Chimeric crRNA improves CRISPR–Cas12a specificity in the N501Y mutation detection of Alpha, Beta, Gamma, and Mu variants of SARS-CoV-2
title_fullStr Chimeric crRNA improves CRISPR–Cas12a specificity in the N501Y mutation detection of Alpha, Beta, Gamma, and Mu variants of SARS-CoV-2
title_full_unstemmed Chimeric crRNA improves CRISPR–Cas12a specificity in the N501Y mutation detection of Alpha, Beta, Gamma, and Mu variants of SARS-CoV-2
title_short Chimeric crRNA improves CRISPR–Cas12a specificity in the N501Y mutation detection of Alpha, Beta, Gamma, and Mu variants of SARS-CoV-2
title_sort chimeric crrna improves crispr–cas12a specificity in the n501y mutation detection of alpha, beta, gamma, and mu variants of sars-cov-2
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8700041/
https://www.ncbi.nlm.nih.gov/pubmed/34941928
http://dx.doi.org/10.1371/journal.pone.0261778
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